Emergence of Edwardsiella piscicida in Farmed Channel ♀, Ictalurus punctatus × Blue ♂, Ictalurus furcatus,Hybrid Catfish Cultured in Mississippi

There is a trend toward the increased incidence and prevalence of Edwardsiella piscicida septicemia in US catfish aquaculture, particularly in channel ♀, Ictalurus punctatus, × blue ♂, I. furcatus, hybrid catfish. From 2013 to 2017, a total of 3242 disease case submissions were made to the Aquatic Research and Diagnostic Laboratory (ARDL) at the Thad Cochran National Warmwater Aquaculture Center in Stoneville, MS. Of these, 1400 (43.2%) were hybrids. E. piscicida was suspected in 138 (4.3%) of cases, the majority of which (89.1%) were from hybrid catfish. A molecular survey of these isolates confirmed the majority (92.0%) to be E. piscicida. Furthermore, cases of E. piscicida from hybrids submitted to the ARDL and the Aquatic Diagnostic Laboratory of the Mississippi State University College of Veterinary Medicine in Starkville, MS, were documented for gross lesions and histological analysis. Grossly, E. piscicida presents with small dermal ulcerations, a raised fluid‐filled cranial midline lesion that is frequently ulcerated, hemorrhage in the gills, exophthalmia, and abdominal distension. Internally, lesions include splenomegaly, straw‐colored ascites, renomegaly, and occasionally hemorrhagic intestines. Histopathological examination is in agreement with gross observations, and infected fish repeatedly demonstrate a mononuclear meningoencephalitis, hemorrhagic branchitis, splenitis, ulcerative dermatitis, granulomatous interstitial nephritis, and hepatitis coupled with a hemorrhagic enteritis.     

NBA sweet spots: Distance‐based impacts on establishment‐level sales

This paper examines distance‐based effects of the introduction of a National Basketball Association (NBA) team on establishment‐level sales. Using a unique micro dataset with precise geographic location information and industry detail, we apply spatio‐temporal estimation strategies following Harger et al. and Ahlfeldt and Kavetsos. We build on the literature by focusing on sales activity, a broad measure of economic activity, for industries related to the NBA‐product. Our application considers the relocation of the NBA’s Seattle franchise (Supersonics) to Oklahoma City (Thunder). The results reveal spatially differentiated impacts that would be obscured using data aggregated over space or industry. Specifically, food establishments exhibit increased sales and entertainment establishments exhibit decreased sales relative to establishments in the outer most ring of the study area.     

Ascorbic acid changes the pattern of purine metabolism during germination of white spruce somatic embryos

It has previously been shown that exogenous applications of ascorbic acid (AA) increase the conversion frequency of somatic embryos of white spruce (Picea glauca (Moench) Voss). To determine whether ascorbic acid alters purine metabolism during the early phases of embryo germination, the relative rates of purine salvage and degradation were investigated by following the metabolic fates of exogenously applied [8-14C]adenine, [8-14C]adenosine, and [8-14C]inosine, and the activities of several key enzymes. We demonstrated that both the salvage and the degradation pathways operate during germination. Specifically, adenine and adenosine were mainly salvaged to nucleotides and nucleic acids, whereas an appreciable amount of inosine was degraded to CO2 and ureides. Comparisons of purine metabolism between control and AA-treated embryos showed that exogenous applications of ascorbic acid enhanced the ability of the embryos to take up adenine and adenosine throughout the germination period. Furthermore, the higher enzymatic activities of adenosine kinase and adenine phosphoribosyltransferase were responsible for the larger proportion of adenine and adenosine being salvaged in AA-treated embryos compared with control embryos. Thus, there was a positive correlation between the ability to anabolize purine precursors and successful embryo conversion.     

In vitro regeneration of Pacific silver fir (Abies amabilis) plantlets and histological analysis of shoot formation

A protocol is described for the in vitro propagation of Abies amabilis (Dougl.) Forbes. Over 60% of the cotyledonary explants from 5-day-old cotyledons formed shoots when cultured for 7 days on Schenk and Hildebrandt's (SH) medium containing 10 micro M N(6)-benzyladenine (BA) followed by another 7 days on SH medium containing 10 micro M each of BA and zeatin. Shoot multiplication was unsuccessful. Seventeen percent rooting was obtained after pulsing for 4 h in 1 mM indole-3-butyric acid and planting pulsed shoots in 1/1 (v/v) perlite/vermiculite. Cell clusters (promeristemoids) of five to seven cells were observed on the cotyledonary explants after 7 days of culture in the presence of cytokinin. These cells developed further into meristematic domes and apical meristems. In the absence of cytokinin, stomata and resin canals reached maturity, whereas cells within the cortex became vacuolated and developed into palisade and spongy mesophyll.     

Long-term ecology of native pinewood communities in East Glen Affric, Scotland

Fossil pollen, stomata and charcoal were examined from a lakesedimentary sequence in the Glen Affric National Nature Reserve,one of the largest areas of remnant native pine woodland inScotland, in order to assess ecosystem dynamics over the last11 000 years. Results reveal that pinewood communities havebeen continuously present in East Glen Affric for the last 8300years. Pinus sylvestris first arrived in the area around 9900cal. BP, but occurred in only low abundance for the subsequent1600 years. Pine populations expanded around 8300 cal. BP andremained in relatively constant abundance throughout the remainderof the Holocene. There is no evidence of a hypothesized regionalmid-Holocene ‘pine decline’ at the site. Charcoalresults reveal that pinewood communities in East Glen Affricdo not appear to have been dependent on fire for either theirestablishment or their maintenance as has previously been suggested.     

Mechanical site preparation effects on understory plant diversity in the Piedmont of the southern USA

Diversity of understory vegetation was compared among four intensities of site preparation and an adjacent 50-year-old pine-hardwood forest. The study site was a six-year-old loblolly pine (Pines taeda) plantation in the lower Piedmont of Georgia. Species richness and Shannon diversity indices were used to evaluate vine and woody (trees and shrubs) species diversity. Biomass distribution was compared among four plant categories: vines, forbs, grasses, and woody. Moderate intensity treatments (chainsaw and shear and chop) consistently ranked highest overall in diversity, with the mature pine-hardwood forest ranking lowest. Distribution of the four plant categories was not significantly affected by intensity of site preparation.Alabama Agricultural Experiment Station Series No. 9-892325P.     

At-sea distribution of waved albatrosses and the Galápagos Marine Reserve

Albatross populations worldwide are threatened by incidental takes in longline fishery operations. The recent establishment of the Galápagos Marine Reserve (GMR) is relevant to the longline bycatch issue, as it prohibits industrial longlining in the vicinity of the major nesting site of waved albatrosses (Phoebastria irrorata). However, the legality of the fishing protections is being challenged, highlighting a need for data on use of the GMR by albatrosses. We used satellite tracking over a total of four breeding seasons to determine the distribution of waved albatrosses inside and outside the GMR, and thereby assess the degree of protection that GMR provisions offer to this species. During the incubation period, breeding adults made commuting trips from the nesting island (Isla Española) to the Peruvian upwelling zone, traveling north, east, and south after leaving the nest. During the brooding period, the distribution contracted markedly, and most satellite fixes were within the GMR. During the rearing period, breeders performed both long trips outside the GMR and short trips within. The southeastern portion of the GMR is used throughout the incubation, brooding, and early rearing periods by breeding waved albatrosses. Indirect information from non-breeding adults indicates that they are likely to use the waters of the GMR extensively.     

In vivo investigation of changes in biomarkers of oxidative stress induced by plant food rich diets

It is well established that vegetables and fruit (VF) contain antioxidant phytochemicals. Consequently, it is expected that individuals who consume diets with a high content of VF should be better protected against oxidative cellular damage than individuals who do not, but not all data support this assumption. The objective of this study was to identify possible explanations for this conundrum. The effects of two diets that differed in VF content on markers of oxidative damage were studied. Sixty-four women participated in a 14-day dietary intervention. Subjects consumed on average either 3.6 or 12.1 servings of VF per day. The primary end points assessed were 8-hydroxy-2-deoxyguanosine (8-oxo-dG) in peripheral lymphocyte DNA and 8-isoprostaglandin F-2alpha (8-iso-PGF2alpha) excreted in urine. Subjects consuming the high versus low VF diet had lower concentrations of 8-oxo-dG (p < 0.01) and of 8-iso-PGF2alpha (p < 0.01). However, the reduction in oxidative end points by high VF was not uniform. Rather, an antioxidant effect was observed primarily in individuals whose oxidative end points at baseline were above the median for the study population. Using change in plasma carotenoids (end point minus baseline measurements) as an index of phytochemical intake, the reduction in oxidative markers was inversely proportional to change in plasma carotenoids; this effect was stronger for lipid peroxidation (p < 0.01) than DNA oxidation (p < 0.05). These findings imply that increasing exogenous antioxidant exposure may primarily benefit individuals with elevated levels of oxidative stress. Null findings do not necessarily indicate that an antioxidant compound lacks in vivo activity.