全文获取类型
收费全文 | 639篇 |
免费 | 29篇 |
专业分类
林业 | 56篇 |
农学 | 23篇 |
基础科学 | 1篇 |
73篇 | |
综合类 | 68篇 |
农作物 | 8篇 |
水产渔业 | 16篇 |
畜牧兽医 | 379篇 |
园艺 | 1篇 |
植物保护 | 43篇 |
出版年
2023年 | 8篇 |
2022年 | 6篇 |
2021年 | 8篇 |
2019年 | 9篇 |
2018年 | 6篇 |
2017年 | 14篇 |
2016年 | 8篇 |
2015年 | 11篇 |
2014年 | 9篇 |
2013年 | 33篇 |
2012年 | 29篇 |
2011年 | 20篇 |
2010年 | 22篇 |
2009年 | 15篇 |
2008年 | 12篇 |
2007年 | 24篇 |
2006年 | 22篇 |
2005年 | 22篇 |
2004年 | 10篇 |
2003年 | 19篇 |
2002年 | 17篇 |
2001年 | 9篇 |
2000年 | 8篇 |
1999年 | 12篇 |
1996年 | 8篇 |
1992年 | 13篇 |
1991年 | 18篇 |
1990年 | 18篇 |
1989年 | 16篇 |
1988年 | 13篇 |
1987年 | 17篇 |
1986年 | 22篇 |
1985年 | 12篇 |
1984年 | 10篇 |
1983年 | 14篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1977年 | 9篇 |
1976年 | 5篇 |
1975年 | 8篇 |
1974年 | 11篇 |
1973年 | 9篇 |
1972年 | 4篇 |
1971年 | 10篇 |
1970年 | 8篇 |
1968年 | 4篇 |
1966年 | 11篇 |
1938年 | 6篇 |
排序方式: 共有668条查询结果,搜索用时 15 毫秒
1.
2.
Background
Invasive Australian populations of redlegged earth mite, Halotydeus destructor (Tucker), are evolving increasing organophosphate resistance. In addition to the canonical ace gene, the target gene of organophosphates, the H. destructor genome contains many radiated ace-like genes that vary in copy number and amino acid sequence. In this work, we characterise copy number and target-site mutation variation at the canonical ace and ace-like genes and test for potential associations with organophosphate insensitivity. This was achieved through comparisons of whole-genome pool-seq data from alive and dead mites following organophosphate exposure.Results
A combination of increased copy number and target-site mutations at the canonical ace was associated with organophosphate insensitivity in H. destructor. Resistant populations were segregating for G119S, A201S, F331Y at the canonical ace. A subset of populations also had copy numbers of canonical ace > 2, which potentially helps overexpress proteins carrying these target-site mutations. Haplotypes possessing different copy numbers and target-site mutations of the canonical ace gene may be under selection across H. destructor populations. We also detected some evidence that increases in copy number of radiated ace-like genes are associated with organophosphate insensitivity, which might suggest potential roles in sequestration or breakdown of organophosphates.Conclusion
Different combinations of target-site mutations and (or) copy number variation in the canonical ace and ace-like genes may provide non-convergent ways for H. destructor to respond to organophosphate selection. However, these changes may only play a partial role in organophosphate insensitivity, which appears to have a polygenic architecture. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献3.
Benjamin D. Hoffmann Adam Tessmann Greg Quinn Faye Lawton 《Pest management science》2023,79(6):2050-2055
BACKGROUND
Aerial treatments for invasive species management are now common, but we are unaware of any work published in the scientific literature quantifying how the interplay of numerous factors affects flight time and therefore operational costs. Here, we analyse aerial treatment data collected from two ant-eradication programmes, quantifying how the relationships between flight time and area are influenced by numerous aircraft/delivery system/bait/flight speed combinations.RESULTS
For bait dispersal by helicopters, and when swath widths are equivalent, side-mounted Isolair was significantly more efficient than the simultaneous use of two underslung buckets, and use of two buckets was slightly but not significantly more efficient than one bucket. In this scenario, delivery by Isolair was, on average, 39.8% and 31.5% more efficient than the use of one or two buckets, respectively. However, when the swath width used with Isolair was halved to 10 m and flight speed was increased slightly, flight time was significantly greater compared to the other configurations. For bait dispersed by drone, flights conducted using an upgraded Flight Management System (FMS) and greater flight speed but smaller swath width were significantly more efficient than flights using the older FMS and lower flight speed. Over 10 and 50 ha the helicopter was 2.87 and 4.82 times more time efficient than the drone.CONCLUSION
We encourage practitioners to publish data from their aerial treatments, and to try new methods, to accelerate improvements in efficiency and reduce the costs of aerial treatments. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献4.
Effects of different forms and origins of oilseeds on dynamics of ruminal biohydrogenation of long‐chain fatty acids in vitro 下载免费PDF全文
A. Hoffmann H. Steingass M. Schollenberger H. Terry K. Hartung E. Weiss R. Mosenthin 《Journal of animal physiology and animal nutrition》2015,99(6):1031-1038
Dietary unsaturated fatty acids (FA) are intensively hydrogenated in the rumen, resulting in reduced amount of poly‐unsaturated fatty acids (PUFA) and accumulation of several biohydrogenation (BH) products. In this study, BH of PUFA originating from different oilseeds (linseed, soya beans, sunflower seed and rapeseed) present in crushed oilseeds or their free oils were assessed in vitro. The assay substrates were incubated in buffered rumen fluid for 0, 6, 12 and 24 h. After incubation, the FA pattern of the incubated samples was analysed using gas chromatography. Biohydrogenation is defined as disappearance of double bonds (DB) calculated from the contents of unsaturated FA. After 24‐h incubation, the DB contents of all oilseeds were reduced (p < 0.001) by 40–60%. The reduction was higher (p < 0.001) for the crushed form compared with the oil form. In addition, linseed and sunflower seed known as oilseeds with high contents of linolenic acid C18:3 c9,12,15 (LNA) and linoleic acid C18:2 c9,12 (LA), respectively, showed a higher (p < 0.001) accumulation of the BH intermediates conjugated linoleic acid (CLA, isomer C18:2 c9t11) and vaccenic acid (C18:1 t11) for the crushed form, when compared with the oil. These results suggest an inherent effect of the physical form of the assay oilseeds on in vitro BH. Changes in FA pattern during BH in vitro can be attributed to both source and physical form of the assay oilseeds. However, further investigations are warranted to ensure whether the observed in vitro effects on ruminal BH can be confirmed in vivo. 相似文献
5.
Relevance of Osmotic and Frost Protecting Compounds for the Winter Hardiness of Autumn Sown Sugar Beet 下载免费PDF全文
The cultivation of autumn sown sugar beet (winter beet) is supposed to result in a marked yield increase compared with spring sown sugar beet. Although the importance of the growth stage reached before winter for the survival of autumn sown sugar beet has already been shown, it is not clear to which extent osmotic and potentially frost protecting compounds may contribute to winter hardiness. The study thus aimed to analyse the acclimatization process of sugar beet to low temperatures and to identify compounds which are important for survival of frost. Field trials with autumn sown sugar beet were conducted at eleven environments in Germany from 2009/10 to 2012/13, which were accompanied by greenhouse experiments with controlled temperature regimes. In the field trials, the survival rates after winter varied from 0 % to 99 %, but only in four environments differences between the five genotypes occurred. During acclimatization, betaine, glutamine, proline and raffinose were markedly accumulated and osmolality was enhanced. In particular betaine, amino acids and osmolality showed a positive correlation to the survival rate and were thus identified as potentially frost protecting substances for sugar beet. In contrast, raffinose and proline seem to act rather as stress indicators as they were negatively related to survival. Possible frost protecting substances were identified which can be used in breeding to improve the winter hardiness of sugar beet. 相似文献
6.
The first International Technical Conference on Animal Genetic Resources for Food and Agriculture, organized by the Food and Agriculture Organization of the United Nations, took place from 3 to 7 September, 2007, in Interlaken, Switzerland. The main achievement of the Conference was the adoption of the Global Plan of Action for Animal Genetic Resources, the first ever international framework for the promotion of the wise management of animal genetic resources for food and agriculture, endorsed by governments. The article describes the technical and intergovernmental processes developing the Global Plan of Action. The adoption of the Global Plan of Action has created unprecedented momentum for promoting the sustainable use, development and conservation of the world's livestock diversity. The international community is now facing the challenge to ensure that the Global Plan of Action is effectively implemented. 相似文献
7.
8.
Agr.-Ing. M. Hoffmann 《Journal of pest science》1986,59(5):100-100
A seldom position of the body of swimmingO. zibethica (s. figure) was observed twice in ponds near Ilmenau/Thuringia and Bautzen/Saxony. 相似文献
9.
Max Hoffmann 《Journal of pest science》1970,43(5):74-75
Ohne Zusammenfassung 相似文献
10.
Tiphaine Dubos Matias Pasquali Friederike Pogoda Lucien Hoffmann Marco Beyer 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(2):239-248
A collection of 55 Fusarium graminearum (Gibberella zeae) strains isolated between 1969 and 2009 in Belgium, Canada, Germany, Italy, Luxembourg, or the USA belonging to the three
known chemotypes (3-acetylated deoxynivalenol, 15-acetylated deoxynivalenol and nivalenol) were screened for their sensitivity
towards the fungicide trifloxystrobin using a liquid culture assay. None of the isolates was completely inhibited by trifloxystrobin
concentrations up to 3 mM. For comparison, prothioconazole completely inhibited fungal growth of a standard isolate at concentrations
as low as 0.007 mM. The maximum level of inhibition, which could be obtained by trifloxystrobin, ranged from 14 to 65% among
the strains tested and was not significantly affected by the country of origin or by the chemotype. The absence of significant
differences in resistance levels between the countries of origin and chemotypes as well as the fact that strains isolated
before the market introduction of strobilurins in 1996 also showed a high level of resistance is evidence that this is largely
a case of natural resistance and not primarily related to strobilurin use in agriculture. 相似文献