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排序方式: 共有226条查询结果,搜索用时 31 毫秒
1.
Serologic and fecal markers to predict response to induction therapy in dogs with idiopathic inflammatory bowel disease 下载免费PDF全文
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Mercedes Fernandez-Moreno Alberto Arias-Perez Ruth Freire & Josefina Méndez 《Aquaculture Research》2008,39(5):474-481
Aequipecten opercularis (Queen scallop) and Mimachlamys (Chlamys) varia (Black scallop) are important natural resources occurring in Atlantic and Mediterranean coasts. To develop an optimal sustainable exploitation plan, it is important to study the genetic structure of the different populations. In this study, we used polymerase chain reaction‐restriction fragment length polymorphisms for the determination of the genetic variation and population structure of these two species in different localities. Ten composite haplotypes were generated for A. opercularis and 15 haplotypes for M. varia. Of these, six and four were unique respectively. The analysis of the distribution of the different haplotypes between the localities showed no clear evidence of subdivision in A. opercularis, while in M. varia the results indicated that the two localities analysed should be managed as separate stocks. 相似文献
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Raico E Laria Lamela Yanis Cruz Quintana Raquel Silveira Coffigny Mercedes Martínez Norma González Herrate 《Aquaculture Research》2008,39(12):1316-1321
Formalin is used in shrimp industry to eliminate ectoparasites and as a disinfectant. However, the effect of formalin on shrimp's defense mechanism is unknown. Several biomarkers were used to assess the immune response of juveniles of the shrimp Litopenaeus schmitti exposed to different concentrations of formalin (10, 25 and 50 mg L?1) during 24 and 48 h. Formalin concentrations of 25 and 50 mg L?1 produced a significant reduction in the total haemocyte count at both 24 and 48 h. Peroxidase and phenoloxidase activity did not show variations. The total protein concentration in haemolymph of shrimps was not significantly different between experimental groups. Histological analysis showed hyperplasia in the gills of animals exposed at 10 mg L?1 for 24 h; however, the exposure at 25 and 50 mg L?1 caused necrosis in gills, hepathopancreas and the antennal gland. 相似文献
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Inmaculada Moreno Julio Álvarez Nerea García Santiago de la Fuente Irene Martínez Eloy Marino Alfredo Toraño Joaquin Goyache Felipe Vilas Lucas Domínguez Mercedes Domínguez 《Veterinary parasitology》2014,199(3-4):264-267
An outbreak of human leishmaniosis was confirmed in the southwest of the province of Madrid, Spain, between July 2009 and December 2012. Incidence of Leishmania infection in dogs was unchanged in this period, prompting a search for alternative sylvatic infection reservoirs. We evaluated exposure to Leishmania in serum samples from animals in the area with an indirect immunofluorescence test (IFAT). Using promastigotes from six culture passages and a 1/25 threshold titer, we found anti-Leishmania infantum seroreactivity in 9.3% of cats (4 of 43), 45.7% of rabbits (16/35) and 74.1% of hares (63/85). Use of promastigotes from >10 in vitro passages resulted in a notably IFAT lower titer, suggesting antigenic changes during extended culture. Postmortem inspection of seropositive animals showed no clinical signs of infection. The results clearly suggest that asymptomatic hares were the main reservoir in the outbreak, and corroborate IFAT as a sensitive serological surveillance method to detect such cryptic Leishmania infections. 相似文献
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Marta González-Warleta José Antonio Castro-Hermida Javier Regidor-Cerrillo Julio Benavides Gema álvarez-García Miguel Fuertes Luis Miguel Ortega-Mora Mercedes Mezo 《Veterinary research》2014,45(1)
Neospora caninum has been detected only sporadically in cases of ovine abortion, and it has therefore traditionally been considered as an unimportant parasite in small ruminants. This study was carried out with the aim of identifying the pathogen causing serious reproductive problems on a commercial sheep farm. Sera from all rams and ewes tested negative for antibodies against Border disease virus, Schmallenberg virus and Coxiella burnetii, and infections by these agents were therefore ruled out. Nevertheless, seropositivity to N. caninum and/or Toxoplasma gondii was detected, although the seroprevalence was higher in the case of N. caninum. The percentage of lambings and the number of lambs per dam were significantly lower in ewes that were seropositive to N. caninum while no effect on these parameters was detected in ewes that were seropositive to T. gondii. There was also no evidence of infection by T. gondii in the foetal/lamb tissues analyzed by PCR and/or immunohistopathological techniques. On the contrary, the DNA of N. caninum was detected in 13 out of 14 foetuses/lambs descendant from dams seropositive to this parasite. Characteristic lesions caused by N. caninum and/or its antigen were also detected. Genotyping of the N. caninum DNA revealed only two closely related microsatellite multilocus genotypes. The results clearly demonstrate that infection by N. caninum was the cause of the low reproductive performance of this sheep flock. 相似文献
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Mercedes Uscola Juan A. Oliet Pedro Villar-Salvador Eugenio Díaz-Pinés Douglass F. Jacobs 《European Journal of Forest Research》2014,133(2):235-246
Most studies examining inorganic N form effects on growth and nutrition of forest trees have been conducted on single species from boreal or temperate environments, while comparative studies with species from other biomes are scarce. We evaluated the response of two Mediterranean trees of contrasting ecology, Quercus ilex L. and Pinus halepensis Mill., to cultivation with distinct inorganic N forms. Seedlings were fertilized with different NH4 +/NO3 ? proportion at either 1 or 10 mM N. In both species, N forms had small effects at low N concentration, but at high N concentration they markedly affected the plant performance. A greater proportion of NH4 + in the fertilizer at high N caused toxicity as it reduced growth and caused seedling death, with the effect being greater in Q. ilex than in P. halepensis. An increase in the proportion of NO3 ? at high N strongly enhanced growth relative to low N plants in P. halepensis but had minor effects in Q. ilex. Relatively more NH4 + in the fertilizer enhanced plant P concentration but reduced K concentration in both species, while the opposite effect occurred with NO3 ?, and these effects were enhanced under high N concentration. We conclude that species responses to inorganic N forms were related to their ecology. P. halepensis, a pioneer tree, had improved performance with NO3 ? at high N concentration and showed strong plasticity to changes in N supply. Q. ilex, a late successional tree, had low responsiveness to N form or concentration. 相似文献
8.
Anne Ganteaume Mercedes Guijarro Marielle Jappiot Carmen Hernando Corinne Lampin-Maillet Pedro Pérez-Gorostiaga José A. Vega 《Annals of Forest Science》2011,68(3):531-541
• Introduction
Wildfires are considered the most important disturbance in the Mediterranean Basin, and some are propagated over long distances due to lift-off and ignition of firebrands. 相似文献9.
In most eukaryotes cytokinesis is brought about by a contractile actin ring located at the division plane. Here, in fission yeast the actin ring was found to be required to generate late-mitotic microtubular structures located at the division plane, and these in turn maintained the medial position of the actin ring. When these microtubular structures were disrupted, the actin ring migrated away from the cell middle in a membrane traffic-dependent manner, resulting in asymmetrical cell divisions that led to genomic instability. We propose that these microtubular structures contribute to a checkpoint control that retains the equatorial position of the ring when progression through cytokinesis is delayed. 相似文献
10.
Thornton TM Pedraza-Alva G Deng B Wood CD Aronshtam A Clements JL Sabio G Davis RJ Matthews DE Doble B Rincon M 《Science (New York, N.Y.)》2008,320(5876):667-670
Glycogen synthase kinase 3beta (GSK3beta) is involved in metabolism, neurodegeneration, and cancer. Inhibition of GSK3beta activity is the primary mechanism that regulates this widely expressed active kinase. Although the protein kinase Akt inhibits GSK3beta by phosphorylation at the N terminus, preventing Akt-mediated phosphorylation does not affect the cell-survival pathway activated through the GSK3beta substrate beta-catenin. Here, we show that p38 mitogen-activated protein kinase (MAPK) also inactivates GSK3beta by direct phosphorylation at its C terminus, and this inactivation can lead to an accumulation of beta-catenin. p38 MAPK-mediated phosphorylation of GSK3beta occurs primarily in the brain and thymocytes. Activation of beta-catenin-mediated signaling through GSK3beta inhibition provides a potential mechanism for p38 MAPK-mediated survival in specific tissues. 相似文献