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1.
2.
Satoshi KAMBAYASHI Masaya IGASE Kosuke KOBAYASHI Ayana KIMURA Takako SHIMOKAWA MIYAMA Kenji BABA Shunsuke NOGUCHI Takuya MIZUNO Masaru OKUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1405-1412
Hypoxic conditions in various cancers are believed to relate with their
malignancy, and hypoxia inducible factor-1α (HIF-1α) has been shown to be a major
regulator of the response to low oxygen. In this study, we examined HIF-1α expression in
canine lymphoma using cell lines and clinical samples and found that these cells expressed
HIF-1α. Moreover, the HIF-1α inhibitors, echinomycin, YC-1 and 2-methoxyestradiol,
suppressed the proliferation of canine lymphoma cell lines. In a xenograft model using
NOD/scid mice, echinomycin treatment resulted in a dose-dependent regression of the tumor.
Our results suggest that HIF-1α contributes to the proliferation and/or survival of canine
lymphoma cells. Therefore, HIF-1α inhibitors may be potential agents to treat canine
lymphoma. 相似文献
3.
Nighttime respiration was measured at monthly intervals over one year on the aboveground parts of five sample trees in an 8-year-old hinoki cypress (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.) stand, by an enclosed standing-tree method. The respiration rate rose rapidly from early spring to a maximum in June, and decreased abruptly in July and then gradually toward autumn and winter. The seasonal change in the respiration rate was synchronized with stem volume increment rather than with monthly mean air temperature. The respiration rate, r, of individual trees increased with increasing tree dimensions, such as stem volume, v(S), and stem girth at the base of the live crown, G(B). The dependence of respiration rate on tree size was successfully represented by a power function. The r - v(S) dependence was rather stronger than the r - G(B) (2) dependence, especially toward the end of the growing season (from July to September). The observed respiration rate was almost the same as the respiration rate corrected for the monthly mean air temperature. The annual respiration of individual trees was directly proportional to their phytomass or to its increment. Although the annual respiration of individual trees decreased proportionally to the square root of the leaf mass, it decreased abruptly in the range close to the smallest sample tree. Combining the monthly relationship between respiration rate and stem volume with the tree size distribution in the stand, the stand aboveground annual respiration was estimated to be 20.4 Mg CO(2) ha(-1) year(-1) (= 12.5 Mg dry mass ha(-1) year(-1)) for an aboveground biomass of 17.4 Mg ha(-1) with an annual increment of 6.51 Mg ha(-1) year(-1), i.e., the stand aboveground annual respiration amounted to the equivalent of 72% of the biomass or to almost twice the biomass increment. 相似文献
4.
T. Ona T. Sonoda K. Ito M. Shibata Y. Tamai Y. Kojima J. Ohshima S. Yokota N. Yoshizawa 《Wood Science and Technology》2001,35(3):229-243
Relationships between cell and pulp properties were investigated by examining the within-tree property variations in Eucalyptus camaldulensis and Eucalyptus globulus. Properties investigated included proportions of ray and axial parenchyma, thickness of cell walls and cell wall percentages.
The characteristics of the ray and axial parenchyma (their proportions and wall thickness) were found to have a significant
influence on all measured pulp properties, including paper strength properties. Multiple regression of pulp properties in
relation to cell properties revealed that nearly all measured pulp properties were explained by cell properties at the 1%
significance level. It was concluded, therefore, that all cell types are important for predicting pulp properties, and it
is strongly recommended that tree breeding programs for Eucalyptus include the measurement of all cell types.
Received 6 July 1999 相似文献
5.
F.?Ishiguri S.?Maruyama K.?Takahashi Z.?Abe S.?Yokota M.?Andoh N.?YoshizawaEmail author 《Journal of Wood Science》2003,49(2):135-139
Sugi green logs with red or black heartwood were smoke-heated, and the changes in the color of the heartwood after ultraviolet
(UV) (λ = 365 nm) radiation exposure were then observed. After UV radiation exposure, the redness and yellowness increased in both
the red and black heartwoods, whereas the brightness decreased. In the black heartwood, the resulting color turned from yellowish
white to reddish brown. Reddening in black heartwood after exposure to a combination of smoke heating and UV radiation is
thought to be due to a decrease in brightness and an increase in both redness and yellowness. However, the degree of change
in heartwood color by UV radiation exposure was not greatly affected by smoke-heating treatments of various lengths. When
methanol extracts were fractionated and exposed to UV radiation, the yellowness increased in the n-hexane-soluble portion and the redness increased in the acetone-soluble fractions from the n-hexane-insoluble portion. These results suggest that the n-hexane-soluble fraction contains the substances that allow heartwood color to change to yellow after UV radiation exposure,
and the acetone-soluble-fraction from the n-hexane-insoluble portion contains the substances that allow it to change to red.
Received: November 14, 2001 / Accepted: June 3, 2002
Acknowledgment This research was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of
Science.
This study was presented in part at the 51st Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001
Correspondence to:N. Yoshizawa 相似文献
6.
Stephen Adu-Bredu Taketo Yokota Kazuharu Ogawa Akio Hagihara 《Journal of Forest Research》1997,2(1):31-37
The study was carried out in a 9-year-old hinoki cypress (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.), stand over a span of three years from July 1992 to June 1995, primarily to predict litter production
from exteral tree dimensions by combining open-top clothtrap and clipping methods. Litter production was virtually concentrated
in October and November. Stem cross-sectional area at the crown base was proved to be the reliable predictor of litter production,
and that single regression model was evolved irrespective of year. The regression model had proportional constants of 2.696
× 10−2 and 3.540 × 10−2 kg cm−2 year−1 for leaf litter and total litter production, respectively. Utilizing the model, leaf litter production of the stand was assessed
to be 5.04, 5.12, and 4.99, and total litter production to be 6.48, 6.58, and 6.40 Mg ha−1 year−1 for the first, second and third year, respectively. Biomass increment was 6.67, 7.80, and 7.70, tree mortality was 0.15,
0.13, and 0.41, and insect grazing was 0.09, 0.05, and 0.002 Mg ha−1 year−1 for the first, second and third year, respectively. Above-groud net production was therefore 13.39, 14.55, and 14.51, Mg
ha−1 year−1, and biomass accumulation ratio (biomass/net production) was 1.86, 2.21, and 2.76 year for the first, second and third year,
respectively. Considering data from earlier studies and the results of this study, biomass accumulation ratio,BAR (year), of hinoki stands was best related to above-ground biomass,y (Mg ha−1), using the power function:BAR=0.112y
0.936. Excluding seedling stands, leaf efficiency (above-ground net production per unit leaf mass) of hinoki stands was 0.91±0.02
(SE) Mg Mg−1 year−1, irrespective of stand biomass or age. 相似文献
7.
Tomonari Kotani Masashi Yokota Hiroshi Fushimi Seiichi Watanabe 《Fisheries Science》2011,77(2):255-261
Survival in larval rearing experiments is difficult to estimate due to accidental losses and periodic sampling. The number
of sampled fish can be a large proportion of the stocked ones, making it difficult to calculate the overall survival rate
and mortality coefficient as this is based on the initial number. Here, a new method of calculating survival is proposed using
the mortality coefficient. When the initial stocking density and sampled and final numbers are known, and assuming that mortality
coefficient is constant, the final number of fishes can be represented by the formula N
t = e−mt
(N
0 − ΣN
Sne
mdn), where t is rearing period (days), N
0 indicates initial number, N
t indicates the survival number at t days of rearing, m is the natural mortality coefficient, N
Sn is the sampled number in the nth sampling, and dn is the rearing period until removal of the nth sample. The provisional mortality coefficient is calculated from initial and final stocking numbers. Then values for the
natural mortality coefficient are substituted into the formula with successive approximation. The coefficient, which most
closely approximates the actual survival, is determined as the best fit natural mortality coefficient. Examples of larval
experiments are provided to demonstrate the method and show that survival is often underestimated using traditional methods. 相似文献
8.
Sato D Awad AA Chae SH Yokota T Sugimoto Y Takeuchi Y Yoneyama K 《Journal of agricultural and food chemistry》2003,51(5):1162-1168
A simple and rapid analytical method for strigolactones, germination stimulants for the root parasitic weeds witchweed (Striga spp.) and broomrape (Orobanche spp.), has been developed using high-performance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). The natural strigolactones (strigol, sorgolactone, orobanchol, and alectrol) were clearly separated and identified by LC/MS/MS. As low as 0.1 pg/microL of strigol and 0.5 pg/microL of sorgolactone could be quantified, whereas 1 pg/microL was needed for the quantification of orobanchol (S/N > 10). Using this method, it was found that red clover produces orobanchol and alectrol but not strigol. The roots of red clover seedlings were found to produce 13, 70, 58, and 65 pg of orobanchol/plant 1, 2, 3, and 4 weeks after germination, respectively. 相似文献
9.
Mullen W McGinn J Lean ME MacLean MR Gardner P Duthie GG Yokota T Crozier A 《Journal of agricultural and food chemistry》2002,50(18):5191-5196
Analysis of extracts of Glen Ample raspberries (Rubus idaeus L.) by gradient, reverse phase HPLC with diode array and tandem mass spectrometry identified eleven anthocyanins, including cyanidin-3-sophoroside, cyanidin-3-(2(G)-glucosylrutinoside), cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-sophoroside, pelargonidin-3-(2(G)-glucosylrutinoside), and pelargonidin-3-glucoside. Significant quantities of an ellagitannin, sanguiin H-6, with an M(r) of 1870 were detected along with lower levels of a second ellagitannin, lambertianin C, which has an M(r) of 2804. Other phenolic compounds that were detected included trace levels of ellagic acid and its sugar conjugates along with one kaempferol- and four quercetin-based flavonol conjugates. Fractionation by preparative HPLC revealed that sanguiin H-6 was a major contributor to the antioxidant capacity of raspberries together with vitamin C and the anthocyanins. Vasodilation activity was restricted to fractions containing lambertianin C and sanguiin H-6. 相似文献
10.
At 114 ranches in the Hidaka District of Hokkaido, a total of 1,118 Thoroughbred foals
that were born from January to June 2003 were examined at different times from May to
September of the same year to ascertain the occurrence of clubfoot. Clubfoot was seen in a
total of 179 foals (16%) at 89 ranches (78%). Also, 124 of the 179 foals (69.3%) had
clubfoot of grade II or higher, and it was found that grade I was likely to be overlooked
or rapidly advance. In the present study, the occurrence of clubfoot was higher than
expected in the investigated region. Hence, it is necessary to establish accurate
diagnostic techniques and clubfoot guidelines to minimize the impact. 相似文献