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1.
A novel concept for performing stoichiometric and catalytic chemical transformations has been developed that is based on the limited miscibility of partially or fully fluorinated compounds with nonfluorinated compounds. A fluorous biphase system (FBS) consists of a fluorous phase containing a dissolved reagent or catalyst and another phase, which could be any common organic or nonorganic solvent with limited or no solubility in the fluorous phase. The fluorous phase is defined as the fluorocarbon (mostly perfluorinated alkanes, ethers, and tertiary amines)-rich phase of a biphase system. An FBS compatible reagent or catalyst contains enough fluorous moieties that it will be soluble only or preferentially in the fluorous phase. The most effective fluorous moieties are linear or branched perfluoroalkyl chains with high carbon number; they may also contain heteroatoms. The chemical transformation may occur either in the fluorous phase or at the interface of the two phases. The application of FBS has been demonstrated for the extraction of rhodium from toluene and for the hydroformylation of olefins. The ability to separate a catalyst or a reagent from the products completely at mild conditions could lead to industrial application of homogeneous catalysts or reagents and to the development of more environmentally benign processes. 相似文献
2.
A questionnaire study was performed involving 76 farms with a total of 380,207 pigs to assess the welfare of pigs kept in Hungary based on the animal welfare legislation of the European Union. Most significant deficiencies were found in the fulfillment of the provisions relating to space requirements, the stall microclimate and the behavioural needs of the animals. 相似文献
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The effects of cAMP-elevating compounds IBMX (3-isobutyl-1-methylxanthine) and isoproterenol, and that of rutin (an effective superoxide scavenger) were studied on orthovanadate--(a putative protein-phosphotyrosine phosphatase inhibitor) induced nitric oxide (NO) production in J774A.1 mouse macrophage cells. As we previously reported (Koncz and Horváth, 2000), rutin and sodium orthovanadate act synergistically to induce production of high amount of NO in J774A.1 cells. IBMX, an agent that can elevate cAMP level in the cells, can reduce the production of both the LPS- and rutin + orthovanadate-induced NO in macrophages. In contrast, isoproterenol, a non-selective beta-adrenergic receptor agonist, that reduced the LPS-induced NO production in macrophage cells, was unable to reduce the rutin + orthovanadate-induced NO production without negatively affecting cell viability. Moreover, isoproterenol dramatically enhanced the orthovanadate-induced NO synthesis in J774A.1 cells. Our previous study clarified that rutin and orthovanadate, in a specific concentration ratio of both, were able to produce hydrogen peroxide (H2O2). Using 2',7'-dichlorofluorescein-diacetate as a marker for H2O2, isoproterenol alone induced its oxidation but the rutin plus orthovanadate-induced H2O2 production was reduced by isoproterenol. These observations have revealed that, in some cases, H2O2 and superoxide (O2-) scavengers can act in a reverse mode on macrophage cells depending on the presence or absence of orthovanadate. 相似文献
5.
B. Piros J. Glogowski R. Kolman A. Rzemieniecki J. Domagala Á. Horváth B. Urbanyi A. Ciereszko 《Fish physiology and biochemistry》2002,26(3):289-295
This paper provides data concerning biochemical composition of milt of two sturgeon species, Siberian sturgeon bred in aquaculture
facility of Inland Fisheries Institute in North Poland and sterlet (from two different populations from Danube and Odra).
Milt plasma of Siberian sturgeon and sterlet, when compared to teleost fish, is characterized by much lower osmolality (up
70 to 96 mOsm kg−1) and protein concentration (0.24–0.58 g l−1). In spite of the presence of an acrosome and acrosin the antiproteinase activity of seminal plasma was low (12.79–25.40 U l−1). Activities of arylsulfatase and β-N-acetylglucosaminidase were found in spermatozoa. This agrees with the presence of an
acrosome in sturgeons sperm. Similarly to mammals, these enzymes are also present in milt plasma. We determined a range of
enzymatic activities from the minimal (seminal plasma) to the maximal damage (supernatants obtained after freezing-thawing
without cryoprotectant). Activities of arylsulfatase, β-N-acetylglucosaminidase, lactic dehydrogenase and acid phosphatase
were released from spermatozoa after freezing-thawing. For this reason they are good potential candidates as a markers of
cryoinjury to sperm acrosome and midpiece.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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András Cseri Mátyás Cserháti Maria von Korff Bettina Nagy Gábor V. Horváth András Palágyi János Pauk Dénes Dudits Ottó Törjék 《Euphytica》2011,181(3):341-356
In the present study, allele mining was conducted on a panel of drought related candidate genes in a set of 96 barley genotypes
using EcoTILLING, which is a variant of the targeting induced local lesions in genomes (TILLING) technology. Analyzing approximately
1.5 million basepairs in barley a total number of 94 verified unique haplotypes were identified in 18 amplicons designed for
9 genes. Overall, 185 single nucleotide polymorphisms (SNPs) and 46 insertions/deletions (INDELs) were detected with a mean
of 1SNP/92 bp and 1INDEL/372 bp genomic sequence. Based on overlapping haplotype sequences, markers were developed for four
candidate genes (HvARH1, HvSRG6, HvDRF1, HVA1), which allows distinguishing between the main haplotypes showing either differences in amino acid sequence or which have
larger INDELs in the promoter region. As “proof of concept”, the HvARH1 and HvSRG6 haplotypes were tested for the level of abscisic acid-induced gene expression in subsets of genotypes belonging to different
haplotype categories. An integrated database was developed to contain information about the genes, genotypes, and haplotypes
analyzed in this study. The database supplies profound information about the natural variation in the tested drought related
candidate genes providing a significant asset for further mapping studies dealing with this highly polygenic trait. 相似文献
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L. Horváth 《Aquaculture (Amsterdam, Netherlands)》1977,10(2):161-167
Using the old method of spawning it is impossible to produce wels larvae in quantities sufficient for a special fry farm economically. Because of this, a large-scale intensive method had to be developed, similar to those for other species which are propagated in hatcheries. A method for storing breeders was elaborated which included sewing their mouths shut and consequently eliminating injuries. Pituitary treatment — practised by others before — was applied, and disadvantages of the method (such as the development of furuncles) eliminated. A method for taking sperm from males with the help of a special syphon was developed.To avoid losses, larvae were reared in basins until they reached the size of 2–2.5 cm. Using this method considerable quantities of fry were produced in our hatchery. 相似文献
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