氧化塘处理原木蒸煮废水

介绍了原木蒸煮废水的处理方法,提出氧化塘法是小型木材加工厂处理该废水的最佳选择,并用实例简单介绍了氧化塘的设计选型问题.     

浙江天童国家森林公园常绿阔叶林主要组成树种材积与生物量相关关系探讨

以天童国家森林公园常绿阔叶林主要组成树种为研究对象,通过测定其各组分生物量,建立了实测生物量与材积之间的回归模型,结果表明:主要组成树种各组分生物量与其材积之间存在着密切的相关关系,各主要树种材积与生物量的回归模型存在差异;回归方程精度较高,用于根据地区常绿阔叶林主要树种材积推算其生物量是可行的。     

绵羊卵泡发育过程中关键信号通路研究进展

卵泡从原始卵泡发育为成熟卵泡，直至排卵、黄体发育等过程都受到精密的调控，产生大量的优势卵泡是绵羊产多羔及实现快速扩繁的关键因素。研究发现，相关信号通路和转录因子通过影响绵羊卵泡中卵母细胞、颗粒细胞的生长，进而调控卵泡的发育成熟，对这些信号通路进行深入了解，有助于探索卵泡发育的调控机制，早日实现绵羊高效繁育。Notch是卵泡发育过程中发挥重要作用的高度保守信号通路，PI3K/AKT/mTOR信号通路各成员都是广泛存在于细胞内的信号转导分子，在卵泡发育早期发挥了主要作用，还有间隙连接（gap junction，GJ）和跨带突触（transzonal projections，TZPs）等物理连接方式，在细胞间的交流通讯起到重要作用。作者详细介绍了Notch信号通路、PI3K/AKT/mTOR信号通路、间隙连接及跨带突触的结构功能在绵羊卵泡发育中的调控作用，为进一步探明绵羊卵泡发育的调控机制提供参考。     

沉水植物化感作用抑制藻类生长的研究进展

根据国内外相关报道,重点对9科15种沉水植物化感作用物质的构成及抑制15种藻类的生长,沉水植物化感作用抑制藻类生长的机理等方面的研究进展进行综合评述。     

牛GDF-9基因的克隆与组织表达的分析

为了检测牛GDF-9基因mRNA在怀孕期牛生殖系统的表达情况,为GDF-9基因在雌性动物繁殖上的研究提供一定的理论依据。本试验根据GenBank中报道的牛GDF-9基因序列设计引物,从牛卵巢、输卵管、子宫、肾脏中提取RNA,采用RT-PCR技术进行cDNA扩增,扩增产物与载体pGM-T连接,转化大肠杆菌DH5α,筛选阳性克隆,测序。结果表明,已成功克隆的阳性重组子经测序鉴定其片段大小与预期大小完全一致,为264 bp。序列同源性分析比较表明,该cDNA及其推导的氨基酸序列与绵羊GDF-9cDNA和氨基酸序列同源性分别为97%和94%。以-βactin为内参照物,采用RT-PCR技术进行半定量分析,通过检测比较牛GDF-9基因在卵巢、输卵管、子宫、肾脏组织中mRNA的表达情况。结果表明,牛GDF-9基因在卵巢中表达量最高,在输卵管、子宫、肾脏中均有表达,但表达量不高。     

黄浦江上游近自然混交林和人工纯林水源涵养功能评价

以上海黄浦江上游水源涵养林为研究对象,选择近自然混交林、香樟(Cinnamomum camphora)人工纯林和无患子(Sapindus mukorossi)人工纯林,对比了地上部分、枯落物层、土壤层三个垂直层次的持水量,综合评价了其水源涵养功能。结果表明:地上部分持水量近自然混交林与香樟纯林持平,大于无患子纯林,分别为16.36t/hm2,16.85t/hm2,12.54t/hm2;枯落物层最大持水量依次为近自然混交林(0.37t/hm2)无患子纯林(0.004t/hm2)香樟纯林(0.003t/hm2);土壤层最大持水量近自然混交林(2 657.02t/hm2)无患子纯林(2 526.81t/hm2)香樟纯林(2 474.80t/hm2)。土壤层对森林涵养水源功能的贡献最大,但同时离不开地上部分及枯落物层三者间的相互依存和影响。综合评价得到不同类型林分的总持水量为近自然混交林(2 673.73t/hm2)无患子纯林(2 539.35t/hm2)香樟纯林(2 491.65t/hm2)。由此可见,林分复杂、树种多样、林下植被丰富的近自然混交林涵养水源的能力最强,优于单一的无患子纯林,而香樟纯林最差。     

Amplification of Bovine Viral Diarrhoea Virus Introduced into an In Vitro Embryo Production System Via Oocytes from Persistently Infected Cattle

The purpose of this study was to determine whether or not embryos derived from in vitro fertilization of oocytes from persistently infected (PI) cattle would contain infectious virus. Three in vitro embryo production treatment groups were assessed: 1) oocytes and uterine tubal cells (UTC) free of bovine viral diarrhoea virus (BVDV) (negative control), 2) oocytes free of BVDV fertilized and cultured in media containing UTC obtained from PI heifers, and 3) oocytes from PI heifers fertilized and cultured in media containing UTC free of BVDV. The developmental media, UTC and embryos (individual or groups of five) were assayed for virus. Virus was not isolated from any samples in treatment group 1. As shown in previous studies, a proportion of embryo samples were positive for BVDV in treatment group 2. In treatment group 3, the virus associated with the oocytes contaminated the developmental media and infected susceptible co-culture cells used during fertilization and culture. In addition, 65% (11/17) of the degenerated ova from treatment group 3 had infectious virus associated with them. While none of the ova developed into transferable embryos, the study did confirm that use of oocytes from PI cows could lead to amplification of BVDV and cross contamination during in vitro embryo production.     

旱泉沟流域次生林演替过程中种群格局动态

对旱泉沟流域的植被种类组成、物种多样性和物种重要值进行调查分析,采用TWINSPAN对旱泉沟流域次生植物群落进行数量分类,从植物群系组成、植物群落之间的生态关系方面,研究旱泉沟流域封山禁牧区植被群落的分布格局,初步探讨乔木树种的演替趋势。研究结果表明：1）采用TWINSPAN数量分类方法,将植被划分为7个群落类型;2）从群落Ⅰ到群落Ⅶ, 乔木、灌木、草本的Simpson优势度指数基本持平,Shannon Wiener多样性指数为乔木<灌木<草本,Pielou均匀度指数为乔木>草本>灌木,物种丰富度逐渐升高。群落的物种组成和空间结构逐渐复杂化,并趋于稳定;3）次生林的演替序列为山杨树Populus davidiana→桦树Betula platyphylla→青海云杉Pica crassifolia,其自然演替顶级为青海云杉。     

Treatment of a Periarticular Tibial Fracture in a Foal with a Hybrid External Fixator

Objective— To report repair of a right proximal tibial Salter-Harris type II fracture in a foal with a hybrid external fixator (HEF).
Study Design— Case report.
Animals— A 5-month-old male foal.
Methods— After open surgical reduction, an HEF built with wires, 2 half-rings, 3 half-threaded connecting rods, and Schanz pins was used to stabilize the fracture.
Results— Immediately after surgery, the foal had relatively good weight bearing. The HEF was removed at 60 days after radiographic confirmation of healing.
Conclusions— HEF can be used to stabilize a proximal tibial Salter-Harris type II fracture in a 5-month-old foal.
Clinical Relevance— HEF should be considered as another option for repair of proximal tibial Salter-Harris type II fractures in foals.