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1.
Fariba Ghaderi Bahram Sharifnabi Mohammad Javan-Nikkhah Patrick C. Brunner Bruce A. McDonald 《Plant pathology》2020,69(8):1482-1491
The centre of origin of the globally distributed wheat pathogen Parastagonospora nodorum has remained uncertain because only a small number of isolates from the Fertile Crescent were included in earlier population genetic and phylogeographic studies. We isolated and genetically analysed 193 P. nodorum strains from three naturally infected wheat fields distributed across Iran using 11 neutral microsatellite loci. Compared to previous studies that included populations from North America, Europe, Africa, Australia, and China, the populations from Iran had the highest genetic diversity globally and also exhibited greater population structure over smaller spatial scales, patterns typically associated with the centre of origin of a species. Genes encoding the necrotrophic effectors SnToxA, SnTox1, and SnTox3 were found at a high frequency in the Iranian population. By sequencing 96 randomly chosen Iranian strains, we detected new alleles for all three effector genes. Analysis of allele diversity showed that all three effector genes had higher diversity in Iran than in any population included in previous studies, with Iran acting as a hub for the effector diversity that was found in other global populations. Taken together, these findings support the hypothesis that P. nodorum originated either within or nearby the Fertile Crescent with a genome that already encoded all three necrotrophic effectors during its emergence as a pathogen on wheat. Our findings also suggest that P. nodorum was the original source of the ToxA genes discovered in the wheat pathogens Phaeosphaeria avenaria f. sp. tritici 1, Pyrenophora tritici-repentis, and Bipolaris sorokiniana. 相似文献
2.
Jeannette S. Bayuelo-Jiménez Takashi Muraoka Eulogio de la Cruz-Torres Esperanza Quintero-Ponce Lydia C. Paredes-Gutiérrez Mohammad Zaman 《Soil Use and Management》2020,36(2):240-249
Land-use change from forest to agriculture in the volcanic ash-derived soils of Mexico has increased over recent decades. It is likely that land uses and management practices, particularly fertilizer use have affected phosphorus (P) distribution and availability. The objective of this study was to evaluate the effects of land-use types (native forest and maize mono-cropping), and the related P addition, on the forms and distribution of soil P and their isotopic exchangeability. An Andisol, sampled from a cropping site, along with the contiguous area under native forest was treated with 32P-labelled potassium phosphate (KH232PO4). The soil samples were extracted after incubation times of 7, 21, 35 and 49 days. Phosphorus content and 32P recovery in fractions sequentially extracted were assessed for each incubation time. Total soil P was dominated by inorganic fractions (79 to 86%) in both land-use types. Resin-Pi, bicarbonate extractable inorganic P (Bic-Pi) and sodium hydroxide extractable inorganic P (NaOH0.1-Pi) were all raised with P addition. However, the proportion of organic P fraction was reduced under cropped soil. The recovery of 32P in soils with P addition indicates that resin-Pi, Bic-Pi and NaOH0.1-Pi comprised nearly all the exchangeable P. In native soils with no P addition, more than 19% of the 32P was recovered in Bic-Po and NaOH0.1-Po forms. This finding indicates that organic P cycling is crucial when soil Pi reserves are presented in an inadequate amount. Ecologically based management has to be designed for replenishment and succeeding maintenance of soil organic P compounds to increase sustainable agricultural production. 相似文献
3.
Soheila Espanani Mohammad Mahdi Majidi Ghodratollah Saeidi Hossein Alaei Vahid Rezaei 《Plant Breeding》2019,138(6):846-861
Possibility of using interspecific hybridization to increase genetic diversity of drought tolerance in safflower (Carthamus tinctorius L.) and effectiveness of different selection methods is poorly understood. The main objectives of this study were to evaluate the effectiveness of (a) interspecific hybridization to expand genetic diversity in safflower for agronomic traits and drought tolerance in advance generations and (b) different selection methods (direct vs. indirect) for improving seed yield. Interspecific hybridizations were performed between species of C. tinctorius × C. palaestinus (TP), C. palaestinus × C. oxyacantha (PO) and C. tinctorius × C. oxyacantha (TO). Based on the field screening of genotypes in F3 generation, four groups of selected genotypes (derived from combination of direct and indirect selection under both drought and normal environments along with selection based on drought tolerance index (STI)) were evaluated in F4 and F5 generations. Indirect selection for seed yield was significantly more efficient than direct selection. Selection in stress environment resulted to more efficiency than selection in normal environment. No significant difference was observed between direct selection and selection based on STI. The result showed that TP was the best population to combine high seed yield and drought tolerance in safflower. 相似文献
4.
5.
Majid Salehi Ahmad Vaez Mahdi Naseri-Nosar Saeed Farzamfar Arman Ai Jafar Ai Shima Tavakol Mehrdad Khakbiz Somayeh Ebrahimi-Barough 《Fibers and Polymers》2018,19(1):125-134
In the present study, naringin, a flavonoid isolated from the grape and citrus fruit species, was incorporated with poly(ε-caprolactone)/gelatin composite mats in order to develop a potential wound dressing. The composite mats were prepared by electrospinning of poly(ε-caprolactone)/gelatin (1:1 (w/w)) solution incorporated with 1.50 %, 3 % and 6 % (w/w) of naringin. The electrospun mats were evaluated regarding their morphology, contact angle, water-uptake capacity, water vapor transmission rate, tensile properties, drug release, cellular response and in vivo wound healing activity. The study showed that after 2 weeks, the full-thickness excisional wounds of Wistar rats treated with the naringin-loaded dressings achieved a wound closure of higher than 94 % and the dressing containing 6 % (w/w) naringin had almost 100 % wound closure. The sterile gauze, as the control group, showed nearly 86 % of wound closure after this period of time. Our results provided evidence that supports the possible applicability of naringin-loaded wound dressing for successful wound treatment. 相似文献
6.
Saddleback-like syndrome is one of the most frequently observed malformations in hatchery-reared red spotted grouper Epinephelus akaara. Saddleback-like syndrome is characterized by a V-shaped dorsal-profile depression behind the head region accompanied by deformities or misalignments of the first to fourth neural spines on the vertebral column and the first to fourth pterygiophores of the dorsal fin. Abnormal swim bladder inflation in several finfish species leads to malformations, particularly of the vertebral column. The present study examined the influence of swim bladder inflation on saddleback-like syndrome in hatchery-reared red spotted grouper. The incidence of saddleback-like syndrome increased as swim bladder inflation rate increased. Additionally, the incidence of saddleback-like syndrome among juveniles with inflated swim bladders was significantly higher than that among juveniles with uninflated swim bladders. The larval swim bladder is located just below the first to fifth vertebrae and the first to fifth dorsal spines, where saddleback-like syndrome occurs. Our results suggest that an inflated swim bladder increases the risk of saddleback-like syndrome in hatchery-reared red spotted grouper. 相似文献
7.
R. C. Silva S. Liebel H. H. P. de Oliveira W. A. Ramsdorf J. R. E. Garcia S. M. F. O. Azevedo V. F. Magalhães C. A. Oliveira Ribeiro F. Filipak Neto 《Fish physiology and biochemistry》2017,43(5):1237-1244
Cylindrospermopsin (CYN) is a cyanotoxin that is cytotoxic to a wide variety of cells, particularly to the hepatocytes. In this study, the toxic effects of purified CYN were investigated in primary cultured hepatocytes of Neotropical fish Hoplias malabaricus. After isolation, attachment, and recovery for 72 h, the cells were exposed for 72 h to 0, 0.1, 1.0, 10, and 100 μg l?1 of CYN. Then, cell viability and a set of oxidative stress biomarker responses were determined. Catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase, and glutathione S-transferase activities were not affected by exposure to CYN. Concentration-dependent decrease of glutathione reductase activity occurred for most CYN-exposed groups, whereas non-protein thiol content increased only for the highest CYN concentration. Lipid peroxidation, protein carbonylation, and DNA damage levels were not altered, but reactive oxygen species levels increased in the cells exposed to the highest concentration of CYN. Cell viability decreased in all the groups exposed to CYN. Thus, CYN may cause a slight change in redox balance, but it is not the main cause of cell death in H. malabaricus hepatocytes. 相似文献
8.
Issa Mohammadpourfard Mir Mohammad Shamloo Anbardan Navid Eshghi Mahdi Jahanbakhsh Arash Akbarzadeh Saeed Ghorbani Bejandi 《Journal Of Aquatic Food Product Technology》2017,26(6):756-768
Today, due to the rapid spoilage of fish, the use of natural preservatives is a priority over those of synthetic varieties. Also, the natural antimicrobial effects of essential oils can help to increase shelf life. There are very few studies concerning the use of essential oils in this regard. In the present study, the antibacterial effect of chitosan coated with Heracleum persicum oil was investigated on the quality of rainbow trout. The control and the coated fish samples were analyzed periodically by generalized estimating equation (GEE) for total viable count (TVC), psychrophilic bacteria, total volatile base nitrogen (TVB-N), polyunsaturated fatty acids (PUFA), texture, taste, odor, and overall acceptance parameters. The results showed that by increasing the amount of chitosan, TVC, psychrophilic bacteria, and TVB-N decreased while PUFA, texture, taste, odor, and overall acceptance parameters increased. 相似文献
9.
This paper explores the plasticity of sexually dimorphic characters in subadult female Brevimyrus niger, an African weakly electric mormyrid species. Thirty-five fish were exposed in a staggered fashion (five fish a week) to aromatizable 17α-methyltestosterone over a period of 7 weeks; 18 fish served as untreated controls. 17α-MT induced precocious vitellogenesis that mirrored the natural maturational process during seasonal ovarian recrudescence. At the same time, 17α-MT exposure resulted in complete masculinization of the females’ anal fin support structure normally observed during rainy season in adult males. We discuss possible hormonal mechanisms acting along the brain-pituitary-gonad axis that would explain the occurrence of precocious vitellogenesis and the male-typical transformation of the female’s anal fin ray bases. Our findings are relevant to commercial aquaculture as the use of 17α-MT in fish hatcheries can pose serious environmental issues. 相似文献
10.
Growth and economic analysis of freshwater prawn,Macrobrachium rosenbergii (de Man), produced with feeds substituting sunflower cake for fish meal,soya bean meal and mustard oil cake 下载免费PDF全文
A feeding trial with Macrobrachium rosenbergii was carried out to test the effectiveness of sunflower cake (SFC) as a replacement for conventional protein sources. Four isonitrogenous (~30% crude protein) and isocaloric (388–402 kcal) diets were formulated. Diet‐1 was a control made with conventional ingredients including fish meal, soya bean meal and mustard oil cake. Diet‐2, Diet‐3 and Diet‐4 were made with 30%, 40% and 50% SFC, respectively, replacing the usual protein sources used in Diet‐1. After 100 days, diets with SFC inclusion showed favourable growth and economic performance trends compared to control diet‐1, but without significant differences (p > .05). Better production (572.89 ± 62.99 kg/ha) was observed with Diet‐4 followed by Diet‐2, Diet‐3 and Diet‐1. Diet‐4 revealed the best feed conversion ratio (2.56 ± 0.24), specific growth rate (1.18 ± 0.05) and protein efficiency ratio (1.14 ± 0.10) among these diets. Whole carcass composition analysis revealed highest crude protein (18.19%) and lipid (4.43%) content in prawns fed Diet‐3 followed by Diet‐4. Highest total income, gross margin and benefit cost ratio (BCR) per hectare from prawns were US$5,759, US$2,623 and 1.84, respectively, found in Diet‐4. This study demonstrates that 30%–50% inclusion of SFC has favourable impacts on productivity and profitability compared with controls, with 50% SFC inclusion as the most cost‐effective diet for prawns without apparent compromise in growth, flesh quality or economics. The performances of the diets suggest that SFC may be a suitable substitute for fishmeal, soya bean meal and mustard oil cake in prawn feed. The effectiveness of higher SFC concentrations should also be investigated. 相似文献