排序方式: 共有57条查询结果,搜索用时 15 毫秒
1.
Chiho KANEKO Yasuyuki KANEKO Putu Eka SUDARYATMA Hirohisa MEKATA Yumi KIRINO Ryoji YAMAGUCHI Tamaki OKABAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):680
We isolated two pseudorabies virus (PRV) isolates (designated OT-1 and OT-2) from two hunting dogs exhibiting neurological manifestations after eating the flesh of wild boar hunted in Oita prefecture, Kyushu Island, Japan. The isolates corresponded to a previously reported PRV (MY-1 strain) isolated from a hunting dog in neighboring Miyazaki prefecture, and it clustered into genotype II based on the glycoprotein C sequence. Our results suggest that this common PRV strain may have been maintained in wild boars on Kyushu Island even though domestic pigs in this area have attained an Aujeszky’s disease-free status. 相似文献
2.
The principle of time‐resolved fluorometry with lanthanide chelates was established in the 1980s, but in the field of animal sciences it has not been widely applied to immunoassays. However, immunoassays that utilize time‐resolved fluorometry are possible alternatives to radioimmunoassays, since they can attain high sensitivity without safety risks. In this short review, we introduce the development of time‐resolved immunoassays for inhibin A, inhibin B and follicle‐stimulating hormone (FSH), and describe their application to the investigation of FSH regulation in male and female cattle. The results obtained using these newly developed immunoassays indicate that inhibin A acts as a feedback regulator for FSH secretion in female cattle, whereas inhibin A, and probably inhibin B, do so in male cattle. 相似文献
3.
Ehab MOSSAAD Masahito ASADA Daichi NAKATANI Noboru INOUE Naoaki YOKOYAMA Osamu KANEKO Shin-ichiro KAWAZU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):53-58
Bovine babesiosis is a livestock disease known to cause economic losses in
endemic areas. The apicomplexan parasite Babesia bovis is able to invade
and destroy the host’s erythrocytes leading to the serious pathologies of the disease,
such as anemia and hemoglobinuria. Understanding the egress mechanisms of this parasite is
therefore a key step to develop new therapeutic strategies. In this study, the possible
involvement of Ca2+ in the egress of B. bovis merozoites from
infected erythrocytes was investigated. Egress was artificially induced in
vitro using calcium ionophore A23187 and thapsigargin to increase
Ca2+ concentration in the cytosol of the parasite cells. The increased
intracellular Ca2+ concentration following these treatments was confirmed using
live cell Ca2+ imaging with confocal laser scanning microscopy. Based on our
findings, we suggest a Ca2+ signalling pathway in the egress of B.
bovis merozoites. 相似文献
4.
5.
Jun Ohnishi Toshio Kitamura Fumihiro Terami Ken-ichiro Honda 《Journal of General Plant Pathology》2011,77(1):54-59
The ability of the whitefly Bemisia tabaci to transmit two strains of Tomato yellow leaf curl virus, the Israel and Mild strains, was studied after serial transfers of individual whiteflies that were viruliferous for both
strains to tomato plants. After single whiteflies had successive acquisition feedings first on a single plant infected with
one strain and then on a plant infected with the other strain, the single whiteflies later transmitted intermittently one,
the other, or both strains to the test plants during serial transfers at 1-day intervals. Because both strains were found
in the head, abdomen, and legs dissected from whiteflies during the retention period after the two successive acquisition
feedings, both strains apparently circulate from midgut cells to salivary glands through the hemolymph. 相似文献
6.
Change of plasma insulin-like growth factor-1 (IGF-1) concentration with early growth in Japanese beef cattle 总被引:1,自引:0,他引:1
Yoshihito SUDA Kentaro NAGAOKA Kazuki NAKAGAWA Takashi CHIBA Fumihiro YUSA Hisashi SHINOHARA Akira NIHEI Toshihiro YAMAGISHI 《Animal Science Journal》2003,74(3):205-210
The aim of the present study was to examine the change in plasma insulin‐like growth factor‐1 (IGF‐1) concentration with early growth, changes of bodyweight (BW) and relative dairy gain (RDG) in the pre‐ (PRW) and postweaning periods (POW) in Japanese beef cattle, and relationships with metabolites. A total of 33 calves, 22 Japanese black, 6 Japanese shorthorn and 5 of their crossbreed were studied. Insulin‐like growth factor‐1 and metabolite (glucose, triacylglycerol, nonesterified fatty acid) levels in the plasma, from jugular vein blood taken every month, were measured along with BW. Insulin‐like growth factor‐1 in POW increased dramatically with increase of BW (P < 0.05), and the correlation was positive at 0.52 (P < 0.01). Glucose levels correlated significantly with BW, RDG and IGF‐1 (P < 0.01). Metabolic required calorie correlated positively with IGF‐1 (P < 0.01). Also, correlations of BW in POW, with BW and RDG in PRW were positive (P < 0.01). Growth in PRW would be influenced by maternal effects, while active self‐secretion of IGF‐1 in POW might contribute to POW growth. These factors suggested that to increase growth in PRW, maintaining enough maternal effect and IGF‐1 level in POW, was important for establishing better growth after weaning. 相似文献
7.
8.
9.
Kaori KASUGA Maho HIGASHI Takahisa YAMADA Toshie SUGIYAMA Yukio TANIGUCHI Hiroaki IWAISAKI Kosuke HOMMA Yuuichi WAJIKI Yoshinori KANEKO Satoshi YAMAGISHI 《Animal Science Journal》2012,83(1):83-87
The Japanese crested ibis Nipponia nippon is a critically threatened bird. Accurate sexing is necessary to perform effective management of captive breeding toward a national project for a tentative release of the Japanese crested ibis on Sado Island. A PCR‐based sexing method targeting a 0.6 kb EcoRI fragment (EE0.6) sequence on W chromosome with AWS03 and USP3 primers has been developed for the Japanese crested ibis. However, the primers were selected from the EE0.6 sequences from bird species other than the Japanese crested ibis. In this study, we determined the W‐ and Z‐linked EE0.6 sequences in the Japanese crested ibis, and clarified Japanese crested ibis sequence mismatch in the binding sites of the primers. Further, we found no polymorphism in the primer binding sites among five founder birds for the Sado captive Japanese crested ibis population. These findings validated the PCR‐based sexing method with the AWS03 and USP3 as accurate molecular sexing methods of captive Japanese crested ibis on the Sado Island. Additionally, we designed a primer set for a novel PCR‐based sexing, based on the EE0.6 sequences obtained in this study. This novel sexing method may be useful for future ecological research following the release of Japanese crested ibis on Sado Island. This is the first report to show the EE0.6 sequences in Japanese crested ibis. 相似文献
10.
Fuminori TANIHARA Michiko NAKAI Hiroyuki KANEKO Junko NOGUCHI Takeshige OTOI Kazuhiro KIKUCHI 《The Journal of reproduction and development》2013,59(4):385-392
In porcine oocytes, the function of the zona pellucida (ZP) with regard to sperm
penetration or prevention of polyspermy is not well understood. In the present study, we
investigated the effects of the ZP on sperm penetration during in vitro
fertilization (IVF). We collected in vitro-matured oocytes with a first
polar body (ZP+ oocytes). Some of them were freed from the ZP (ZP− oocytes) by two
treatments (pronase and mechanical pipetting), and the effects of these treatments on
sperm penetration parameters (sperm penetration rate and numbers of penetrated sperm per
oocyte) were evaluated. There was no evident difference in the parameters between the two
groups. Secondly, we compared the sperm penetration parameters of ZP+ and ZP− oocytes
using frozen-thawed epididymal spermatozoa from four boars. Sperm penetration into ZP+
oocytes was found to be accelerated relative to ZP− oocytes. Thirdly, we evaluated the
sperm penetration of ZP+ and ZP− oocytes at 1−10 h after IVF (3 h gamete co-incubation).
The proportions of oocytes penetrated by sperm increased significantly with time in both
groups; however, the number of penetrated sperm per oocyte did not increase in ZP−
oocytes. Finally, we performed IVF using ZP− oocytes divided into control (3 h) and
prolonged gamete co-incubation (5 h) groups. Greater numbers of sperm penetrated in the 5
h group than in the control group. These results suggest that the ZP and oolemma are not
competent factors for prevention of polyspermy in our present porcine IVF system. However,
it appears that ZP removal is one of the possibilities for reducing polyspermic
penetration in vitro in pigs. 相似文献