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Landscape Ecology - Forest landscapes at the boreal–temperate ecotone have been extensively altered. Reducing the gap between current and presettlement forest conditions through...  相似文献   
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The direct exposure of fish eggs to ozonated water has generated interest as a means of ensuring pathogen-free eggs without the use of harsh chemicals. However, there are numerous knowledge gaps, including safe contact times, exposure levels, and potential long-term effects on aquaculture species in both freshwater and seawater. The effect of different ozone (O3) doses (0.5–1.0, 1.5–2.0, and 2.5–3.0 mg of O3/L for 90 s) on recently fertilized eggs of Atlantic Cod Gadus morhua and eyed eggs of Atlantic Salmon Salmo salar and Rainbow Trout Oncorhynchus mykiss was evaluated in comparison with the effects of two commercial disinfectants: Perosan (0.004 mg/L) and Ovadine (100 mg/L). The impact of ozone application was evaluated based on hatching success, larval nucleic acid concentration, larval growth, and survival. Overall, results indicated that ozonation of Atlantic Cod eggs at a dose less than 3.0 mg/L for 90 s produced no negative effect on the larvae up to 30 d posthatch. Furthermore, ozonation of Atlantic Salmon and Rainbow Trout eggs generated no negative effect on the larvae, based on monitoring until 85% yolk sac re-absorption (16 d posthatch).

Received May 6, 2014; accepted October 24, 2014  相似文献   

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Context

Sustained timber harvesting conflicts with the long-term viability of boreal caribou (Rangifer tarandus caribou) populations. The spatial arrangement of harvest blocks in the landscape could mitigate the impact of logging on caribou populations. For the forest industry, however, these measures represent constraints that reduce the annual allowable cut (AAC).

Objective

Estimate the long-term impacts of spatial constraints to harvesting, applied alone or in combination, on AAC and boreal caribou populations.

Methods

We divided a 30,000 km2 region into 20 harvest block sizes varying from 50 to 1000 km2, and modeled the implementation of spatially explicit harvest schedule plans in combination with wildfire and caribou population dynamics. We then evaluated the probability of persistence of boreal caribou populations.

Results

The probability of maintaining an AAC target declined with increasing target AAC, increasing size of operating area, and increasing adjacency constraints. In contrast, the probability of maintaining caribou populations declined with increasing AAC, decreasing size of operating areas, and decreasing adjacency constraints. An increase in operating area size from 50 to 300 km2 produced a considerable gain in AAC for all adjacency constraints.

Conclusions

Because adjacency constraints led only to a small increase in the probability of maintaining caribou populations, we recommend adopting less constraining landscape management actions, such as a 70-year period between two consecutive harvests in the same ~300-km2 operating area.
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Differential diagnoses for regurgitation and vomiting in dogs include diseases of the gastroesophageal junction. The purpose of this cross‐sectional study was to describe ultrasonographic characteristics of the abdominal esophagus and gastric cardia in normal dogs and dogs with clinical disease involving this region. A total of 126 dogs with no clinical signs of gastrointestinal disease and six dogs with clinical diseases involving the gastroesophageal junction were included. For seven euthanized dogs, ultrasonographic features were also compared with gross pathology and histopathology. Cardial and abdominal esophageal wall thicknesses were measured ultrasonographically for all normal dogs and effects of weight, sex, age, and stomach filling were tested. Five layers could be identified in normal esophageal and cardial walls. The inner esophageal layer was echogenic, corresponding to the cornified mucosa and glandular portion of the submucosa. The cardia was characterized by a thick muscularis, and a transitional zone between echogenic esophageal and hypoechoic gastric mucosal layers. Mean (±SD) cardial wall thicknesses for normal dogs were 7.6 mm (±1.6), 9.7 mm (±1.8), 10.8 mm (±1.6), 13.3 mm (±2.5) for dogs in the <10 kg, 10–19.9 kg, 20–29.9 kg and ≥30 kg weight groups, respectively. Mean (±SD) esophageal wall thicknesses were: 4.1 mm (±0.6), 5.1 mm (±1.3), 5.6 mm (±1), and 6.4 mm (±1.1) for the same weight groups, respectively. Measurements of wall thickness were significantly correlated with dog weight group. Ultrasonography assisted diagnosis in all six clinically affected dogs. Findings supported the use of transabdominal ultrasonography as a diagnostic test for dogs with suspected gastroesophageal disease.  相似文献   
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A field study was conducted to quantify spatial soil variability and to analyze correlations among soil properties at different spatial scales. Soil samples from 0 to 30 cm depth were collected from two adjacent fields in the southwestern Beauce Plain (France) which consisted of Haplic Calcisols and Rendzic Leptosols. Factorial kriging analysis (FKA) was used to describe the co-regionalization of nine soil properties. A linear model of co-regionalization including a nugget effect, and two spherical models were fitted to the experimental data direct and cross-variograms of the topsoil layer properties which were previously estimated. Co-kriged regionalized factors, related to short and long-range variation, were then mapped to characterize soil variation across the two fields. The potential value of ancillary sampled variables, such as yield data, to provide information on soil properties was tested. The relation between yield and measured soil properties appeared to be weak in general. However, the structures of the variation in yield appeared to be relatively stable for two years and showed similar patterns as the co-kriged soil factors. This suggests that information on the scale of variation of soil properties can be derived from yield maps, which can also be used as a guide to suitable sampling interval for soil properties and as a basis for managing fields in a precise way.  相似文献   
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The sensitivity and specificity of the indirect micro-enzyme-linked immunosorbent assay (ELISA) was compared with that of the rapid serum-plate test (RSPT) and the hemagglutination-inhibition test (HIT) in detecting antibodies to Mycoplasma gallisepticum (MG) and M. synoviae (MS). Membrane antigens of MG strain S6 and MS strain NEL 61800 were used. ELISA was performed with single MS and single MG antigens and a combined MS/MG antigen. The MS-ELISA was as sensitive as the MS-RSPT and more sensitive than and as specific as the MS-HIT in detecting antibodies to MS. The MG-ELISA was less sensitive than the MG-RSPT and slightly less sensitive than the MG-HIT in detecting antibodies to MG in chickens experimentally infected with MG R strain but more sensitive in detecting antibodies in chickens infected with MG F strain. MG-ELISA resulted in fewer cross-reactions than the MG-RSPT but more than the MG-HIT. The combined MG/MS-ELISA was as sensitive as the ELISA with its individual antigen components. No nonspecific reactions were observed with sera from MG/MS-free flocks. The combined MG/MS-ELISA was found to be a practical screening test for antibodies to both MS and MG. Further improvement of the sensitivity and the specificity of the MG antigen is desirable.  相似文献   
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