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During fertilization in mammalian species, a sperm-induced intracellular Ca2+ signal ([Ca2+]i) mediates both exit of meiosis and oocyte activation. Recently, we demonstrated in mouse oocytes that the phosphorylation levels of inositol 1,4,5 trisphosphate receptor type1 (IP3R1), the channel responsible for Ca2+ release and oscillations during fertilization, changed during maturation and fertilization. Therefore, we examined the expression and phosphorylation of IP3R1 during in vitro maturation of pig oocytes. Here, our present study shows that expression of IP3R1 protein did not change during maturation, although the phosphorylation status of the receptor, specifically at an MPM-2 epitope, did. We found that while at the beginning of maturation IP3R1 lacked MPM-2 immunoreactivity, it became MPM-2 reactive by 24 h and reached maximal reactivity by 36 h. Interestingly, the acquisition of MPM-2 reactivity coincided with the activation of p34cdc2 kinase and mitogen-activated protein kinase (MAPK), which are involved in meiotic progression. Following completion of maturation, inactivation of MAPK by U0126 did not affect IP3R1 phosphorylation, although inactivation of p34cdc2 kinase by roscovitine dramatically reduced IP3R1 phosphorylation. Neither inhibitor affected total expression of IP3R1. Altogether, our results show that IP3R1 undergoes dynamic phosphorylation during maturation and this might underlie the generation of oscillations at fertilization.  相似文献   
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为了开发哺乳动物胚胎的室温保存液 ,在室温 ( 2 0℃ )条件下 ,用磷酸缓冲型 PB1、HEPES缓冲型 M2及重碳酸缓冲型 m KRB等 3种溶液及其修正液 ,保存小鼠桑椹胚 4 8h或 72 h,然后经体外培养 ,观察其发育至扩大囊胚的能力。结果 ,用 M2液保存的小鼠桑椹胚获得比较高的囊胚率 ;从 M2液中除去 Na H2 PO4、Na HCO3及葡萄糖 3种成分保存的小鼠桑椹胚 ,发育至扩大囊胚的能力显著提高 ;从溶液中除去丙酮酸钠和乳酸钠 ,或者添加 EDTA和谷氨酰胺 ,对保存后的桑椹胚发育至扩大囊胚能力无显著影响 ;而在保存液中添加犊牛血清后 ,小鼠胚胎的发育能力反而下降。结果表明 ,在室温条件下 ,不含 Na H2 PO4、Na HCO3及葡萄糖 3种成分的 M2液 ,对保存小鼠桑椹胚非常有效  相似文献   
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We have numerically modeled the advection and diffusion of sardine eggs and larvae to investigate the larval transport processes of Japanese sardine from the spawning grounds by the Kuroshio.
The results indicated that the offshore drift current induced by the winter monsoon and the location of the spawning ground have significant effects on the survival of the Japanese sardine. The contribution of the drift current, the distance of the spawning ground from the Kuroshio axis, and the eddy diffusivity to the larval retention in the coastal area is approximately expressed by the following equation: where R is the retention rate in the coastal area, a the variance of initial distribution of eggs, T the time after the eggs were spawned, – V0 the velocity of the wind-induced offshore current, y0 the distance of the center of the spawning area from the Kuroshio axis, and K the coefficient of horizontal eddy diffusivity.
The year-to-year variation in larval survival rates stimulated by the two-dimensional model are consistent with those estimated previously by using field data of egg and larval abundance during 1978–1988.  相似文献   
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The spatial distribution and seasonal variability of epipelagic chaetognaths along the Kurile Islands and off south-east Hokkaido, in the western subarctic Pacific Ocean. were investigated during the period from May 1990 to October 1992. Sagitta elegans was the dominant species among the epipelagic chaetognaths in the study area. Juvenile S. elegans were distributed mainly along the path of the mixed water which was determined by the acceleration potential anomaly (APA) at the iso-pycnal density surface of 26.6 σθ. The location of the path of The mixed water meandered around cyclonic and anticyclonic eddies. Catches of S. elegans were made at temperatures ranging from 4.5 to 22.2C. The juvenile distribution, however, was mostly restricted to between 1 and 4C of the potential temperature determined at the isopycnal density surface of 26.6 σθ. Variability in the juvenile abundance within the path of the mixed water could be caused by predation rather than by food limitation because microzooplankton, which are known to he prey for juveniles, were abundant enough to meet the food requirements of juveniles in the study area. Adults occurred in spring (April-May) and young individuals (juvenile and stage 1) were abundant in summer (June-July), when a strong thermocline developed. The main spawning period appears to be during April-May, with a possible second spawning period in the autumn.  相似文献   
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The spring bloom of phytoplankton is a well-established, regular, seasonal event in the western subarctic Pacific and is considered one of the most important conditions of massive production of pelagic fishes. A series of 12 cruises was conducted from 1990 to 1992 to examine the timing and magnitude of the spring phytoplankton bloom in the Oyashio region, the western subarctic Pacific off Hokkaido, Japan. An interannual variability in the bloom events was also analysed. On the basis of hydrographical characteristics, the study area was divided into three water masses: the Oyashio Water Mass, the Mixed Water Mass, and the Coastal Water Mass. Spring blooms were observed first in April in the Oyashio and the Coastal Water Masses, and continued to May in 1991 and 1992. However, no bloom was recorded in the Mixed Water Mass. High nutrient supply into the surface mixed layer during winter is likely to be one of the factors supporting an intense spring bloom in the Oyashio Water Mass. A significant positive relationship between log-transformed surface chlorophyll a concentration and maximum density gradient (MDG) within the euphotic layer was obtained in April, indicating the importance of vertical stability of the water column in the initiation of spring blooms in the Oyashio and the Coastal Water Masses. The spring blooms in 1991 were much more extensive and lasted longer than in 1990. It is suggested that meteorological conditions and abundance of grazers were responsible for this interannual difference.  相似文献   
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Fish oocytes have not been cryopreserved successfully, probably because it is difficult to prevent intracellular ice from forming. Previously, we have shown in medaka that immature oocytes are more suitable for cryopreservation than mature oocytes or embryos, in terms of permeability. We have also shown in immature medaka oocytes that the exogenous expression of aquaporin 3 (AQP3), a water/cryoprotectant channel, promotes the movement of water and cryoprotectants through the plasma membrane. In the present study, we attempted to cryopreserve immature medaka oocytes expressing AQP3. We first examined effects of hypertonic stress and the chemical toxicity of cryoprotectants on the survival of the AQP3-expressing oocytes. Exposure to hypertonic solutions containing sucrose decreased the survival of oocytes, but the expression of AQP3 did not affect sensitivity to hypertonic stress. Also, AQP3 expression did not markedly increase sensitivity to the toxicity of cryoprotectants. Of the four cryoprotectants tested, propylene glycol was the least toxic. Using a propylene glycol-based solution, therefore, we tried to cryopreserve immature oocytes by vitrification. During cooling with liquid nitrogen, all intact oocytes became opaque, but many AQP3-expressing oocytes remained transparent. This indicates that the expression of AQP3 is effective in preventing intracellular ice from forming during cooling. During warming, however, all the AQP3-expressing oocytes became opaque, indicating that intracellular ice formed. Therefore, the dehydration and permeation by propylene glycol were still insufficient. Further studies are necessary to realize the cryopreservation of fish oocytes.  相似文献   
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To obtain blood biochemical basic data of Japanese Black calves in Kagoshima Prefecture, Japan, blood samples were obtained from 582 clinically healthy calves on 27 farms. Calves were divided into three stages: the suckling stage (between 14 and 90 days of age, n=191), the early growing stage (between 91 and 180 days of age, n=200) and the late growing stage (between 181 and 270 days of age, n=191). The mean concentration of total cholesterol, triglyceride, nonesterified fatty acids, calcium and zinc, and the mean activities of γ-glutamyltransferase and alkaine phospatase in the suckling stage were significantly higher than those in the early and late growing stages (P<0.01). The mean concentration of total protein, albumin and globulin increased gradually with growing. The mean concentration of beta-hydroxybutyrate in the suckling stage was below 150 µmol/l, however, it elevated above 400 µmol/l in the early and late growing stages. The mean concentration of copper concentration was above 70 µg/dl in all stages. The mean concentration of zinc was between 90 and 110 µg/dl in all stages. These results suggest that the blood biochemical values of Japanese Black calves vary with growing stages, and the blood parameters obtained in this study are considered useful as indices for health management of Japanese Black calves.  相似文献   
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