Reward-predictive cues enhance excitatory synaptic strength onto midbrain dopamine neurons

Using sensory information for the prediction of future events is essential for survival. Midbrain dopamine neurons are activated by environmental cues that predict rewards, but the cellular mechanisms that underlie this phenomenon remain elusive. We used in vivo voltammetry and in vitro patch-clamp electrophysiology to show that both dopamine release to reward predictive cues and enhanced synaptic strength onto dopamine neurons develop over the course of cue-reward learning. Increased synaptic strength was not observed after stable behavioral responding. Thus, enhanced synaptic strength onto dopamine neurons may act to facilitate the transformation of neutral environmental stimuli to salient reward-predictive cues.     

Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto

Helicobacter (H.) heilmannii sensu stricto (s.s.) is a zoonotic bacterium that naturally colonizes the stomach of dogs and cats. In humans, this microorganism has been associated with gastritis, peptic ulcer disease and mucosa associated lymphoid tissue (MALT) lymphoma. Little information is available about the pathogenesis of H. heilmannii s.s. infections in humans and it is unknown whether differences in virulence exist within this species. Therefore, a Mongolian gerbil model was used to study bacterium-host interactions of 9 H. heilmannii s.s. strains. The colonization ability of the strains, the intensity of gastritis and gene expression of various inflammatory cytokines in the stomach were determined at 9 weeks after experimental infection. The induction of an antrum-dominant chronic active gastritis with formation of lymphocytic aggregates was shown for 7 strains. High-level antral colonization was seen for 4 strains, while colonization of 4 other strains was more restricted and one strain was not detected in the stomach at 9 weeks post infection. All strains inducing a chronic active gastritis caused an up-regulation of the pro-inflammatory cytokine IL-1β in the antrum. A reduced antral expression of H⁺/K⁺ ATPase was seen in the stomach after infection with 3 highly colonizing strains and 2 highly colonizing strains caused an increased gastrin expression in the fundus. In none of the H. heilmannii s.s.-infected groups, IFN-γ expression was up-regulated. This study demonstrates diversity in bacterium-host interactions within the species H. heilmannii s.s. and that the pathogenesis of gastric infections with this microorganism is not identical to that of an H. pylori infection.     

Does shifting from conventional to zero tillage in combination with a cover crop offers opportunities for silage maize cultivation in Flanders?

Tillage is an important agricultural practice, influencing the physical, chemical and biological soil characteristics. In this paper the influence of various tillage systems combined with or without a cover crop under different nitrogen fertilization levels on silage maize yield and soil fertility was investigated. Based on a field trial in Bottelare (Belgium), during the period 2007–2015, it was concluded that for each tillage system higher nitrogen levels resulted in a higher yield. In addition, the highest yield was achieved for the conventional tillage system, the yield gain for mouldboard ploughing varied between 13% (2015) and 71% (2012) compared to zero tillage. In case reduced tillage was adopted, the yield loss compared to mouldboard ploughing varied between 6% (2013 and 2015) and 24% (2012). Furthermore, it seemed that the accumulated temperature during the growing season and rainfall around flowering were decisive in determining maize yield. Additionally, rainfall in the period 60 days post sowing was significantly negatively correlated with the yield from the zero tillage plots, whereas in case tillage was adopted no correlations with rainfall 60 days post sowing were detected. Concerning the soil organic carbon content and the amount of earthworms, no clear trends could be observed. Zero tillage resulted in high weed pressure and caused soil compaction. So, in this trial, under humid conditions, the less labor intensive zero tillage system did not result in competitive maize yields. In conclusion, reduced tillage methods offer opportunities for maize cultivation in Belgium. This method of farming resulted in a lower yield, however, the difference with mouldboard ploughing was not significant. Therefore, adopting a reduced tillage system can be seen as a valid alternative for ploughing as this tillage system ensures a sustainable environment.     

Fourteen years of applying zero and conventional tillage,crop rotation and residue management systems and its effect on physical and chemical soil quality

Soil management systems may negatively affect the quality of the soil. Policymakers and farmers need scientific information to make appropriate land management decisions. Conventional (CT) and zero tillage (ZT) are two common soil management systems. Comparative field studies under controlled conditions are required to determine the impact of these systems on soil quality and yields. The research presented studied plant and soil physical and chemical characteristics as affected by different agricultural management practices, i.e. ZT and CT, cropped with continuous wheat or maize in monoculture (M) or in a yearly rotation (R) of these two crops, either with residue retention (+r) or without residues retention (?r), in an experimental field in the Transvolcanic Belt of Mexico after 14 years. The dominant factors defining soil quality were organic C, total N, moisture, aggregate stability, mechanical resistance, pH and EC. The principal component combining the variables organic C, total N, aggregate stability and moisture content showed the highest correlations with final yield (R = 0.85 for wheat and 0.87 for maize).After 14 years of continuous practice, ZTM + r and ZTR + r had the best soil quality and produced the highest wheat and maize yields of average 2001–2004 (6683 and 7672 kg ha^?1 and 5085 and 5667 kg ha^?1, respectively). Removing the residues, i.e. treatments ZTM ? r with maize (average 2001–2004: 1388 kg ha^?1) and ZTR ? r and CTR ? r with wheat (average 2001–2004: 3949 and 5121 kg ha^?1), gave the lowest yields and less favourable soil physical and chemical characteristics compared to the other practices. It was found that zero tillage with residue retention is a feasible management technology for farmers producing maize and wheat in the agro-ecological zone studied, resulting in a better soil quality and higher yields than with the conventional farmer practice (maize monoculture, conventional tillage and residue removal).     

Straw management, crop rotation, and nitrogen source effect on wheat grain yield and nitrogen use efficiency

Nitrogen fertilizer management from different sources and annual crop rotations are important components of wheat (Triticum aestivum L.) production systems, especially where air and soil quality issues have prompted a search for alternatives to wheat straw burning. This study examined the effects of two different wheat straw management options (burning and incorporation by tillage), three crop rotations [wheat-sesbania (Sesbania spp.), wheat–maize (Zea mays L.), and wheat-clean fallow] and three N sources (urea, chicken manure, and urea plus chicken manure) on wheat grain yield and N use efficiency. The experiment was conducted as split–split plot treatment arrangement with three replications for eight wheat cropping seasons in the state of Sonora, Mexico. Results indicated that both wheat grain yield and N use efficiency were higher with burning than incorporation of wheat straw and with fertilization with urea or urea plus chicken manure than chicken manure alone. As shown by the crop rotation-by-straw management interaction, planting sesbania following incorporation of the straw by tillage produced comparable grain yields to straw burning treatment. In contrast, wheat in annual rotation with maize produced the lowest wheat grain yield and N use efficiency irrespective of the wheat straw management and N source applied.     

Effects of large-scale wetland loss on network connectivity of the Rainwater Basin,Nebraska

Context

The Rainwater Basin region in south-central Nebraska supports a complex network of spatially-isolated wetlands that harbor diverse floral and faunal communities. Since European settlement, many wetlands have been lost from the network, which has increased distances among remaining wetlands. As a result, populations of wildlife species with limited dispersal capabilities may have become isolated and face greater local extinction risks.

Objectives

We compared the pre-European settlement and current extent of the Rainwater Basin network to assess the effects of wetland losses on network connectivity for a range of maximum dispersal distances.

Methods

We constructed network models for a range of maximum dispersal distances and calculated network metrics to assess changes in network connectivity and the relative importance of individual wetlands in regulating flow.

Results

Since European settlement, the number of wetlands in the Rainwater Basin has decreased by?>?90%. The average distance to the nearest neighboring wetland has increased by 150% to ~?1.2 km, and the dispersal distance necessary to travel throughout the whole network has increased from 3.5 to 10.0 km. Last, relative importance of individual wetlands depended on the maximum dispersal distance. Which wetlands to preserve to maintain connectivity might therefore depend on the dispersal capabilities of the species or taxa of interest.

Conclusions

To preserve a broad range of biodiversity, conservation efforts should focus on preserving dense clusters of wetlands at fine spatial scales to maintain current levels of network connectivity, and restoring connections between clusters to facilitate long-range dispersal of species with limited dispersal capabilities.

     

以农民经验小组方法评价奶牛饲料的应用价值

采用优劣势分析和配对排序法,分析了玉米秸作为粗饲料饲喂产奶牛的优缺点.奶农采用干玉米秸杆做为饲草资源, 容易得到、饲喂成本低是主要原因,容易贮存制备、提供粗纤维是次要原因;营养价值低、不利于繁殖性能和产奶量提高是制约因素.采用半结构性访谈,以奶农经验小组评价了”种植高-丹草饲养奶牛”和”夏季对奶牛补饲保护性脂肪”在奶牛生产中的适用性.在耕地很少的城市郊区,二者分别居于农民需求程度的第3位和第2位.高-丹草种植,宜早于6月,行距应宽于50 cm.补饲保护性脂肪,使奶牛抗热性增强,产奶量稳定,乳脂率提高明显.     

Colonization strategy of Campylobacter jejuni results in persistent infection of the chicken gut

Although poultry meat is now recognized as the main source of Campylobacter jejuni gastroenteritis, little is known about the strategy used by the bacterium to colonize the chicken intestinal tract. In this study, the mechanism of C. jejuni colonization in chickens was studied using four human and four poultry isolates of C. jejuni. The C. jejuni strains were able to invade chicken primary cecal epithelial crypt cells in a predominantly microtubule-dependent way (five out of eight strains). Invasion of cecal epithelial cells was not accompanied by necrosis or apoptosis in the cell cultures, nor by intestinal inflammation in a cecal loop model. C. jejuni from human origin displayed a similar invasive profile compared to the poultry isolates. Invasiveness of the strains in vitro correlated with the magnitude of spleen colonization in C. jejuni inoculated chicks. The C. jejuni bacteria that invaded the epithelial cells were not able to proliferate intracellularly, but quickly evaded from the cells. In contrast, the C. jejuni strains were capable of replication in chicken intestinal mucus. These findings suggest a novel colonization mechanism by escaping rapid mucosal clearance through short-term epithelial invasion and evasion, combined with fast replication in the mucus.     

A minimum data set for soil quality assessment of wheat and maize cropping in the highlands of Mexico

The objective was to establish a minimum soil quality dataset for a long-term tillage, residue management and rotation trial for wheat (Triticum aestivum L.) and maize (Zea mays L.) production systems. Based on this soil quality evaluation, sustainable management practices could be selected for transferring technologies to farmers in the region. A long-term experiment was conducted with 16 different crop management practices varying in: (1) rotation (continuous maize or wheat and both phases of the rotation of maize and wheat), (2) tillage (conventional and zero) and (3) crop residue management (full retention or removal for fodder). Superior soil quality was considered to represent the maintenance of high productivity without significant soil or environmental degradation. The pertinent, minimum soil quality data set included the following physical indicators: time-to-pond, aggregate stability, permanent wilting point, and topsoil penetration resistance. Chemical indicators were: soil C, N, K and Zn concentrations, measured in the 0–5 cm topsoil and C, N concentration in 5–20 cm. Multivariate analysis grouped the treatments into clusters: (1) zero tillage with retention of residue, (2) zero tillage with residue removal and (3) conventional tillage. Zero tillage combined with crop residue retention improved chemical and physical conditions of the soil. In contrast, zero tillage with removal of residues, led to high accumulation of Mn in the topsoil, low aggregate stability, high penetration resistance, surface slaking resulting in low time-to-pond values and high runoff. Finally, soil quality under conventional tillage was intermediate (irrespective of residue management), especially reflected in the physical status of the soil. The results provide a strong justification to promote zero tillage technology combined with appropriate residue management to farmers in the volcanic highlands of Central Mexico and other similar regions. The minimum data set and associated tools for careful monitoring and observation, will be essential for evaluating soil quality in farmer's fields.