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Magdalena Pacwa-Płociniczak Tomasz Płociniczak Dan Yu Jukka M. Kurola Aki Sinkkonen Zofia Piotrowska-Seget Martin Romantschuk 《Water, air, and soil pollution》2018,229(1):13
In this study, we analysed the impact of heavy metals and plant rhizodeposition on the structure of indigenous microbial communities in rhizosphere and bulk soil that had been exposed to heavy metals for more than 150 years. Samples of the rhizosphere of Silene vulgaris and non-rhizosphere soils 250 and 450 m from the source of emission that had different metal concentrations were collected for analyses. The results showed that soils were collected 250 m from the smelter had a higher number of Cd-resistant CFU compared with the samples that were collected from 450 m, but no significant differences were observed in the number of total and oligotrophic CFU or the equivalent cell numbers between rhizosphere and non-rhizosphere soils that were taken 250 and 450 m from the emitter. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis of the denaturing gradient gel electrophoresis (DGGE) profiles, as well as a cluster analysis that was generated on the phospholipid fatty acid (PLFA) profiles, showed that the bacterial community structure of rhizosphere soils depended more on the plant than on the distance and metal concentrations. The sequencing of the 16S rDNA fragments that were excised from the DGGE gel revealed representatives of the phyla Bacteroidetes, Acidobacteria, Gemmatimonadetes, Actinobacteria and Betaproteobacteria in the analysed soil with a predominance of the first three groups. The obtained results demonstrated that the presence of S. vulgaris did not affect the number of CFUs, except for those of Cd-resistant bacteria. However, the presence of S. vulgaris altered the soil bacterial community structure, regardless of the sampling site, which supported the thesis that plants have a higher impact on soil microbial community than metal contamination. 相似文献
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Successful forest restoration requires planting quality seedlings with optimal growth potential. Thus, nurseries need to produce seedlings with plant attributes that favor the best chance of successful establishment once they are field planted. From the mid-twentieth century on, research foresters have critically examined plant attributes that confer improved seedling growth under various restoration site conditions. This review examines the value of commonly measured seedling quality attributes (i.e., height, diameter, root mass, shoot-to-root ratio, drought resistance, freezing tolerance, nutrient status, root growth potential, and root electrolyte leakage) that have been recognized as important in explaining why seedlings with improved attributes have better growth after planting. Seedlings with plant attributes that fall within the appropriate range of values can increase the speed with which they overcome planting stress, initiate growth, and become “coupled” to the forest restoration site, thereby ensuring successful seedling establishment. Although planting high quality seedlings does not guarantee successful seedling establishment, it increases chances for successful establishment and growth. 相似文献
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Urbanozems (Urbic Technosols) contaminated by heavy metals and polychlorbiphenyls (Urbic Technosols Toxic) and intruzems (Urbic Technosols Toxic) were studied in Moscow; additionally, we studied recreazems (Urbic Technosols Thaptohumic) and culturozems (Urbic Technosols Pantohumic) on the territory of the Botanical Garden of Moscow State University (Aptekarskii Ogorod, the Apothecaries’ Garden). In the soils contaminated with heavy metals and oil products, the number of viable cells of bacteria decreased, whereas the content of filterable forms of bacteria increased. The taxonomic structure of saprotrophic bacterial complexes in contaminated urban soils was transformed towards an increase in the diversity of bacterial taxa atypical of natural undisturbed soils. Rhodococci (Rhodococcus genus) predominated in the soils contaminated with oil and polychlorbiphenyls, enterobacteria (Escherichia, Enterobacter, and Klebsiella genera) predominated in the soils contaminated with municipal wastes, and Arthrobacter genus was dominant in the soils contaminated with cement dust. Soils of both Botanical Gardens of Moscow State University were characterized by the high population density and specific distribution of bacteria in the profile; the structure of their saprotrophic bacterial complex had some similarity with that in the soils of more southern regions. The obtained data on the bacterial diversity of urban soils attest to considerable transformation of bacterial communities both in the contaminated urban soils and in the soils of botanical gardens. 相似文献
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Processes of initial soil formation were studied on long-term monitoring plots on dump rocks of quarry no. 3 of the Phosphorite production company in Kingisepp district of Leningrad oblast. Observations were performed in 1998, 2004, and 2014. It was shown that vegetation succession on the plots proceeds relatively quickly, and that the species composition of phytocenoses formed is typical of the areas with soddy-calcareous soils. Soil development proved to be correlated with the development of vegetation. Maximum changes in soil characteristics were observed with an increase in the density of forest vegetation and a decrease in the role of herbs. The molecular composition of humic acids in the studied soils remained stable; in particular, the ratio of aliphatic to alkyl aromatic fragments was virtually constant. This phenomenon could be due to the great amount of aliphatic components in the falloff of coniferous species subjected to humification. 相似文献
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H.-J. Krauthausen Gabi Hörner Silke Zimmermann R. T. Voegele F. Brändle 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(2):275-286
Aphanomyces euteiches Drechsler is an oomycete pathogen of leguminous crops that causes root rot, a severe disease of pea (Pisum sativum L.) worldwide. An improved understanding of the genetic structure of A. euteiches populations would increase knowledge of pathogen evolution and assist in the design of strategies to develop pea cultivars and germplasm with stable disease resistance. Twenty six primers pairs were used to amplify Sequence Related Amplified Polymorphisms (SRAP) among 49 A. euteiches isolates sampled from pea. A total of 190 polymorphic SRAP bands were generated, of which 82 were polymorphic between all the A. euteiches isolates. The percentage of polymorphic bands per primer pair ranged from 22 to 75%. According to the PIC value estimated for each marker, 60% of the SRAP markers were highly to reasonably informative (PIC > 0.25). Genetic structure of A. euteiches populations sampled in different American and French locations showed low to high genetic diversity within populations. The largest variation occurred within countries, with a total estimated genetic diversity of 0.477 and 0.172 for American and French populations, respectively. This was particularly evident from a principal component analysis (PCA) and a Minimum Spanning Networks (MSN) based on genetic profiles of isolates, which generated two different clusters, one corresponding to the French isolates and four American isolates (MV1, MV5, MV7, Ath3), and the other to American isolates. A. euteiches populations from cultivated pea in France appeared as a single unstructured population, whereas American isolates of A. euteiches diverged into three different populations. 相似文献
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Anna Berlin A.-C. Wallenhammar B. Andersson 《European journal of plant pathology / European Foundation for Plant Pathology》2018,151(4):901-917
The fungus Puccinia coronata Corda. is the causal agent of crown rust on oats (Avena sativa) and grasses and the disease is a major problem in oat production causing devastating yield losses. The population biology of P. coronata in oat fields and on the aecial host in central Sweden was studied to get a deeper understanding of the role of the aecial hosts in the epidemiology of the disease. Samples were collected from the aecial hosts common buckthorn (Rhamnus cathartica) and alder buckthorn (Frangula alnus), and three adjacent spring oat (Avena sativa) fields. Microsatellite markers were used to evaluate the relationships between populations sampled from the different hosts. According to our results F. alnus can be excluded as a part of the oat crown rust disease cycle. The results further show that samples collected from the aecial host were genetically separate from the population sampled in adjacent oat fields. Concurrently, the genotypic variation of P. coronata observed within oat fields was high. No population differentiation was observed within or between samples collected from different fields within the region, suggesting that airborne spores from other than the sampled specimens of the aecial hosts were contributing to the genetic diversity of P. coronata f. sp. avenae in the selected oat fields. 相似文献