Evaluation of genetic variation among wild rice populations in Cambodia

A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources.     

Finding a needle in the haystack: Regional analysis of suitable Eld’s deer (Cervus eldi) forest in Southeast Asia

For species whose decline preceded the modern era and whose distribution is in the developing world, it is difficult to map suitable habitat across its former range. Eld’s deer (Cervus eldi) is an endangered cervid whose range across Southeast Asia was reduced during the last century to disjoint populations in Myanmar and Cambodia. We used ecological data from the present populations to determine landscape and habitat parameters that would help us predict the occurrence of the species in forests not yet surveyed. The suitable-forest GIS model was created using four readily available datasets for elevation, forest type, canopy closure, and human density. Comparison of the GIS model with 24 verified sightings of Eld’s deer during recent large mammal surveys in Cambodia, found 22 sightings (92%) within predicted suitable forest. Use the suitable-forest GIS model to survey a province in southern Lao People’s Democratic Republic, located a single, previously unreported population from 9 patches surveyed. In a separate analysis, a logistic regression model to predict Eld’s deer habitat in Northern Cambodia found percent tree cover, presence of wetlands, and distance to villages as the best predictors of deer, similar to variables used in the GIS model, with the exception of the importance of wetlands. Using mean annual rainfall to rank suitable-forest patches identified in the GIS model indicated dry dipterocarp forests in Northeastern Cambodia and Northern Myanmar have the highest potential to conserve eld’s deer. Examination of the suitable-forest GIS map and current protected areas indicated only Cambodia, with 11% suitable forest protected, has placed sufficient dry dipterocarp forest under protected status. Other Southeast Asia countries have not recognized dry dipterocarp forest as a significant ecotype worthy of conservation status.     

Crop rotation sequencing to minimize yield losses of summer-irrigated lowland rice in Myanmar caused by the rice root-knot nematode Meloidogyne graminicola

Abstract

The effect of different rice-based crop rotation sequences on the population densities of Meloidogyne graminicola and on the yield of rice was evaluated in a microplot experiment under lowland rice field conditions. Ten treatments of cropping sequences were grown in four successive growing seasons: continuous growing of the susceptible rice cultivar Thihtatyin (rice-rice-rice-rice), four treatments of 1- season crop rotation sequences (rice-rice-chickpea-rice, rice-rice-black gram-rice, rice-rice-soybean-rice, rice-rice-cowpea-rice), and five treatments of 2-season crop rotation sequences (groundnut-rice-chickpea-rice, green gram-rice-black gram-rice, cowpea-rice-soybean-rice, sesame-rice-cowpea-rice, sunflower-rice-sesame-rice). Population densities of M. graminicola recovered from the rice plants and the nematode multiplication factors (Mf) in the soil in the continuous rice cropping sequence (rice-rice-rice-rice) was the highest among the ten cropping sequences. Lowest nematode Mf in the soil was observed in the 2-season crop rotation sequence sunflower-rice-sesame-rice and the highest was observed in the 1-season crop rotation sequence rice-rice-cowpea-rice among the nine crop rotation sequences. Highest rice yield was obtained in the 2-season crop rotation sequences green gram-rice-black gram-rice, sesame-rice-cowpea-rice and sunflower-rice-sesame-rice, which were about 2 times higher compared with the 1-season crop rotation sequences and about 3 times higher compared with the continuous rice cropping sequence.     

Effect of soybean husk supplementation on the fecal fermentation metabolites and microbiota of dogs

In vitro fermentation and in vivo feeding experiments were conducted to characterize the effects of soybean (Glycine max) husk on the fecal fermentation metabolites and microbiota of dogs. An in vitro fermentation study using feces from three Toy Poodle dogs (6.5 ± 3.5 months in age and 2.9 ± 0.4 kg in body weight) revealed that the fecal inoculum was able to ferment soybean husk (supplemented at 0.01 g/mL culture) and increased levels of short chain fatty acids (SCFA) and Bifidobacterium, irrespective of pre‐digestion of the husk by pepsin and pancreatin. In a feeding experiment, four Shiba dogs (7–48 months in age and 7.5 ± 1.7 kg in body weight) fed a commercial diet supplemented with 5.6% soybean husk showed an increase in SCFA, such as acetate and butyrate, and lactate, and a decrease in indole and skatole in the feces compared to those fed a 5.6% cellulose diet. Real‐time PCR assay showed that soybean husk supplementation stimulated the growth of lactobacilli, Clostridium cluster IV including Faecalibacterium prausnitzii, Clostridium cluster XIVa, Bacteroides‐Prevotella‐Porphyromonas group but inhibited the growth of Clostridium cluster XI. Both in vitro and in vivo experiments indicated that soybean husk supplementation improves gastrointestinal health through optimization of beneficial organic acid production and increase of beneficial bacteria. Therefore, soybean husk is suggested to be applicable as a functional fiber in the formulation of canine diets.     

Sulphur supply impairs spread of Verticillium dahliae in tomato

Vascular wilt caused by the soil-borne fungus Verticillium dahliae is a major yield and quality-limiting disease across a broad spectrum of crop plants worldwide. Sulphur-enhanced plant defence mechanisms provide an opportunity to effectively and environmentally safely constrain the wilt disease levels in planta. To evaluate the influence of sulphur nutrition on the protective potential of these mechanisms, two near-isogenic tomato genotypes differing in fungal susceptibility, were treated with low or supra-optimal sulphur supply. Microscopic analysis revealed a significant sulphur-induced decrease in the amount of infected vascular cells in both genotypes. However, plant shoot and severely pathogen-affected root growth did not display this distinct alleviating influence of sulphur nutrition. Rates of leaf photosynthesis were impeded by Verticillium dahliae infection in both genotypes especially under low sulphur nutrition. However, assimilate transport rates in the phloem sap were enhanced by fungal infection more in the resistant genotype and under high sulphur nutrition suggesting a stronger sink for assimilates in infected plant tissues possibly involved in sugar-induced defence. A SYBR Green-based absolute quantitative Real-Time assay using a species-specific primer was developed which sensitively reflected sulphur nutrition-dependent changes in fungal colonization patterns. High sulphur nutrition significantly reduced fungal spread in the stem in both tomato genotypes. Concentrations of selected sulphur-containing metabolites revealed an increase of the major anti-oxidative redox buffer glutathione under high sulphur nutrition in response to fungal colonization. Our study demonstrates the existence of sulphur nutrition-enhanced resistance of tomato against Verticillium dahliae mediated by sulphur-containing defence compounds.     

The impact of urban expansion on the regional environment in Myanmar: a case study of two capital cities

Context

This study examines the spatio-temporal patterns of urban expansion for Yangon and Nay Pyi Taw, the former and new national capitals of Myanmar, and its impact on the regional environment between 2000 and 2013.

Objectives

The objective is to examine different driving forces of urban expansion for Yangon and Nay Pyi Taw, and their environmental consequences during Myanmar’s transitional economy.

Methods

Classified time-series Landsat images are used to evaluate urban expansion processes. Environmental parameters being evaluated in this study include five sets of remotely sensed MODIS land products that are land surface temperature (LST), percent tree cover (PTC), evapotranspiration (ET), terrestrial ecosystem net primary productivity (NPP), and aerosol optical depth (AOD). A time-series trend analysis technique is used to examine the environmental consequences.

Results

The built-up areas in Nay Pyi Taw and Yangon exhibit exponential and polynomial increase, respectively. A 1% increase of built-up area could potentially cause an increase of daytime LST of 0.7 °C, a PTC loss of 2.3%, a decrease in NPP of 34.3 kg/m², and an ET decrease of 42.2 mm for Yangon. Similarly, for Nay Pyi Taw, a 1% increase in built-up area could potentially cause a daytime LST increase of 0.3 °C, a nighttime LST increase of 0.06 °C, a PTC loss of 2.5%, a decrease in NPP of 15.1 kg/m², and a decrease of 19.2 mm ET. No significant change was observed for AOD for either city.

Conclusions

Both cities have experienced extensive urban expansion but with different spatial and temporal characteristics, and their effects on the regional environment are different. Urban expansion of Nay Pyi Taw mainly was government-induced municipal infrastructure development. Yangon’s expansion is mainly caused by population pressure and migration from rural areas. The urban expansion in Yangon was mainly due to reconstruction and renovation, as well as infill development during the study period.

     

Micro-scale urban surface temperatures are related to land-cover features and residential heat related health impacts in Phoenix,AZ USA

Context

With rapidly expanding urban regions, the effects of land cover changes on urban surface temperatures and the consequences of these changes for human health are becoming progressively larger problems.

Objectives

We investigated residential parcel and neighborhood scale variations in urban land surface temperature, land cover, and residents’ perceptions of landscapes and heat illnesses in the subtropical desert city of Phoenix, AZ USA.

Methods

We conducted an airborne imaging campaign that acquired high resolution urban land surface temperature data (7 m/pixel) during the day and night. We performed a geographic overlay of these data with high resolution land cover maps, parcel boundaries, neighborhood boundaries, and a household survey.

Results

Land cover composition, including percentages of vegetated, building, and road areas, and values for NDVI, and albedo, was correlated with residential parcel surface temperatures and the effects differed between day and night. Vegetation was more effective at cooling hotter neighborhoods. We found consistencies between heat risk factors in neighborhood environments and residents’ perceptions of these factors. Symptoms of heat-related illness were correlated with parcel scale surface temperature patterns during the daytime but no corresponding relationship was observed with nighttime surface temperatures.

Conclusions

Residents’ experiences of heat vulnerability were related to the daytime land surface thermal environment, which is influenced by micro-scale variation in land cover composition. These results provide a first look at parcel-scale causes and consequences of urban surface temperature variation and provide a critically needed perspective on heat vulnerability assessment studies conducted at much coarser scales.

     

Fruit and vegetable consumption and self-reported functional health in men and women in the European Prospective Investigation into Cancer-Norfolk (EPIC-Norfolk): a population-based cross-sectional study

OBJECTIVES: To investigate the association between fruit and vegetable consumption and self-reported physical and mental functional health measured by an anglicised short-form 36-item questionnaire (UK SF-36). DESIGN: Population-based cross-sectional study. SETTING: General community in Norfolk, UK. SUBJECTS: A total of 16,792 men and women aged 40-79 years recruited from general practice population registers as part of the European Prospective Investigation into Cancer (EPIC)-Norfolk study, who completed food-frequency questionnaires in 1993-1997 and Health and Life Experiences Questionnaires 18 months later, were enrolled in the study. RESULTS: Mean SF-36 physical component summary scores increased significantly with increasing total fruit and vegetable consumption in both men and women (P < 0.0001 for trend). Men and women in the top quartile of consumption compared with the bottom quartile had a significantly higher likelihood of reporting good physical health (defined as a score > or = 55); odds ratio (OR) 1.30, 95% confidence interval (CI) 1.11-1.53 for men and OR 1.28, 95% CI 1.11-1.48 for women, after controlling for age, body mass index, smoking, education, social class, prevalent illness and total energy intake. Exclusion of current smokers and people with prevalent illness did not alter the associations. CONCLUSION: Higher fruit and vegetable consumption is associated with better self-reported physical functional health within a general population. Increasing daily intake by two portions of fruit and vegetables was associated with an 11% higher likelihood of good functional health. Since the current average consumption of fruit and vegetables in the UK is about three portions, the recommended 'five a day' strategy may have additional benefit for functional as well as other health outcomes in the population.     

Habitual fish consumption and risk of incident stroke: the European Prospective Investigation into Cancer (EPIC)-Norfolk prospective population study

OBJECTIVES: To examine the association between fish consumption and stroke risk. DESIGN: Prospective population cohort study.Setting: Norfolk, UK cohort of the European Prospective Investigation into Cancer (EPIC-Norfolk). SUBJECTS: Subjects were 24 312 men and women aged 40-79 years who had no previous history of stroke at baseline.Methods: Fish consumption was assessed using a food-frequency questionnaire at baseline in 1993-1997 and stroke incidence ascertained to 2004. RESULTS: A total of 421 incident strokes were identified (mean follow-up=8.5 years, total person-years=209 238). There were no significant relationships between total fish, shellfish or fish roe consumption and risk of stroke in men and women after adjusting for age, systolic blood pressure, body mass index, smoking, cholesterol, diabetes, physical activity, alcohol consumption, fish oil supplement use and total energy intake using Cox regression analyses. Oily fish consumption was significantly lower in women who subsequently had a stroke (odds ratio (OR) for consumers vs. non-consumers=0.69, 95% confidence interval (CI) 0.51-0.94, P=0.02). The trend in men was similar but not significant (OR for consumers vs. non-consumers=0.88, 95% CI 0.65-1.19, P=0.41). CONCLUSIONS: There was no consistent relationship between fish consumption and stroke in this British population. Inconsistencies in the observed health effects of fish consumption in different populations may reflect different patterns and type of fish consumed and preparation methods.