Using knowledge of behaviour and optic physiology to improve fish passage through culverts

Culverts reduce connectivity for aquatic animals by being both a hydraulic and physical barrier. However, altered light intensity may also be a behavioural barrier to fish movement, especially for diurnal species that have adapted to moving when it is light. We propose that knowledge of optical physiology and fish behaviour, two important mechanisms underpinning movement, can inform efforts to improve fish movement through culverts. We firstly review the sensory systems of fish with reference to visual sense and explore how this affects fish movement. We then highlight theoretical knowledge that can help us understand fish behaviour and the potential mismatch between the conditions under which fish have evolved and altered conditions within culverts. We describe potential knowledge gaps and directions for future research to improve our understanding of how culverts may affect fish movement. Finally, we explore the potential costs and ecological benefits of different mitigation options to identify those with the most promise for managing the light environment in culverts to facilitate movement. For researchers and managers exploring this subject, we suggest an approach that: (a) identifies light requirements for movement by different species, (b) tests movement under different light conditions and (c) considers an integrative assessment method for testing fish behaviour around culverts. Understanding how optical physiology, fish behaviour and culvert design influence fish movement can improve connectivity for a range of species.     

Macroalgal meadow habitats support fish and fisheries in diverse tropical seascapes

Canopy‐forming macroalgae can construct extensive meadow habitats in tropical seascapes occupied by fishes that span a diversity of taxa, life‐history stages and ecological roles. Our synthesis assessed whether these tropical macroalgal habitats have unique fish assemblages, provide fish nurseries and support local fisheries. We also applied a meta‐analysis of independent surveys across 23 tropical reef locations in 11 countries to examine how macroalgal canopy condition is related to the abundance of macroalgal‐associated fishes. Over 627 fish species were documented in tropical macroalgal meadows, with 218 of these taxa exhibiting higher local abundance within this habitat (cf. nearby coral reef) during at least one life‐history stage. Major overlap (40%–43%) in local fish species richness among macroalgal and seagrass or coral reef habitats suggest macroalgal meadows may provide an important habitat refuge. Moreover, the prominence of juvenile fishes suggests macroalgal meadows facilitate the triphasic life cycle of many fishes occupying diverse tropical seascapes. Correlations between macroalgal canopy structure and juvenile abundance suggests macroalgal habitat condition can influence levels of replenishment in tropical fish populations, including the majority of macroalgal‐associated fishes that are targeted by commercial, subsistence or recreational fisheries. While many macroalgal‐associated fishery species are of minor commercial value, their local importance for food and livelihood security can be substantial (e.g. up to 60% of landings in Kenyan reef fisheries). Given that macroalgal canopy condition can vary substantially with sea temperature, there is a high likelihood that climate change will impact macroalgal‐associated fish and fisheries.     

Assessing the sublethal impacts of anthropogenic stressors on fish: An energy‐budget approach

Fish are increasingly exposed to anthropogenic stressors from human developments and activities such as agriculture, urbanization, pollution and fishing. Lethal impacts of these stressors have been studied but the potential sublethal impacts, such as behavioural changes or reduced growth and reproduction, have often been overlooked. Unlike mortality, sublethal impacts are broad and difficult to quantify experimentally. As a result, sublethal impacts are often ignored in regulatory frameworks and management decisions. Building on established fish bioenergetic models, we present a general method for using the population consequences of disturbance framework to investigate how stressors influence ecologically relevant life processes of fish. We partition impact into the initial energetic cost of attempts to escape from the stressor, followed by the energetic impacts of any injury or behavioural change, and their consequent effects on life processes. As a case study, we assess the sublethal effects of catch and release angling for the European sea bass (Dicentrachus labrax, Moronidae), a popular target species for recreational fishers. The energy budget model described is not intended to replace existing experimental approaches but does provide a simple way to account for sublethal impacts in assessment of the impact of recreational fisheries and aid development of robust management approaches. There is potential to apply our energy budget approach to investigate a broad range of stressors and cumulative impacts for many fish species while also using individual‐based models to estimate population‐level impacts.     

Assessment of molecular detection of Fusarium circinatum in insects and passive spore traps in Pinus radiata plantations

Fusarium circinatum is the causal agent of pitch canker, a destructive disease that threatens natural and planted pine forests around the world. Although pitch canker has caused problems in Spain and Portugal, concerning Europe as a whole, the fungus is not established across the pine distribution area. Its dispersion by wind and/or insect vectors could nevertheless play a role in the colonization of currently uninfected stands. It is therefore crucial to develop monitoring tools for its detection. To this end, we assessed the molecular detection of the pathogen in environmental samples of bark beetles and passive spore traps, collected in two infected Pinus radiata plantations in Basque country, Spain. The spread pattern of F. circinatum was assessed by an experimental design that included insect and spore traps installed at the centre, at the edge and outside the plots. Our results showed that F. circinatum was detected in both types of samples, at almost all collection dates. In both type of samples, positive detections were mainly found at the centre of the plots, a lower proportion at the edge, and very few outside. This suggests that long‐distance dispersion of Fusarium circinatum does not rely on wind spore dispersal neither on insect flight. Our study also shows that molecular methods are a powerful tool to monitor the pathogen in environmental samples.     

Selecting the hologenome to breed for an improved feed efficiency in pigs—A novel selection index

Most traits in animal breeding, including feed efficiency traits in pigs, are affected by many genes with small effect and have a moderately high heritability between 0.1 and 0.5, which enables efficient selection. Since the microbiota composition in the gastrointestinal tract is also partly heritable and was shown to have a substantial effect on feed efficiency, the host genes affect the phenotype not only directly by altering metabolic pathways, but also indirectly by changing the microbiota composition. The effect of the microbiota composition on the breeding value of an animal is the conditional expectation of its breeding value, given the vector with microbiota frequencies, that is The breeding value of an animal can therefore be decomposed into a heritable contribution that arises from an altered microbiota composition and a heritable contribution that arises from altered metabolic pathways within the animal, so Instead of selecting for breeding value , an index comprising the two components and with appropriate weights, that is , can be used. The present study shows how this breeding strategy can be applied in pig genomic selection breeding scheme for two feed efficiency traits and daily gain.     

The Warrumbungle Post‐Fire Recovery Project—raising the profile of soils

The impacts of a wildfire and subsequent rainfall event in 2013 in the Warrumbungle National Park in New South Wales, Australia were examined in a project designed to provide information on post‐fire recovery expectations and options to land managers. A coherent suite of sub‐projects was implemented, including soil mapping, and studies on soil organic carbon (SOC) and nitrogen (N), erosion rates, groundcover recovery and stream responses. It was found that the loss of SOC and N increased with fire severity, with the greatest losses from severely burnt sandstone ridges. Approximately 2.4 million t of SOC and ~74,000 t of N were lost from soil to a depth of 10 cm across the 56,290 ha affected. Soil loss from slopes during the subsequent rainfall event was modelled up to 25 t ha^?1, compared to a long‐term mean annual soil loss of 1.06 t ha^?1 year^?1. Groundcover averages generally increased after the fire until spring 2015, by which time rates of soil loss returned to near pre‐fire levels. Streams were filled with sand to bank full levels after the fire and rainfall. Rainfall events in 2015–2016 shifted creek systems into a major erosive phase, with incision through the post‐fire sandy bedload deposits, an erosive phase likely related to loss of topsoils over much of the catchment. The effectiveness of the research was secured by a close engagement with park managers in issue identification and a communications programme. Management outcomes flowing from the research included installation of erosion control works, redesign of access and monitoring of key mass movement hazard areas.     

High‐throughput Cryopreservation of Sperm from Sex‐reversed Southern Flounder,Paralichthys lethostigma

The Southern flounder, Paralichthys lethostigma, is a valuable aquaculture fish with established markets in the USA. All‐female production in this species is an important technology for aquaculture because the females usually have body sizes twice those of males at the same age, and sex‐reversed males (genotypic XX neomales) are used for all‐female production by crossing with genetically normal females. However, sperm volume from the neomales is usually small (<0.5 mL) and limits their application for all‐female fish production. Cryopreservation of sperm from these sex‐reversed neomales will provide access on demand with increased efficiency to extend the application of neomales. The goal of this study was to develop a protocol for cryopreservation of sperm from the Southern flounder by using an automated high‐throughput processing system. The objectives were to: (1) determine the effect of osmolality on activation of sperm motility; (2) evaluate the effect of extender solutions on sperm motility capacity; (3) evaluate the acute toxicity of cryoprotectants (dimethyl sulfoxide [DMSO], propylene glycol, and polyethylene glycol) on sperm motility, and (4) estimate the effect of cooling rate on sperm cryopreservation and post‐thaw fertilization. Sperm motility was activated when osmolality was 400 mOsmol/kg or higher. Of the three extender buffers tested, HEPES4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES) at 300 mOsmol/kg resulted in better protection for sperm motility than did Hanks' balanced salt solution and Mounib solution at 300 mOsmol/kg during 7 d of refrigerated storage. After 30 min equilibration with the cryoprotectant of 15% DMSO, sperm motility was 24 ± 21% (fresh sperm motility without any cryoprotectants was 42%). After cooling at a rate of 20 C/min, post‐thaw sperm motility was 8 ± 5% and fertilization was 63 ± 40% evaluated at the 32–64 cell stage (5 × 10⁵ sperm per egg). Overall, a protocol was developed for sperm cryopreservation in the Southern flounder with high‐throughput processing, which provides a tool to preserve the valuable genetic resources from neomale flounders, and enables germplasm repository development for the Southern flounder.     

Oxidative Stress and Interaction of Endothelin Receptors in Airways of Clinically Healthy Horses

Endothelin-1 (ET-1), a physiological as well as an inflammatory mediator, causes contraction of airway smooth muscles by binding to endothelin-A (ETA) and endothelin-B (ETB) receptors. We investigated the interaction between endothelin receptors on contractions and oxidative stress of bronchial rings of clinically healthy horses. Rings for response studies were set in tissue chambers containing oxygenated Tyrode’s solution. Concentration-response relationships were determined for ET-1 on control rings, rings incubated with ETA antagonist (BQ123), rings incubated with ETB antagonists (IRL 1038 and BQ788), and rings incubated with all antagonists combined. For oxidative stress studies, rings were incubated for 30 minutes separately with ET-1, ETA antagonist + ET-1, ETB antagonists + ET-1, and all three antagonists + ET-1. The control rings were not treated with any agents. Electron paramagnetic resonance (EPR) method was used to measure total reactive oxygen species (ROS), superoxide, and peroxynitrite. Results showed that ET-1 caused dose-dependent contractions which were increased by ETA blockade and decreased by ETB blockade. Combined blockade significantly inhibited the response to ET-1. Regarding the oxidative stress, ET-1 and its antagonists significantly reduced the total ROS. Superoxide production was significantly decreased by ETA antagonist and completely abolished when ETA and ETB antagonists were combined. Peroxynitrite production was not changed significantly. The study suggested that an interaction between ET receptors exists in the expression of contractile responses and oxidative stress. ET-1 attenuated oxidative stress by decreasing total ROS. ETA receptors may be primarily responsible for superoxide production. It appears that ET-1 does not affect peroxynitrite production in normal equine tissues.     

Trunk inoculations of Phytophthora cinnamomi in red oaks

Trunks of northern red oaks were inoculated with Phytophthora cinnamomi. To study lesion growth in bark tissues over several years, lesion assessment methods were perfected and tested. Koch's postulates were demonstrated. Development and longevity of lesions were studied. Intra-specific variability of red oak susceptibility was investigated.