Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection.

The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis-positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.     

Effect of tilmicosin on chemotactic, phagocytic, and bactericidal activities of bovine and porcine alveolar macrophages

OBJECTIVE: To evaluate chemotactic, phagocytic, and bactericidal activities of bovine and porcine alveolar macrophages (AM) exposed to tilmicosin. ANIMALS: 12 healthy calves and 12 healthy pigs. PROCEDURES: Lungs were obtained immediately after euthanasia; AM were collected by means of bronchoalveolar lavage and density gradient centrifugation. Chemotactic activity was evaluated by exposing AM to lipopolysaccharide or macrophage inhibitory peptide during incubation with tilmicosin. Phagocytic activity was evaluated by incubating AM with tilmicosin for 24 hours and then with tilmicosin-resistant Salmonella serotype Typhimurium. Bactericidal activity was evaluated by incubating AM with tilmicosin (0, 10, or 20 microg/ml for bovine AM; 0 or 10 microg/ml or 10 microg/ml but washed free of tilmicosin for porcine AM) and then with Mannheimia haemolytica (bovine AM) or with Actinobacillus pleuropneumoniae or Pasteurella multocida (porcine AM). RESULTS: Tilmicosin had no significant effects on chemotactic or phagocytic activities of bovine or porcine AM. The time-course of bactericidal activity was best described by polynomial equations. Time to cessation of bacterial growth and area under the time versus bacterial number curve were significantly affected by incubation of AM with tilmicosin. CONCLUSIONS AND CLINICAL RELEVANCE: Results show that bactericidal activity of bovine and porcine AM was enhanced by tilmicosin, but not in proportion to the reported ability of AM to concentrate tilmicosin intracellularly. With or without exposure to tilmicosin, the time-course of bactericidal activity of bovine AM against M haemolytica and of porcine AM against A pleuropneumoniae or P multocida was too complex to be reduced to a simple linear equation.     

Taenia solium oncosphere antigens induce immunity in pigs against experimental cysticercosis

Immunity to Taenia solium infection was investigated using an experimental intramuscular oncosphere infection assay (IMOA) model in pigs. Three naturally infected pigs with cysticercosis were treated with oxfendazole (OFZ), a drug demonstrated to kill cysts in porcine muscle. These animals were then challenged with oncospheres but did not develop any cysts while three uninfected pigs that were similarly challenged, did develop intramuscular cysts. In another study, two groups of three pigs each were immunized with crude T. solium oncosphere and metacestode antigens, respectively, and tested with the IMOA. Immunization with crude oncosphere antigens (OAs) induced 100% protection, while metacestode antigens provided only partial protection. Immunoblots showed that pigs with complete immune protection to oncosphere intramuscular challenge had antibodies to two OAs at 31.3 and 22.5 kDa, respectively. Antibody to these two antigens was absent in pigs immunized with metacestodes or in uninfected control pigs. This study demonstrated the presence of two antigens that are unique to the oncosphere. Although, antibody to these two antigens is consistently present in pigs that are protected from an oncosphere intramuscular challenge their role in preventing infection by T. solium larval cysts is still hypothetical.     

Prediction of oriented strand board properties from mat formation and compression operating conditions. Part 2: MOE prediction and process optimization

A model to predict the bending modulus of elasticity (MOE) of oriented strand board (OSB) panels produced by batch processing is presented. The approach developed herein is unique in its comprehensiveness since the MOE is determined from information on the panel structure, temperature and moisture profiles and vertical density profiles obtained from the mat formation and compression models presented in Part 1. Comparison of predicted MOE values with those measured from 24 commercially produced panels shows good agreement considering some of the uncertainties involved. Simulations show that the MOE can be increased by any of the following changes: reduced fines content, increased panel density, better flake alignment in each of the three layers within a panel, increased flake length and a larger difference between the density of the face and core layers. The model was also used in a genetic algorithm to carry out an optimization study of batch OSB manufacturing. This analysis showed that by combining the appropriate reduction in the amount of flakes used, increase in fines content, improvement in flake alignment within each of the face and core layers and shortening of the batch time, a significant theoretical profit increase from the base case scenario can be obtained.     

Protein Requirements of the Freshwater Prawn Macrobrachium rosenbergii Evaluated by the Factorial Method

The dietary protein requirement of the freshwater prawn Macrobrachium rosenbergii to obtain the maximum body protein retention was assessed by the estimated protein needed daily for maintenance (M) and body protein increment (G). The body protein increment was estimated by quantifying body nitrogen increments when the prawn was fed high‐protein diets composed primarily of casein. The protein required for maintenance was estimated by regressing weight gains in feeding experiments using graded dietary levels of casein. The true daily retention (R) or increase in body protein of the prawn corresponds to the sum of M and G. The values of M, G, and R determined in the present study were 3.86, 0.24, and 4.10 g/kg body weight/d, respectively. On the basis of the daily body protein increment and the net protein utilization of casein (58%), dietary protein requirement of the juvenile M. rosenbergii for maximum body protein retention was suggested to be about 7.1 g/kg body weight/d.     

Ranking and classification of irrigation system performance using fuzzy set theory: case studies in Australia and China

A methodology for ranking and classifying performance of irrigation systems through multidimensional performance indicators is developed using fuzzy set theory. The procedure uses the concepts of fuzzy resemblance and fuzzy dominance. Preference levels reflecting management priorities are incorporated into the analysis using appropriate weighting factors. The application of the procedure is demonstrated through two case studies: the Shi-Jin irrigation district in Hebei Province, China and the Goulburn irrigation region in Victoria, Australia. The classification of performance periods for the Australian system shows three clusters indicating the predominant effect of the increase in waterlogged area when higher priority is given to this indicator. No clear trend appeared when equal weight was assigned to all indicators. The effect of economic reforms implemented in 1977–78 in China is clearly reflected in the resulting ranking and clustering of the performance periods in the Shi-Jin irrigation district. Performance levels following reforms are shown to be consistently higher.Units mu unit of area equal to 1/15 ha (666 m²) - jin unit of weight equal to 0.5 kg.     

An integrated hydro-economic modelling framework to evaluate water allocation strategies II: Scenario assessment

In this paper the results of an assessment of the hydrological and economic implications of reallocating water in the Musi sub-basin, a catchment within the Krishna Basin in India, are reported. Policy makers identified a number of different but plausible scenarios that could apply in the sub-basin, involving; supplying additional urban demand from agricultural allocations of water, implementing a number of demand management strategies, changing the timing of releases for hydropower generation, changing the crops grown under irrigation, reducing existing stream flows and allowing for more environmental flows. The framework chosen to undertake this assessment was a simulation model that measures and compares the economic values of water allocation scenarios determined from a water allocation model that accounts for supplies of groundwater and surface water across a number of regions and over a variety of uses. Policy makers are provided with the range of measures on the security of the supply of water and the social costs and benefits of reallocating water between sectors and across regions within the sub-basin. Taking water from agriculture to supply urban users has a greater impact on irrigation supplies during dry years. It was also found that changing the allocation of water between sectors, by taking it away from agriculture had a large positive economic impact on the urban sector. Yet the costs involved in undertaking such a strategy results in a significant loss in the net present value of the scheme. Stream flow reductions, if significantly large (at around 20%), were found to have a large physical and economic impact on the agricultural sector. Implementing water saving strategies in Hyderabad was found to be more cost effective than taking water from agriculture, if rainwater tanks are used to achieve this. Changing the timing of hydropower flows resulted in best meeting of irrigation demand in NSLC and NSRC. Under this scenario, the crops grown under irrigation were found to have a significant economic impact on the sub-basin, but not as large as farmers undertaking crop diversification strategies, ones which result in farmers growing less rice. The security of supplying water to different agricultural zones has significantly improved under this scenario. Finally, releasing water for environmental purposes was found to have only a minor impact on the agricultural sector.     

Fingerprints for main varieties of argentinean wines: terroir differentiation by inorganic, organic, and stable isotopic analyses coupled to chemometrics

Our main goal was to investigate if robust chemical fingerprints could be developed for three Argentinean red wines based on organic, inorganic, and isotopic patterns, in relation to the regional soil composition. Soils and wines from three regions (Mendoza, San Juan, and Co?rdoba) and three varieties (Cabernet Sauvignon, Malbec, and Syrah) were collected. The phenolic profile was determined by HPLC-MS/MS and multielemental composition by ICP-MS; (87)Sr/(86)Sr and δ(13)C were determined by TIMS and IRMS, respectively. Chemometrics allowed robust differentiation between regions, wine varieties, and the same variety from different regions. Among phenolic compounds, resveratrol concentration was the most useful marker for wine differentiation, whereas Mg, K/Rb, Ca/Sr, and (87)Sr/(86)Sr were the main inorganic and isotopic parameters selected. Generalized Procrustes analysis (GPA) using two studied matrices (wine and soil) shows consensus between them and clear differences between studied areas. Finally, we applied a canonical correlation analysis, demonstrating significant correlation (r = 0.99; p < 0.001) between soil and wine composition. To our knowledge this is the first report combining independent variables, constructing a fingerprint including elemental composition, isotopic, and polyphenol patterns to differentiate wines, matching part of this fingerprint with the soil provenance.