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排序方式: 共有192条查询结果,搜索用时 15 毫秒
1.
Trent PerryJanice Q. Chan Phil BatterhamGerald B. Watson Chaoxian GengThomas C. Sparks 《Pesticide biochemistry and physiology》2012,102(1):56-60
Several strains of Drosophila melanogaster possess mutant alleles in nicotinic acetylcholine receptor (nAChR) subunits, Dα1 and Dβ2 that confer resistance to neonicotinoids such as imidacloprid and nitenpyram, and Dα6, that confers resistance to spinosyns. These mutant strains were bioassayed with a selected set of nAChR active insecticides including neonicotinoids, spinosad, and sulfoxaflor, a new sulfoximine insecticide. All of the neonicotinoids examined, except dinotefuran showed reduced insecticidal efficacy on larvae of the Dα1 mutant, suggesting that this subunit may be important in the action of these insecticides. All of the neonicotinoids, including dinotefuran, showed reduced insecticidal efficacy on larvae possessing the Dβ2 mutation. A similar pattern of broad neonicotinoid resistance to that of Dβ2 alone was also observed for larvae with both the mutations (Dα1 + Dβ2). The Dβ2 mutation exhibited a lower level of cross-resistance to sulfoxaflor (<3-fold) than to any of the neonicotinoids (>13-fold). In contrast, there was no cross-resistance for any of the neonicotinoids or sulfoxaflor in adult flies with the Dα6 mutation, which confers high levels of resistance to spinosad. Thus in the D. melanogaster strains studied, target site resistance observed for the neonicotinoids and the spinosyns does not translate directly to resistance towards sulfoxaflor. 相似文献
2.
Taek-Ryoun Kwon Zamin Shaheed Siddiqui Phil J.C. Harris 《Journal of plant nutrition》2013,36(4):644-667
ABSTRACT Effects of three supplemental calcium (Ca++; 2.5, 5.0, and 10 mole m?3) concentrations on ion accumulation, transport, selectivity, and plant growth of salt-sensitive species, Brassica rapa ‘Sani’ in saline medium were investigated. Supplemental Ca++ in the presence of 125 mol m?3 sodium chloride (NaCl) did not improve the dry weight and leaf area indicating no role played by Ca++ in the alleviation of salinity induced growth inhibition. However, calcium chloride (CaCl2) did significantly affect sodium (Na+), potassium (K+), and Ca++ contents of roots and shoots. The ion contents of shoots were significantly greater than those of roots per g dry weight, indicating ion transportation to shoots is greater than ion accumulation in roots. Use of CaCl2 in 125 mol m?3 NaCl reduced the Na+ content but increased K+ and Ca++ contents in shoots. Sodium contents in shoots differed among the supplemental Ca++ treatments indicating the role of CaCl2 in Na+ ions transportation. Calcium content in shoots declined significantly in the control treatment (0 CaCl2) but increased significantly in 10 mol m?3 CaCl2. The root also showed the effects of Ca++ on the reduction of Na+ content and the increase of K+ and Ca++ content. Unexpectedly, 5 mol m?3 CaCl2 induced the highest Na+ content in roots at 16 days after treatment. Supplemental CaCl2 application influenced the K+ or Ca++ selectivity over Na+ in two ways, ion accumulation at roots and transport to shoots. However, high CaCl2 treatments allowed greater Ca++ selectivity over Na+ than low CaCl2. Likewise, high supplemental CaCl2 showed higher K+ selectivity over Na+ than low CaCl2. A marked increase in K+ versus Na+ selectivity for the transport process occurred at 10 mol m?3 CaCl2 treatments. The roots and shoots exhibited higher K+/Na+ and Ca++/Na+ ratios in high CaCl2 treatment than in low. The results are discussed in context to supplemental Ca++ concentrations, ions accumulation, transportation and selectivity of salt sensitive Brassica rapa cultivar. 相似文献
3.
Jundae Lee Jae Bok Yoon Jung-Heon Han Won Phil Lee Sang Hoon Kim Hyo Guen Park 《Euphytica》2010,173(1):55-61
Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms
3
gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP)
techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc276, Eagc/Mctt178, and Ecag/Mtgc204) were identified as tightly linked to the ms
3
locus. Among them, we converted the AFLP marker Ecag/Mtgc204 to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and
flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using
the GMS system. 相似文献
4.
Hot water extract (HWE) from Pinus radiata bark was fractionated into monomeric polyphenol (MPP), oligomeric proanthocyanidin (OPA), and polymeric proanthocyanidin
(PPA) fractions by monitoring the UV-visible spectrum of the eluted fractions. Nine polyphenols were identified in MPP: three
phenolic acids and six flavonoids. The major compounds of MPP were taxifolin (456 μg/mg), catechins (240 μg/mg), and protocatechuic
acid (46.2 μg/mg). The OPA components ranged from dimers to hexamers. PPA comprised procyanidin (PC, 94%), a small amount
of prodelphinidin (PD, 6%), and also the higher trans configuration, rather than the cis configuration, of C2-C3. The average molecular weight (Mw, Mn), polydispersity (Mw/Mn), and DP of PPA were 3,800 (Mw), 1,200
(Mn), 3.2 and 13, respectively. The potential antioxidant activities of HWE and the three fractions were estimated using proton-
or electron-donating assays containing 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate)
(ABTS) radical cation, superoxide anion (O2•−), hydrogen peroxide (H2O2), reducing power and lipid peroxidation. All the bark fractions exhibited potent quenching abilities against both commercially
available radicals (DPPH•, ABTS•+) and chemically induced radicals (H2O2, O2•−), as well as strong reducing power and inhibitory activity of lipid peroxidation. Particularly, OPA showed a hydrogen peroxide
scavenging activity significantly higher (p < 0.05) than that of the other fractions. From 2-thiobarbituric acid reactive substances and ferric thiocyanate assays, PPA
was suspected to act by essentially delaying the formation of hydroperoxide by quenching free oxygen radicals that operates
in oil-in-water emulsion. 相似文献
5.
Collective action by local communities has been recognised as crucial for effective management of natural resources, particularly
the management of forests in rural settings in developing countries. However, the processes and outcomes of collective action
in forest management are often analysed through a narrow rational choice model, ignoring the impacts of wider social, political
and economic processes in conditioning peoples’ decisions to act (or not to act) collectively. Optimistic assumptions are
made for collective action being instrumental to enhance both social and ecological outcomes, but there is a paucity of empirical
evidence on how and why the condition of forests has improved (or deteriorated) under collective action, and what impacts
the change in forest condition has on various groups within local communities. This study critically examines the emergence,
evolution and outcomes of collective action in a case of community forestry in Nepal. A mix of qualitative and quantitative
methods has been used to collect primary data from the forest, households, key informants and focus groups. The emergence
and outcomes of collective action is found to be embedded in social, economic and political relationships, where powerful
actors control the use of forests in order to ensure conservation, thereby resulting in the underutilisation of forest products.
Poor users, who depend heavily on forests, are found to be worse off economically under community forestry, but still engage
in collective action for a variety of socio-political reasons. This contradicts the conventional wisdom which assumes that
people only cooperate when they benefit from cooperation. It is concluded that a deeper understanding of the embeddedness
of community forestry is needed in order to achieve the potential of collective action.
相似文献
Krishna K. ShresthaEmail: |
6.
Pine (Pinus densiflora) bark was chemically modified by grafting with acrylonitrile (AN) through Fenton reaction and conversion of the cyano group
to an amidoxime group by reaction with hydroxylamine. Treatments of pine bark with 160 mM H2O2 at 40°C yielded ~70% grafting in 6-h reaction. After alkali hydrolysis of amidoximated pine bark (AOPB), its adsorption capacity
(q
e) for copper ions was largely increased from 0.5 to 1.8 mmol/g. Adsorption of the hydrolyzed AOPB (HAOPB) followed the pseudo-second-order
kinetic model. Among the metal ions tested, the order of adsorption capacity (q
e) predicted by the kinetic model at equilibrium time was Hg2+ > UO2
2+ > Cd2+ > Cu2+ > Zn2+ > Ni2+. The adsorption process was pH-dependent, and the adsorption equilibrium was observed in the pH range of 6–7 for all the
metal ions. Even though pine bark is a good adsorbent for Hg2+ and UO2
2+ ions, it had a poor affinity for the other ions tested. It was obviously found that HAOPB had very high adsorption abilities
for heavy metal and uranyl ions. 相似文献
7.
Thomas J. Drinan Phil McGinnity Jamie P. Coughlan Tom F. Cross Simon S.C. Harrison 《Ecology of Freshwater Fish》2012,21(3):420-432
Abstract – The morphologies of sympatric juvenile Atlantic salmon Salmo salar parr and brown trout Salmo trutta parr were compared between Irish rivers with contrasting hydraulic environments – a high‐gradient and a low‐gradient tributary from the River Barrow catchment, south‐east Ireland and a high‐gradient river from the Burrishoole catchment, west of Ireland. The two catchments differ markedly in mean annual precipitation (849.0 mm year?1 and 1370.3 mm year?1 for the Barrow and Burrishoole catchments, respectively). Parr of both species demonstrated morphological variation between and within catchments. Changes in metrics such as pectoral fin length, body depth and body length between and within catchments suggest that hydraulic forces were a major determinant of morphological variation. Both species from the Burrishoole catchment had relatively larger pectoral fins, longer heads, larger eyes and shallower bodies than conspecifics from the Barrow catchment. In rivers subject to frequent rainfall‐driven high discharges, such as those in the Burrishoole catchment, a more fusiform body and head shape coupled with larger pectoral fins may reduce the energetic cost of maintaining position in the water column, as well as increase stability and manoeuvrability. The larger eyes in both parr species in the Burrishoole catchment could further be a response to the lower visibility of the more turbid and peat‐stained waters or to the reduced prey availability. The results of this study demonstrate that local adaptation to flow conditions can yield morphologically distinct populations and that multiple species can exhibit parallel phenotypic responses to changing environmental conditions. 相似文献
8.
Mairead Shore Phil Jordan Per-Erik Mellander Mary Kelly-Quinn Karen Daly James Tom Sims David P. Wall Alice Rowena Melland 《Journal of Soils and Sediments》2016,16(5):1643-1654
Purpose
This study investigated the phosphorus (P) source, mobilisation and transport potential of ditch bed sediments as well as surrounding field and bank soils in two agricultural headwater catchments with contrasting soil drainage capacities. This information is important for discerning the potential for ditches to attenuate or augment transfers of P from upstream sources and thus for developing appropriate management strategies for these features.Materials and methods
Phosphorus sources were characterised using the Mehlich3-P, water-soluble P and total P tests. Phosphorus mobilisation potential was characterised using the Mehlich3-AL/P, Mehlich3-Ca/P and DESPRAL P tests. Phosphorus transport potential was characterised using data collected on the presence/absence of surface water in ditches during field surveys and downstream turbidity data.Results and discussion
Ditch sediments had similar P source contents (Mehlich3-P, water-soluble P and total P) to the surrounding field soils and higher P contents than bank soils. However, calcium contents of sediments in the poorly drained catchment reflected the deep sub-soils rather than the surrounding field and bank soils. Mehlich3-Al/P and Mehlich3-Ca/P contents of ditch sediments in the well (non-calcareous) and poorly (calcareous) drained catchments respectively indicated potential for P retention (above thresholds of 11.7 and 74, respectively). However, sediments were less aggregated than field soils and may mobilise more particulate P (PP) during rain events. Nevertheless, the majority of surveyed ditches dried out from March to September 2011; thus, their potential to mobilise PP may be less important than their capacity to attenuate soluble and PP during this time.Conclusions
In these and similar catchments, soluble P attenuation and particulate P mobilisation should be maximised and minimised, respectively, for example, by cleaning out the sediments before they become saturated with P and encouraging vegetation growth on ditch beds. This study also highlighted the influence of deep sub-soils on soluble P retention in ditches and thus the utility of characterising soils below depths normally included in soil classifications.9.
Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite,Neoparamoeba perurans,on Atlantic salmon 下载免费PDF全文
Daniel William Wright Barbara Nowak Frode Oppedal Phil Crosbie Lars Helge Stien Tim Dempster 《Journal of fish diseases》2018,41(9):1403-1410
Freshwater bathing is one of the main treatment options available against amoebic gill disease (AGD) affecting multiple fish hosts in mariculture systems. Prevailing freshwater treatments are designed to be long enough to kill Neoparamoeba perurans, the ectoparasite causing AGD, which may select for freshwater tolerance. Here, we tested whether using shorter, sublethal freshwater treatment durations are a viable alternative to lethal ones for N. perurans (2–4 hr). Under in vitro conditions, gill‐isolated N. perurans attached to plastic substrate in sea water lifted off after ≥2 min in freshwater, but survival was not impacted until 60 min. In an in vivo experiment, AGD‐affected Atlantic salmon Salmo salar subjected daily to 30 min (sublethal to N. perurans) and 120 min (lethal to N. perurans) freshwater treatments for 6 days consistently reduced N. perurans cell numbers on gills (based on qPCR analysis) compared to daily 3 min freshwater or seawater treatments for 6 days. Our results suggest that targeting cell detachment rather than cell death with repeated freshwater treatments of shorter duration than typical baths could be used in AGD management. However, the consequences of modifying the intensity of freshwater treatment regimes on freshwater tolerance evolution in N. perurans populations require careful consideration. 相似文献
10.
Kim SP Kang MY Kim JH Nam SH Friedman M 《Journal of agricultural and food chemistry》2011,59(18):9861-9869
We investigated antitumor effects of the following four extracts of freeze-dried Hericium erinaceus mushrooms in Balb/c mice intracutaneously transplanted on the backs with CT-26 colon cancer cells: HWE, hot water extraction by boiling in water for 3 h; MWE, microwaving in 50% ethanol/water at 60 W for 3 min; and ACE and AKE, boiling in 1% HCl or 3% NaOH for 2 h. HWE and MWE with a higher content of β-glucans, determined by an assay kit, than ACE and MKE were active in all bioassays. Gas chromatography/mass spectrometry analyses showed the presence of 40, 27, 16, and 13 compounds, respectively, in the four extracts. Daily intraperitoneal (ip) injections of HWE and MWE for 2 weeks significantly reduced tumor weights by 38 and 41%. Tumor regressions were associated with changes in the following cancer biomarkers as compared to phosphate buffer (PBS)-treated control mice: 2.7- and 2.4-fold increases in cytolytic activity of splenic natural killer (NK) cells; restored nitric oxide production and phagocytosis in peritoneal macrophages to 95-98% of normal levels; ~2-fold increase in released pro-inflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 from macrophages; and ~56 and ~60% reductions in the number of blood vessels inside the tumor. The pro-angiogenic factors vascular endothelial growth factor (VEGF), cyclooxygenase 2 (COX-2), and 5-lipoxygenase (5-LOX) were also significantly reduced in mRNA and protein expression by tumor genes. Enzyme-linked immunosorbent assay of tumor cells confirmed reduced expression of COX-2 and 5-LOX (32 and 31%). Reduced COX-2 and 5-LOX expression down-regulated VEGF expression, resulting in inhibition of neo-angiogenesis inside the tumors. The results indicate that induction of NK activity, activation of macrophages, and inhibition of angiogenesis all contribute to the mechanism of reduction of tumor size. 相似文献