Habitat associations of the Threatened pugnose minnow (Opsopoeodus emiliae) at the northern edge of the species range

Pugnose minnow (Opsopoeodus emiliae) is a small, reclusive species that is widespread in North America, but is one of the rarest fishes in Canada, found in less than 12 known localities in southwestern Ontario. In contrast to most pugnose minnow populations across the global range, Canadian populations are primarily found in turbid systems, potentially indicating persistence in suboptimal conditions. We used data from a multi-gear species and habitat survey in the Canard River, Ontario, a system dominated by agricultural inputs and the best-known capture site of the species in Canada, to parameterise multi-gear occupancy models for understanding the relationship between pugnose minnow occupancy and microhabitat features, including the role of turbidity. Almost 300 pugnose minnow were captured, representing the largest single collection of the species in Canadian history. The best occupancy model indicated that the probability of pugnose minnow occupancy was highest in the deepest sites with the lowest water clarity (i.e. high turbidity); however, competing models suggested that occupancy was highest at sites with wild celery (Vallisneria americana) and higher water clarity, signifying that habitats with low turbidity may be utilised if sufficient physical cover exists. Together, our results suggest that Canadian pugnose minnow populations occupy and potentially favour turbid conditions, possibly to avoid visual predators in clearer habitats. It remains uncertain whether this abiotic association represents a long-term, viable, local adaptation or whether persistence of pugnose minnow in the Canard River is at risk unless significant water quality improvements can be made.     

Dietary nucleotides enhance growth performance,feed efficiency and intestinal functional topography in European Seabass (Dicentrarchus labrax)

Nucleotides, nucleosides and nucleic acids (NU) have many critical functions in supporting life and increasing evidence suggests that exogenous supply can benefit the health of mammals and fish. For these reasons, a 6‐week feeding trial was conducted on juvenile European seabass (Dicentrarchus labrax) with diets containing 0%, 0.15% and 0.3% inclusion of a NU mixture (Laltide^®) derived from the yeast Saccharomyces cerevisiae. At the end of the study no significant differences were found in fish performance, although a tendency towards better performance was indicated in fish fed the Nu0.3 diet. In relation to histological assessment, a significantly greater perimeter ratio; internal to outer (IP/OP) was observed in the posterior intestine of fish fed supplemental NU. Microvilli heights in the posterior intestine were also shown to be significantly promoted in fish fed NU diets (p < 0.05). Goblet cell abundance was shown to be unaffected by the inclusion of NU in the diet (p > 0.05). Overall, this study indicates that orally administered NU may be effective promoters of gut functional topography with marginal associated improvements to fish performance. Nonetheless, longer exposure and/or commercial scale application, and in diets that were challenging in use of high inclusion levels of plant by‐products would potentially amplify improvements in production characteristics, in turn benefiting fish culturists.     

Historical patterns of fire severity and forest structure and composition in a landscape structured by frequent large fires: Pumice Plateau ecoregion,Oregon, USA

Context

Lack of quantitative observations of extent, frequency, and severity of large historical fires constrains awareness of departure of contemporary conditions from those that demonstrated resistance and resilience to frequent fire and recurring drought.

Objectives

Compare historical and contemporary fire and forest conditions for a dry forest landscape with few barriers to fire spread.

Methods

Quantify differences in (1) historical (1700–1918) and contemporary (1985–2015) fire extent, fire rotation, and stand-replacing fire and (2) historical (1914–1924) and contemporary (2012) forest structure and composition. Data include 85,750-ha tree-ring reconstruction of fire frequency and extent; >?375,000-ha timber inventory following >?78,900-ha fires in 1918; and remotely-sensed maps of contemporary fire effects and forest conditions.

Results

Historically, fires?>?20,000 ha occurred every 9.5 years; fire rotation was 14.9 years; seven fires?>?40,469 ha occurred during extreme drought (PDSI <?? 4.0); and stand-replacing fire occurred primarily in lodgepole (Pinus contorta var. murrayana). In contemporary fires, only 5% of the ecoregion burned in 30 years, and stand-replacing fire occurred primarily in ponderosa (Pinus ponderosa) and mixed-conifer. Historically, density of conifers?>?15 cm dbh exceeded 120 trees/ha on?<?5% of the area compared to 95% currently.

Conclusions

Frequent, large, low-severity fires historically maintained open-canopy ponderosa and mixed-conifer forests in which large fire- and drought-tolerant trees were prevalent. Stand-replacing patches in ponderosa and mixed-conifer were rare, even in fires >?40,469 ha (minimum size of contemporary “megafires”) during extreme drought. In this frequent-fire landscape, mixed-severity fire historically influenced lodgepole and adjacent forests. Lack of large, frequent, low-severity fires degrades contemporary forest ecosystems.

     

Simulation modelling of potato virus Y spread in relation to initial inoculum and vector activity

Potato virus Y(PVY) is a non-persistent virus that is transmitted by many aphid species and causes significant damage to potato production. We constructed a spatially-explicit model simulating PVY spread in a potato field and used it to investigate possible effects of transmission efficiency, initial inoculum levels, vector behavior, vector abundance, and timing of peak vector activity on PVY incidence at the end of a simulated growing season. Lower PVY incidence in planted seed resulted in lower virus infection at the end of the season. However, when populations of efficient PVY vectors were high, significant PVY spread occurred even when initial virus inoculum was low. Non-colonizing aphids were more important for PVY spread compared to colonizing aphids, particularly at high densities. An early-season peak in the numbers of noncolonizing aphids resulted in the highest number of infected plants in the end of the season, while mid-and late-season peaks caused relatively little virus spread. Our results highlight the importance of integrating different techniques to prevent the number of PVY-infected plants from exceeding economically acceptable levels instead of trying to control aphids within potato fields. Such management plans should be implemented very early in a growing season.     

Macroalgal meadow habitats support fish and fisheries in diverse tropical seascapes

Canopy‐forming macroalgae can construct extensive meadow habitats in tropical seascapes occupied by fishes that span a diversity of taxa, life‐history stages and ecological roles. Our synthesis assessed whether these tropical macroalgal habitats have unique fish assemblages, provide fish nurseries and support local fisheries. We also applied a meta‐analysis of independent surveys across 23 tropical reef locations in 11 countries to examine how macroalgal canopy condition is related to the abundance of macroalgal‐associated fishes. Over 627 fish species were documented in tropical macroalgal meadows, with 218 of these taxa exhibiting higher local abundance within this habitat (cf. nearby coral reef) during at least one life‐history stage. Major overlap (40%–43%) in local fish species richness among macroalgal and seagrass or coral reef habitats suggest macroalgal meadows may provide an important habitat refuge. Moreover, the prominence of juvenile fishes suggests macroalgal meadows facilitate the triphasic life cycle of many fishes occupying diverse tropical seascapes. Correlations between macroalgal canopy structure and juvenile abundance suggests macroalgal habitat condition can influence levels of replenishment in tropical fish populations, including the majority of macroalgal‐associated fishes that are targeted by commercial, subsistence or recreational fisheries. While many macroalgal‐associated fishery species are of minor commercial value, their local importance for food and livelihood security can be substantial (e.g. up to 60% of landings in Kenyan reef fisheries). Given that macroalgal canopy condition can vary substantially with sea temperature, there is a high likelihood that climate change will impact macroalgal‐associated fish and fisheries.     

The release and uptake of metals from potential biofilm inhibition products during spiny lobster (Sagmariasus verreauxi,H. Milne Edwards 1851) culture

Zinc (Zn) and copper (Cu) are strong inhibitors of bacterial biofilms in aqueous solutions, but are known toxins of crustaceans. A new metal application method; cold‐sprayed metal embedment, known to modulate metal release, was tested for its applications in crustacean larval culture systems. Cold‐spray technology allows metal particles to bond to plastics, while modulating metal ion release and biocide activity to the substrate boundary. In this study, Eastern spiny lobster (Sagmariasus verreauxi) larvae (phyllosoma) were cultured in the presence of cold‐sprayed Zn and Cu metal surfaces. Metal loss was monitored gravimetrically on embedded surfaces, assessment of water ion concentrations and analysis of phyllosoma body content were undertaken. Phyllosoma moulting, deformity and mortality patterns were monitored. Cold‐sprayed Zn‐ and Cu‐embedded surfaces were depleted with losses of 0.69% and 31.2% noted respectively. Culture water concentrations of these metals were elevated and accumulation by phyllosoma occurred. Water Zn concentrations of 18.5 μg L^?1 were associated with chronic eyestalk moult deformities; the first report of Zn causing a non‐lethal moult deformity in crustacean larvae. The Cu surface lost a third of its metal mass with a water concentration of 40 μg L^?1 causing acute toxicity and localization of composite granules in the midgut gland. Cu associated mortality was noted by Day 2 of culture with a LD 50 experienced by Day 9. Future work on the use of bioactive metals in aquaculture systems will focus on a range of different metal alloys, and improved modulation of ion release mechanisms through increased particle embedment depth and separation.     

Seasonal movements of immature Kemp's ridley sea turtles (Lepidochelys kempii) in the northern gulf of Mexico

1. Seasonal movements and core habitat areas of immature Kemp's ridley sea turtles (Lepidochelys kempii) in the northern Gulf of Mexico were tracked via satellite telemetry. Tagged turtles were incidentally captured by recreational fishermen and rehabilitated at the Institute for Marine Mammal Studies in Gulfport, Mississippi.
2. The average size of the core habitat areas (50% KDE (kernel density estimation)) was 1660.2 km² ± 3438.2 SD. Turtles displayed strong intra‐ and inter‐annual site fidelity to the Mississippi Sound during the spring, summer, and autumn months. During the winter months, most turtles, probably influenced by water temperatures, migrated to nearshore waters of Louisiana on either side of the Mississippi River Delta. However, other migration strategies were also observed.
3. Overall, these data indicate that the Mississippi Sound is an important developmental habitat for this critically endangered species. In addition, their wintering grounds in Louisiana are utilized by adult Kemp's ridleys and other sea turtle species as foraging grounds and migratory corridors. The high use of these areas by sea turtle populations increases the potential for negative impacts from anthropogenic disturbances (e.g. shrimp trawling, oil production, hypoxia) that occur there.

Copyright © 2016 John Wiley & Sons, Ltd.     

Cohort‐specific variation in juvenile coho salmon habitat use

In this study, we examined summer and fall freshwater rearing habitat use by juvenile coho salmon (Oncorhynchus kisutch) in the quickly urbanising Big Lake drainage in south‐central Alaska. Habitat use was assessed by regressing fish count data against habitat survey information across thirty study sites using generalised linear mixed models. Habitat associations were examined by age‐0 and age‐1+ cohorts separately, providing an opportunity to compare habitat use across different juvenile coho salmon life stages during freshwater rearing. Regression results indicated that the age‐0 cohorts were strongly associated with shallow, wide stream reaches with in‐stream vegetation, whereas age‐1+ cohorts were associated with deeper stream reaches. Furthermore, associations between fork length and habitat characteristics suggest cohort‐specific habitat use patterns are distinct from those attributable to fish size. Habitat use information generated from this study is being used to guide optimal fish passage restoration planning in the Big Lake drainage. Evidence for habitat use partitioning by age cohort during freshwater juvenile rearing indicates that pooling age cohorts into a single “juvenile” stage for the purposes of watershed management may mask important habitat use dynamics.     

Shrimp (Litopenaeus vannamei) production and stable isotope dynamics in clear‐water recirculating aquaculture systems versus biofloc systems

Closed recirculating aquaculture systems (RAS) offer advantages over traditional culture methods including enhanced biosecurity, the possibility of indoor, inland culture of marine species year‐round and potential marketing opportunities for fresh, never‐frozen seafood. Questions still remain regarding what type of aquaculture system may be best suited for the closed‐system culture of marine shrimp. In this study, shrimp (Litopenaeus vannamei) were grown in clear‐water RAS and in biofloc‐based systems. Comparisons were made between the system types with respect to water quality, shrimp production and stable isotope dynamics used to determine the biofloc contribution to shrimp nutrition. Ammonia and nitrite concentrations were higher, and shrimp survival was lower in the biofloc systems. Although stable isotope levels indicated that biofloc material may have contributed 28% of the carbon and 59% of the nitrogen in shrimp tissues, this did not correspond with improved shrimp production. Overall, the water column microbial communities in biofloc systems may be more difficult to manage than clear‐water RAS which have external filters to control water quality. Biofloc does seem to offer some nutritional contributions, but exactly how to take advantage of that and ensure improved production remains unclear.