Characterization of Competitive Mycelial Growth in Pleurotus eryngii

Competitive mycelial growth(CMG)between Pleurotus eryngii isolates is genetically controlled,and stable CMG responses(positive,neutral or negative)were recorded among the strains tested.Correlations between CMG responses and mycelial growth rate were observed.CMG simultaneously reflects information relating to somatic incompatibility and mycelia growth rate in edible mushrooms,and can be readily and reliably applied to studies on genetic polymorphism among this group of fungi.     

Cloning and analysis of cross-intron genomic gene encoding ACO in Paeonia suffruticosa Andr.

The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by ACC oxidase (ACO). In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from Paeonia suffruticosa. It revealed that PsgACO (FJ855434) has 1281 bases, containing four exons and three introns, encoding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm.     

Comparative Studies on the Stability of Various Forms of vitamin C

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Biodegradation of Linear Alkyl Benzene Sulphonate

The paper researches on the degradation of LAS (Linear Alkyl benzene Sulphonate) by combining AS1.860 immobilized with low intensity ultrasonic irradiation. The paper also discusses the influence of pH, rotary velocity, LAS concentration and the condition of low intensity ultrasonic irradiation on the degradation of LAS, the degradation rate of LAS is the main index of our experiment, the results of orthogonal test shows that low ultrasonic irradiation can increase the metabolizing of microorganism cells and facilitate the biodegradation of immobilized cells to LAS, here we research the degradation condition of 50mg/L LAS simulation wastewater with low ultrasonic irradiation, and the results show that the influence is obvious, the optimization degradation rate is about 83%, nine point five percent higher than that of the immobilized cells without ultrasonic irradiation.     

Biocompatibility of Acellular Porcine Dermis

In vitro cultured vascular endothelial cells (VEC) and mouse 3T3 fibroblasts (3T3) on the acellular dermal matrix , which were made of porcine skins. We made the cell proliferation test with MTT assay and the histological observation after cells were seeded on the acelluar dermis for 1 week with histological section. The cell growth curves showed VEC and 3T3 grew much rapidly on the acelluar dermis.The histological observation revealed VEC had formed a monolayer, some places even had formed 2 to 3 layer. The results suggest that the acelluar porcine dermal matrix have good biocompatibility , it will be widely applied.     

Experimental Study on the Deformation Characteristics of Fine-Sandstone Under the Loading and Unloading Conditions

The deformation characteristics of the fine-sandstone is studied under the loading and unloading conditions at the three different deformation-rates and the two different stress-levels. The experimental results show that there is really the plastic-loop in the deformation curve for the loading deformation curve is different from the unloading deformation curve. But the plastic-loop is nearly unchangeable since the second circulate loading. The residual-deformation is be almost zero since the second circulate loading, and that the slope of the deformation curve increases as the deformation-rate increases, and that the residual-deformation increases as the stress-levels increases.     

PROBABILISTIC EVALUATION FOR STEEL FRAME COLUMNS OF TALL BUILDINGS

Based on properties of parameters and test data of dom-estic steel,this paper analyses the reliability and partial coefficient forresistance of steel frame columns of tall builings.The result may serveas a scientific basis.It is not only for domestic steel instead of importedsteel in building tall steel structures,but also for compiling concerendstandards.