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1.
Immunohistochemical differentiation of reactive from malignant mesothelium as a diagnostic aid in canine pericardial disease 下载免费PDF全文
E. Milne Y. Martinez Pereira C. Muir T. Scase D. J. Shaw G. McGregor L. Oldroyd E. Scurrell M. Martin C. Devine H. Hodgkiss‐Geere 《The Journal of small animal practice》2018,59(5):261-271
Objectives
To develop a provisional immunohistochemistry panel for distinguishing reactive pericardium, atypical mesothelial proliferation and mesothelioma in dogs.Materials and Methods
Archived pericardial biopsies were subject to haematoxylin and eosin staining, immunohistochemistry for cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Samples were scored for intensity and number of cells stained.Results
Ten biopsies of reactive mesothelium, 17 of atypical mesothelial proliferation, 26 of mesothelioma and five of normal pericardium were identified on the basis of haematoxylin and eosin staining. Cytokeratin and vimentin were expressed in all biopsies, confirming mesothelial origin. Normal pericardial samples had the lowest scores for insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Mesothelioma and atypical proliferative samples were similar to each other, with higher scores for insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 than the reactive samples. Desmin staining was variable. Insulin‐like growth factor II mRNA‐binding protein 3 was the best to distinguish between disease groups.Clinical Significance
An immunohistochemistry panel of cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 could provide superior information compared with haematoxylin and eosin staining alone in the diagnosis of cases of mesothelial proliferation in canine pericardium, but further validation is warranted. 相似文献2.
Adams Alison B. Pontius Jennifer Galford Gillian Gudex-Cross David 《Landscape Ecology》2019,34(10):2401-2419
Landscape Ecology - Understanding how the Northern Forest landscape has changed and is likely to change, both in terms of forest extent and forest configuration, has important implications for... 相似文献
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An exercise in leadership training for veterinary students aiming for careers in biomedical research
A group discussion on the theme of "leadership" has been a central event in the annual Cornell Leadership Program for Veterinary Students since 1990. However, these discussions were often unfocused and did not readily demonstrate the leadership skills of distinguished guests who were invited to participate. Since 1998, a new format for this session has been developed in which students and guests are assigned individual roles in a scenario that is unfolded by a moderator over two to three hours. This role-playing exercise ensures that every student is obliged to participate and has an opportunity to practice such leadership skills as critical thinking, verbal communication, and decision making under pressure and with inadequate information. The distinguished guests, in their assigned roles, are able to interact freely with the student fellows and thus demonstrate their expertise as experienced leaders. This challenging experience has become an enjoyable part of the 10-week Leadership Program and one that shows the importance of leadership skills for those who aspire to careers in the biomedical sciences. 相似文献
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Evolution of C2H4 from soils under anaerobic conditions was stimulated by amendment with cereal straw, but hay had little effect. Temporary restoration of aerobic conditions resulted in large increases in C3 and C4 hydrocarbons, with both straw and hay amendments. Several known products of the fermentative degradation of carbohydrate increased the evolution of hydrocarbons under anaerobic, and to a greater extent, under aerobic conditions. In particular, ethanol and butyric acid promoted the formation of C2H4 and propylene, respectively. The association between degradation products and C2H4 suggests that both may be implicated when root growth is adversely affected by the anaerobic decomposition of plant residues. 相似文献
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A cutting experiment was carried out on a sward of S23 perennial ryegrass to study the effect of different defoliation regimes on regrowth and the factors affecting regrowth. The defoliation frequencies and intensities encompassed the range commonly found on continuously stocked pastures. The defoliation regimes produced swards of contrasting growth form with regrowth characteristics which differed in respect of senescence loss, fall in water-soluble carbohydrate concentration, residual leaf area index, photosynthesis per unit leaf area index and photosynthesis per unit area of land. Regrowth was most closely related to the latter. These results were used to interpret data for net canopy photosynthesis of continuously grazed pastures. 相似文献
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- 1 Climatic changes and water resource developments alter the relative contribution of the water sources (glacier-melt, snow-melt, rainfall run-off and springflow) to the discharge regime of alpine streams.
- 2 Likely changes in the magnitude and variability of a range of climatic determinants of glacier behaviour, as a result of an enhanced Greenhouse Effect, are shown to have important implications for the future hydrogeomorphological and thermal dynamics of alpine streams.
- 3 Zoobenthic communities typically show deterministic patterns in alpine streams due to the over-riding influence of water temperature and channel stability. These communities could act as indicators of change in the relative contribution of run-off, particularly to alterations of glacier-melt patterns.
- 4 A reduction of glacial meltwater, following diversion for HEP generation, is used as an example to illustrate the sensitivity of the zoobenthic communities to changes in temperature and flow regimes, and geomorphological dynamics.
10.
The epaulette shark (Hemiscyllium ocellatum) and the grey carpet shark (Chiloscyllium punctatum) are commonly found in periodically hypoxic environments. The ecophysiological time available for these animals to safely
exploit these niches during different seasonal temperatures was examined. The time to loss of righting reflex (T
LRR) was examined in response to an open ended anoxic challenge at three seasonal temperatures (23, 25 and 27°C). Ventilation
rates were measured in an open ended anoxic challenge at 23°C and during 1.5 h of anoxia followed by 2 h of re-oxygenation
at 23 and 25°C. The mean T
LRR of epaulette and grey carpet sharks was inversely proportional to temperature. The T
LRR was similar between species at 23°C; however, grey carpet sharks had significantly reduced T
LRR at higher temperatures. During the standardised anoxic challenge, epaulette sharks entered into ventilatory depression significantly
earlier at 25°C. During re-oxygenation, epaulette sharks exposed to anoxia at 23°C had no significant increase in ventilation
rates. However, after anoxic challenge and re-oxygenation at 25°C, epaulette sharks showed a significant increase in ventilation
rates during re-oxygenation. Grey carpet sharks displayed no evidence of ventilatory depression during anoxia. However, during
re-oxygenation, grey carpet sharks had significantly elevated ventilation rates above pre-experimental levels and control
animals. These data demonstrate that the anoxia tolerance times of both species were temperature dependent, with a significant
reduction in the T
LRR occurring at higher temperatures. Epaulette sharks had a significantly greater T
LRR at higher temperatures than grey carpet sharks, which did not enter into a ventilatory depression. 相似文献