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Yuto SANO Kazuya MATSUDA Minoru OKAMOTO Kazushige TAKEHANA Kazuko HIRAYAMA Hiroyuki TANIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):287-291
Antigen-presenting cells (APCs) in the uveal tract participate in ocular immunity including immune
homeostasis and the pathogenesis of uveitis. In horses, although uveitis is the most common ocular disorder,
little is known about ocular immunity, such as the distribution of APCs. In this study, we investigated the
distribution of CD163-positive and MHC II-positive cells in the normal equine uveal tract using an
immunofluorescence technique. Eleven eyes from 10 Thoroughbred horses aged 1 to 24 years old were used.
Indirect immunofluorescence was performed using the primary antibodies CD163, MHC class II (MHC II) and CD20.
To demonstrate the site of their greatest distribution, positive cells were manually counted in 3 different
parts of the uveal tract (ciliary body, iris and choroid), and their average number was assessed by
statistical analysis. The distribution of pleomorphic CD163- and MHC II-expressed cells was detected
throughout the equine uveal tract, but no CD20-expressed cells were detected. The statistical analysis
demonstrated the distribution of CD163- and MHC II-positive cells focusing on the ciliary body. These results
demonstrated that the ciliary body is the largest site of their distribution in the normal equine uveal tract,
and the ciliary body is considered to play important roles in uveal and/or ocular immune homeostasis. The data
provided in this study will help further understanding of equine ocular immunity in the normal state and might
be beneficial for understanding of mechanisms of ocular disorders, such as equine uveitis. 相似文献
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Sho FUKUI Yoshifumi ENDO Kazuko HIRAYAMA Hiroyuki TANIYAMA Tsuyoshi KADOSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):747-751
Simultaneous removal of bilateral thyroid tumors was performed while preserving the
parathyroid gland in six dogs. At least one external parathyroid gland was identified in
all dogs. In five cases, the external parathyroid gland and its blood supply were
preserved intact. In one dog, the vessels supplying the external parathyroid gland had
been invaded by the tumor, and the gland was thus removed and reimplanted into the
sternohyoid muscle. That dog required postoperative treatment with oral calcium gluconate
and vitamin D3. Local tumor recurrence was not observed in any of the cases.
The mean survival time was 920 days. We found that the external parathyroid gland could be
identified and preserved in most dogs undergoing total thyroidectomy. 相似文献
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Hiroki HIRAYAMA Akira FUJIKAWA Soichi KAGEYAMA Satoru MORIYASU Ken SAWAI Sadao ONOE Akira MINAMIHASHI 《Animal Science Journal》2008,79(5):554-560
This study examined genetic diagnosis using whole genome amplification (WGA) in bovine embryos. The first experiment was conducted to compare the WGA efficiency of primer extension preamplification-PCR (PEP-PCR) and multiple displacement amplification (MDA), and to optimize the DNA extraction method. The sensitivity of SRY -specific PCR from MDA products increased when DNA of fibroblasts was extracted by a NaOH treatment instead of the conventional method (heat treatment). The detectability of SRY from PEP-PCR products was lower than that in MDA regardless of the DNA extraction method (proteinase K or NaOH treatment). Sexing and genotyping were performed using MDA products from embryo biopsy. The accuracy of PCR-based and LAMP-based sexing was 100% regardless of the amounts of biopsy. Genotyping of CL16 , BND3 , SCD and F11 in MDA products from 10 to 20% of trophectoderm was successful 97, 97, 95 and 95% of the time, respectively, but reduced biopsy amount (<10% of trophectoderm) decreased the accuracies (33–83%). Microsatellite markers were analyzed using MDA products from 10 to 20% of trophectoderm. In eight out of 16 microsatellites, genotypes were not contradictory among the dam, sire and embryos. In the other eight microsatellites, the inconsistency rates were 17–83%. These results indicate that MDA is useful for multiple genetic diagnoses in bovine embryos. 相似文献
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Koji MISUMI Yuri HIRAYAMA Misae SUZUKI Michiko NAKAI Hiroyuki KANEKO Junko NOGUCHI Kazuhiro KIKUCHI 《The Journal of reproduction and development》2014,60(3):246-249
The present study was conducted to examine the feasibility of in vitro embryo production and transfer
technologies for producing Middle White piglets. After collection from three retired Middle White sows, a total of 222
oocytes were matured, fertilized and cultured in vitro, and a total of 50 embryos from the 4-cell to
blastocyst stage were produced by the 4th or 5th day. These embryos were transferred individually into three recipients along
with 5 in vivo-derived Duroc blastocysts. All of the recipients became pregnant, and they farrowed a total
of 9 Middle White and 9 Duroc piglets. These results suggest that in vitro embryo production using ovaries
from retired sows is useful for reproduction of pigs of pure breeds including the Middle White for breeding activities and
conservation/utilization of genetic resources. 相似文献
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Hoang Le HUY Nobuo KOIZUMI Harimurti NURADJI Susanti Susan M. NOOR NLP Indi DHARMAYANTI Takeshi HAGA Kazuhiro HIRAYAMA Kozue MIURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):531
The prevalence of antimicrobial resistance (AMR) in small mammals dwelling in the city was used as an indicator of AMR bacteria in the environment. We captured 87 small mammals (79 brown rats and 8 house shrews) in four markets, Bogor, Indonesia in October 2019, and we obtained 20 AMR Escherichia coli (E. coil) from 18 brown rats and two house shrews. Of these, eight isolates were determined to be multi-drug resistant (MDR) E. coli, suggesting the potential contamination of AMR E. coli in the markets in Bogor, Indonesia, and that mammals, including humans, are at risk of infection with AMR E. coli from environment. 相似文献
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