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Carbonari Caio Antonio Gomes Giovanna Larissa Gimenes Cotrick Krenchinski Fábio Henrique Simões Plinio Saulo Batista de Castro Edicarlos Velini Edivaldo Domingues 《New Forests》2020,51(4):723-737
New Forests - Eucalyptus harvests generate residues that remain on the soil and affect the efficacy of preemergent herbicides in the following planting cycle. The objective of this work was to... 相似文献
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MULTIDETECTOR‐ROW COMPUTED TOMOGRAPHY PATTERNS OF BRONCHOESPHAGEAL ARTERY HYPERTROPHY AND SYSTEMIC‐TO‐PULMONARY FISTULA IN DOGS 下载免费PDF全文
Gianluca Ledda Marco Caldin Giorgia Mezzalira Giovanna Bertolini 《Veterinary radiology & ultrasound》2015,56(4):347-358
Anomalies involving arterial branches in the lungs are one of the causes of hemoptysis in humans and dogs. Congenital and acquired patterns of bronchoesophageal artery hypertrophy have been reported in humans based on CT characteristics. The purpose of this retrospective study was to describe clinical, echocardiographic, and multidetector computed tomography features of bronchoesophageal artery hypertrophy and systemic‐to‐pulmonary arterial communications in a sample of 14 dogs. Two main vascular patterns were identified in dogs that resembled congenital and acquired conditions reported in humans. Pattern 1 appeared as an aberrant origin of the right bronchoesophageal artery, normal origin of the left one, and enlargement of both the bronchial and esophageal branches that formed a dense network terminating in a pulmonary artery through an orifice. Pattern 2 appeared as a normal origin of both right and left bronchoesophageal arteries, with an enlarged and tortuous course along the bronchi to the periphery of the lung, where they communicated with subsegmental pulmonary arteries. Dogs having Pattern 1 also had paraesophageal and esophageal varices, with the latter being confirmed by videoendoscopy examination. Authors conclude that dogs with Pattern 1 should be differentiated from dogs with other congenital vascular systemic‐to‐pulmonary connections. Dogs having Pattern 2 should be evaluated for underlying pleural or pulmonary diseases. Bronchoesophageal artery hypertrophy can be accompanied by esophageal venous engorgement and should be included in the differential diagnosis for esophageal and paraesophageal varices in dogs. 相似文献
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The aim of this research was to validate the results obtained previously by purge and trap (PT) and to investigate the ability of solid phase microextraction (SPME), a more rapid and less expensive technique, to discriminate drinking milk subjected to different heat treatments (i.e., pasteurization, ultrahigh temperature, "in-bottle" sterilization) and produced at different factories. The data obtained by both methods were processed by multivariate statistical analysis. PT and SPME showed comparable repeatability, although with different performances for the yield of extraction, and allowed the three milk categories to be distinguished. Within the chemical class of methyl ketones, 2-heptanone was found to be the most discriminating compound, and the possibility of using the concentration of this volatile as a marker for heat treatment was investigated. 相似文献
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Mark S Lambert Giovanna Massei Jennifer Bell Leslie Berry Carol Haigh David P Cowan 《Pest management science》2009,65(11):1215-1218
BACKGROUND: Rose‐ringed parakeets Psittacula krameri (Scop.) have recently become established in several European countries, with potential for significant negative economic and ecological impacts. However, in northern Europe the potential for reproductive output is largely unknown. In 2005 the authors established a captive outdoor colony in north‐east England and examined breeding success over 2 years. RESULTS: In 2006 (19 pairs, 15 clutches) the average first clutch size was 3.6 (±0.3) eggs. Six clutches were infertile, and overall the colony produced 1.4 (±0.5) fertile eggs per pair. Eleven pairs produced a second clutch following removal of the first; seven were infertile, and overall productivity was 0.7 (±0.4) fertile eggs per pair. Unsuccessful pairs were rearranged or replaced. In 2007, overall productivity was 2.5 (±0.4) and 1.8 (±0.4) fertile eggs per pair for the first and second attempts respectively. For pairs that remained unchanged through 2006–2007, productivity was consistent between years and breeding attempts. CONCLUSION: Where food and nest sites were not limiting, clutch sizes in north‐east England were similar to those in the native range, and consistent between first and second attempts. This has implications for the future expansion and management of the species. © Crown Copyright 2009. Reproduced with permission of Her Majesty's Stationery Office. Published by John Wiley & Sons, Ltd. 相似文献
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Maria Antonietta Demontis Santa Olga Cacciola Marcella Orrù Virgilio Balmas Valentina Chessa Bianca Elena Maserti Laura Mascia Francesco Raudino Gaetano Magnano di San Lio Quirico Migheli 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(4):339-351
Real-time PCR assays based on SYBR? Green I and TaqMan? technologies were developed for in planta detection and quantification of Phoma tracheiphila, the mitosporic fungus causing ‘mal secco’ disease on citrus. Primers and a hybridization probe were designed on the basis
of the internal transcribed spacer (ITS) region of the nuclear rRNA genes. The real-time PCR assays were compared with a classic
isolation method in two separate experiments carried out on 6 and 24 month-old sour orange seedlings, artificially inoculated
with a conidial suspension of the pathogen. Both technologies made it possible to follow the progression of infection by P. tracheiphila, enabling detection and quantification of the target fungus prior to the development of symptoms. The detection limit was
10 copies of the cloned target sequence and 15 pg of genomic DNA extracted from fungal spores. The values of the cycle threshold
(Ct) were linearly correlated with the concentration of the target DNA, indicating that the method is suitable as a qualitative
and quantitative assay. The presence of non-target fungal DNA had no effect on the specificity of the assay, but resulted
in a 10-fold reduction of sensitivity. Total inhibition of the reaction occurred when conidia of the target pathogen were
mixed with an organic soil substrate before extracting DNA using the standard protocol, while an alternative purification
kit resulted in a significant decrease in sensitivity. Compared to classic methods, real-time PCR proved faster and easier
to perform and showed a higher sensitivity. These results suggest that real-time PCR, based on both chemistries, has a great
potential for early diagnosis of ‘mal secco’ disease and for quantitative estimation of fungal growth within host tissue. 相似文献
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