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聂鸿涛  李琪  孔令锋 《水产学报》2013,37(2):207-215
为提高微卫星分型效率,从以往报道的皱纹盘鲍微卫星中筛选出易扩增、特异性好的微卫星位点进行组合扩增,并通过优化退火温度、反应体系、引物浓度等条件,开发了4组多重PCR扩增体系.运用CERVUS3.0软件对12个皱纹盘鲍全同胞家系的372个子代进行家系鉴定,验证了这4组多重PCR在家系鉴定中的效率.结果发现,仅用1组微卫星多重PCR模拟和实际家系鉴定的成功率分别为86%和90%,两组则达到100%.结果表明,微卫星多重PCR技术能准确地把任意子代鉴定至其所属家系,可以进行大批量家系材料分析,具有较好的应用价值.  相似文献
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In order to explore the distribution of different taste buds in the barbells of yellow catfish (Pelteobagrus fulvidraco) collected from the Yangtze River, the quantity, morphology and distribution of different taste buds in the barbells was studied by bright-field optical microscope and scanning electron microscopy. The taste buds in the barbell were predominantly localized at the middle two thirds regions of the barbells, and could be categorized into three major types based on their morphological and histological features. Type I and II TB were distributed on the elevated layer of the surrounding epithelium, while Type III TB ended apically at the level with the epithelium. Significant quantitative differences (p < 0.05) in the TB number within unit length barbell were observed between NB and MB (p = 0.00001), NB and MIB (p = 0.00758), and NB and MOB (p = 0.00209); no significant differences (p > 0.05) were found between MB and MIB (p = 0.05293), MB and MOB (p = 0.05994) and MIB and MOB (p = 0.08320). The number, distribution and morphological variability of TBs could be a consequence of adaptation to the environment. α-Gustducin immunofluoresence signals were detected in cells of all types of TBs. The strong expression of α-gustducin on the barbells of catfish suggests that the taste-induced signal transduction in taste cells was common to all vertebrates.  相似文献
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