全文获取类型
收费全文 | 1489篇 |
免费 | 130篇 |
专业分类
林业 | 128篇 |
农学 | 43篇 |
基础科学 | 5篇 |
286篇 | |
综合类 | 104篇 |
农作物 | 66篇 |
水产渔业 | 127篇 |
畜牧兽医 | 704篇 |
园艺 | 39篇 |
植物保护 | 117篇 |
出版年
2023年 | 16篇 |
2022年 | 17篇 |
2021年 | 63篇 |
2020年 | 68篇 |
2019年 | 60篇 |
2018年 | 70篇 |
2017年 | 60篇 |
2016年 | 65篇 |
2015年 | 53篇 |
2014年 | 74篇 |
2013年 | 72篇 |
2012年 | 121篇 |
2011年 | 146篇 |
2010年 | 80篇 |
2009年 | 76篇 |
2008年 | 96篇 |
2007年 | 78篇 |
2006年 | 75篇 |
2005年 | 72篇 |
2004年 | 62篇 |
2003年 | 59篇 |
2002年 | 43篇 |
2001年 | 15篇 |
2000年 | 3篇 |
1999年 | 8篇 |
1998年 | 3篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1969年 | 1篇 |
1966年 | 1篇 |
1962年 | 1篇 |
1961年 | 1篇 |
1959年 | 1篇 |
1958年 | 3篇 |
1955年 | 1篇 |
1940年 | 1篇 |
排序方式: 共有1619条查询结果,搜索用时 15 毫秒
1.
Propelled by urbanization, rising incomes, and changing diets, food markets have been expanding in Africa and South Asia, creating the vast potential for job and income opportunities along food supply chains and, hence, for poverty reduction. The novel coronavirus(COVID-19) that spread to a pandemic in early 2020 provokes enormous setbacks to this expansion. This, however, should provide lessons regarding the importance of resilient and inclusive food systems. Emergency responses to COVID-19 should consider interventions towards that end and leverage the opportunities provided by food markets growth as economies recover from the present economic recession. This paper assesses options of how this could be done by facilitating the better functioning and interconnectedness of the many small and medium-sized enterprises that are proliferating along the "hidden middle" of food value chains in storage, logistics, transportation, and wholesale and retail distribution. It also explores how policies can help smallholder farmers connect to this "hidden middle" in more gainful ways and help them climb out of poverty as well. 相似文献
2.
Concetta Maria Messina Simona Manuguerra Rosaria Arena Giuseppe Renda Giovanna Ficano Mariano Randazzo Stefano Fricano Saloua Sadok Andrea Santulli 《Marine drugs》2021,19(4)
Non-edible parts of crustaceans could be a rich source of valuable bioactive compounds such as the carotenoid astaxanthin and peptides, which have well-recognized beneficial effects. These compounds are widely used in nutraceuticals and pharmaceuticals, and their market is rapidly growing, suggesting the need to find alternative sources. The aim of this work was to set up a pilot-scale protocol for the reutilization of by-products of processed shrimp, in order to address the utilization of this valuable biomass for nutraceutical and pharmaceuticals application, through the extraction of astaxanthin-enriched oil and antioxidant-rich protein hydrolysates. Astaxanthin (AST) was obtained using “green extraction methods,” such as using fish oil and different fatty acid ethyl esters as solvents and through supercritical fluid extraction (SFE), whereas bioactive peptides were obtained by protease hydrolysis. Both astaxanthin and bioactive peptides exhibited bioactive properties in vitro in cellular model systems, such as antioxidant and angiotensin I converting enzyme (ACE) inhibitory activities (IA). The results show higher astaxanthin yields in ethyl esters fatty acids (TFA) extraction and significant enrichment by short-path distillation (SPD) up to 114.80 ± 1.23 µg/mL. Peptide fractions of <3 kDa and 3–5 kDa exhibited greater antioxidant activity while the fraction 5–10 kDa exhibited a better ACE-IA. Lower-molecular-weight bioactive peptides and astaxanthin extracted using supercritical fluids showed protective effects against oxidative damage in 142BR and in 3T3 cell lines. These results suggest that “green” extraction methods allow us to obtain high-quality bioactive compounds from large volumes of shrimp waste for nutraceutical and pharmaceutical applications. 相似文献
3.
Rossano Bolpagni Sara Magrini Andrea Coppi Angelo Troìa Janne Alahuhta Marit Mjelde Mattia M. Azzella 《水产资源保护:海洋与淡水生态系统》2021,31(10):2690-2696
- Isoëtes sabatina is the rarest aquatic quillwort in Europe. Although recently found (2013) in Lake Bracciano (central Italy), the species is just one step away from extinction with an estimated population not exceeding 400 individuals and a spatial range of a few hundred square metres.
- Lake Bracciano is a deep, oligo-mesotrophic Mediterranean volcanic lake that has been subjected to human activities. From January to October 2017, the lake experienced a dramatic water level decrease (up to −1.50 m), which significantly affected the littoral zone and the habitat of I. sabatina.
- To improve the chances of survival of I. sabatina, the first eco-taxonomic investigation on this species was carried out to describe its genetic distinctness, physical and chemical requirements and companion species.
- The phylogenetic position of I. sabatina was investigated by applying standard DNA barcoding methods. Simultaneously, during summer 2019, the physical and chemical features of water and sediments of the I. sabatina population and five small Alpine lakes colonized by Isoëtes echinospora – a supposed close relative – were characterized. These data were then compared with the available data on the trophic requirements of the target obligate aquatic Isoëtes, together with Isoëtes lacustris and Isoëtes malinverniana.
- The present survey confirmed the taxonomic and ecological distinctness of I. sabatina – providing the first evidence of genetic differentiation from I. echinospora. Isoëtes sabatina grows in waters with temperature, conductivity and total alkalinity up to 30°C, 561 μS cm−1 and 3.45 meq L−1, respectively.
- The edaphic requirements of I. sabatina confirm its outstanding conservation value, and this study offers a basic understanding of how to prevent its extinction. Now, all possible actions must be taken immediately to save this species.
4.
Detection and characterization of a rhabdovirus causing mortality in black bullhead catfish,Ameiurus melas 下载免费PDF全文
Giulia Bedendo Valentina Panzarin Andrea Fortin Gianpiero Zamperin Tobia Pretto Alessandra Buratin Rosita Quartesan Matteo Sabbion Cristian Salogni Francesco Pascoli Anna Toffan 《Journal of fish diseases》2018,41(7):1063-1075
This study fully describes a severe disease outbreak occurred in 2016 in black bullhead catfish farmed in Italy. Affected fish showed nervous clinical signs as well as emaciations and haemorrhagic petechiae on the skin at the fin bases, abdomen and gills. Viral isolation in cell culture allowed the subsequent identification of a rhabdovirus, tentatively named ictalurid rhabdovirus (IcRV), through electron microscopy, immunofluorescence and whole genome sequencing (WGS). The newly isolated virus, together with 14 additional viral strains stored in our repository and detected during similar mortality episodes in the period 1993–2016, was phylogenetically analysed on the basis of the nucleoprotein and the glycoprotein nucleotide and amino acid sequences. The genetic distances among Italian IcRV strains were also estimated. Our results show that all the IcRV strains belong to the genus Sprivivirus and are closely related to the tench rhabdovirus (TenRV). Italian catfish production is constantly decreasing, mainly due to viral infections, which include the newly characterized IcRV. Data presented in this work will assist to investigate the molecular epidemiology and the diffusive dynamics of this virus and to develop adequate surveillance activities. 相似文献
5.
Pedro A. Castrillo Catuxa Varela-Dopico Paz Ondina María Isabel Quiroga Roberto Bermúdez 《Journal of fish diseases》2020,43(1):69-80
Freshwater mussels of the order Unionida encyst into the fish mucosa to metamorphose and complete their life cycle, causing a parasitic disease known as glochidiosis. This parasitic stage represents a bottleneck for the survival of naiads, particularly for critically endangered species as Margaritifera margaritifera; however, little is known about the events occurring during this critical stage. Therefore, this study aimed to histologically characterize the development of M. margaritifera glochidiosis in Atlantic salmon to get insight into the pathogenesis of this interaction. Fish exposed to glochidia were sampled during the first 44 days post-exposure, and organs were observed by stereomicroscopy and light microscopy. Glochidia attached to the gills by pinching the lamellar epithelium, whereupon an acute proliferative branchitis engulfed most of the larvae. However, during the first 14 days, a severe detachment of unviable glochidia occurred, associated with the presence of pleomorphic inflammatory infiltrate and epithelial degeneration. In the cases where larvae remained attached, a chronification of the lesions with none to scarce inflammation was observed. These results provide key information to better understand the complex host–parasite interaction during the early stages of glochidiosis and provide valuable information to optimize artificial rearing of naiads in conservation of threatened freshwater mussel populations. 相似文献
6.
Matthew L. Peterson Dana J. Lee John Montgomery Michael Hellmair Andrea Fuller Doug Demko 《Fisheries Management and Ecology》2020,27(4):399-416
The spatial and temporal distribution of spawning activity by autumn‐run Chinook salmon Oncorhynchus tshawytscha (Walbaum) was examined across multiple years. The study period included two years of extreme drought conditions when water temperatures in the spawning reach of the Stanislaus River were considered sub‐optimal for spawning and egg incubation. Despite varying levels of superimposition, redd counts and densities remained stable and positively associated with river location, indicating that superimposition may be driven by habitat preference rather than the absence of suitable spawning locations. Spawning occurred slightly later during drought years (6–10 days later compared with wetter years). This delay was attributable to deferred migration instead of deferred spawning, and the majority of redds were constructed at water temperatures exceeding the optimal temperature range. As a consequence, estimates of juvenile production during 2014 and 2015 were among the lowest on record. These findings may be related in part to the high hatchery contribution to the population, above‐average temperatures during spawning and incubation, and superimposition rates. Management recommendations include adequate cold‐water storage in the upstream reservoir, refined spawning habitat restoration techniques in the light of superimposition rates and, on a broader system scale, actions that reduce the amount of stray hatchery Chinook salmon. 相似文献
7.
Carla Bacchetta Andrea S. Rossi Raúl E. Cian Silvina R. Drago Jimena Cazenave 《Aquaculture Nutrition》2019,25(4):761-769
This study was conducted to investigate the effects of dietary synthetic β‐carotene on growth performance, haematological and immunological parameters, energy reserves and antioxidant status of juvenile fish Piaractus mesopotamicus. Two hundred and ten fish (12.24 ± 2.98 g) were fed with a control extruded feed (CEF) and the same diet supplemented with β‐carotene (BEF) for 90 days. A higher weight gain and specific growth rate were found in fish fed BEF in comparison with those fed CEF. In addition, BEF‐fed fish showed lower liver somatic index and a higher percentage of eosinophils. No statistical differences were found in energy reserves, except for plasma triglycerides which decreased in fish fed with BEF. Regarding oxidative stress markers, decreased activities of antioxidant enzymes were found in intestine and gills of BEF‐fed fish (SOD and GST, respectively). In the liver, both LPO levels and CAT activity decreased in fish fed with BEF. Additionally, lower brain LPO levels without changes in the antioxidant enzymes were observed in BEF‐fed fish. The inclusion of dietary synthetic β‐carotene improved growth and antioxidant status, and had a plasma triglyceride‐lowering effect in juvenile P. mesopotamicus. 相似文献
8.
Attilio L. Mordenti Nico Brogna Giorgia Canestrari Elena Bonfante Stefano Eusebi Ludovica M. E. Mammi Elisa Giaretta Andrea Formigoni 《Animal Science Journal》2019,90(5):619-627
Focus of this study was to evaluate the most suitable breed/crossbred and the appropriate nutritional strategies to increase marbling in beef muscle and to improve its healthy properties, in particular the n‐3 fatty acids content. One hundred and seventy‐six heifers of three crossbreed commonly reared by Emilia‐Romagna farmers: 48 Bleu Belge × Freisian (ITA), 48 Charolais × Aubrac (FRA), and 80 Angus (ANG) were used. Animals of each breed were randomly subdivided in two experimental groups that received two diets, differing for the dietary lipid source. Control group (C) received a basal diet containing protected vegetable fats, whereas treated one (T) received the same basal diet supplemented with 0.9 kg/head/day of extruded flaxseed. After slaughtering, a sample of Longissimus thoracis was collected from each animal for meat quality analysis. Our results demonstrated that, in a shorter fattening period, ANG animals obtained the best dry matter intake, average daily gain and the best fattening scores. ITA and ANG meat presented the highest marbling scores. ANG breed had the highest amount of C18:1, the highest unsaturated/saturated fatty acids ratio and the lowest n‐6/n‐3 ratio. The T animals, independently from breed, showed the highest amount of α‐linolenic acid (ALA). In addition, ANG‐T meat presented the highest ALA content. 相似文献
9.
Monica Puccini Lorenzo Guazzelli Andrea Luca Tasca Andrea Mezzetta Christian Silvio Pomelli 《Water, air, and soil pollution》2018,229(7):239
Thallium is an emerging contaminant, which can be retained in scale encrustation within the pipeline and then released to drinking water. It is included in the Priority Pollutant List of the U.S. EPA. In this study, a sample from the water pipeline of Pietrasanta (Italy), affected by the contamination of thallium, is characterized by SEM-EDS, TGA, and FT-IR. Fluorescence spectroscopy is then proposed as the optimal technique for the detection of the contaminant. The functionality of a previously reported fluorescent calix[4]arene-based chemosensor is verified first on a standard solution of thallium nitrate and then on the sample under investigation. The quenching of the fluorescence of the sensor during the complexation of thallium is confirmed, identifying an ON-OFF sensor with high sensitivity, able to detect concentrations as low as 10?6 M and with high potential of development for the in situ and fast monitoring of the pollutant in the water network. 相似文献
10.
Jakub Vozaf Alexander V. Makarevich Andrej Balazi Jaromir Vasicek Andrea Svoradova Lucia Olexikova Peter Chrenek 《Animal Science Journal》2021,92(1):e13670
The aim of our study was to examine effects of the length of semen equilibration as well as two freezing techniques on ram sperm post-thaw quality. The ejaculates of Wallachian sheep rams (n = 12) were collected by an electro-ejaculation, equilibrated in a Triladyl® (0, 2, 4, 6, and 8 h) containing glycerol and egg yolk and frozen by programmable freezing (PF) or manual freezing (MF). After thawing, sperm samples were subjected to the motility (computer-assisted sperm analysis [CASA]), viability (SYBR-14/PI), and fertilizing ability (FA) (in vitro penetration/fertilization test on bovine oocytes) assays. It was found that the equilibration of 6 h (E-6) ensured higher post-thaw sperm motility and progressive movement compared with other lengths tested, irrespective of a freezing technique. The E-6 sperm viability did not differ between PF and MF but was lower (P < 0.05) than control. Sperm FA (E-6) was similar in PF (60.44%) and MF (62%) but slightly lower than in fresh (72.8%). Our data demonstrate that the use of MF was comparable with PF, which can be applied in the field conditions without need in a piece of cost-expensive equipment, which can greatly benefit the gene bank of animal genetic resources. 相似文献