Influence of fruit ripeness at the time of seed extraction on pepper (Capsicum annuum) seed germination

The influence of fruit ripeness (half ripe, fully ripe, overripe) at the time of seed extraction on seed germination behaviour, at 25 °C and 13 °C, was studied in two Spanish pepper cultivars for canning. Seeds from half ripe fruits had a poorer behaviour than those taken from fully ripe fruits, especially at 13 °C. Room ripening and overripening of half ripe fruits improved the germination behaviour of the seeds although this behaviour was poorer than that of seeds extracted from fully ripe fruits that were allowed to overripen. Overripening of fully ripe picked fruits slightly improved germination behaviour.     

Epilithic biofilm as a key factor for small‐scale river fisheries on Caribbean islands

Abstract Numerous freshwater species are consumed or exploited through artisanal fisheries in the rivers of the islands of Guadeloupe, French West Indies. Autochthonous production of organic matter is limited in these flowing rivers and is mainly represented by scarce filamentous green algae and an abundant epilithic biofilm growing on wet stones in the river bed. Stable isotope analysis was used to quantify the relative importance of biofilm and other riverine allochthonous and autochthonous food sources in the diet of tropical shrimps (Palaemonidae, Atyidae and Xiphocarididae) and fishes (Gobiidae and Eleotridae) consumed by the local people. The epilithic biofilm was exploited by most species, constituted an important source of autochthonous carbon and was an important source of organic matter production at the base of freshwater food webs in Caribbean rivers. Biofilm percentages in the diet reached 32% for molluscs, 85% for atyid shrimps, 29% for xiphocaridid shrimps, 14% for palaemonid shrimps and 13% for fish. Assessment of biofilm in nine rivers showed that blue and red cyanobacteria were quantitatively dominant with a moderately rich diatom flora. These results address the interactions between river biofilm and Caribbean freshwater fauna where trophic links between consumers and their potential resources have poorly been documented.     

新《兽药管理条例》实施过程中存在的难点

介绍了新<兽药管理条例>在当前兽药管理实践中存在的难点和问题,提出了解决这些问题的建议和措施.     

Wind effects on solid set sprinkler irrigation depth and yield of maize (<Emphasis Type="Italic">Zea mays</Emphasis>)

A field experiment was performed to study the effect of the space and time variability of water application on maize (Zea mays) yield when irrigated by a solid set sprinkler system. A solid set sprinkler irrigation layout, typical of the new irrigation developments in the Ebro basin of Spain, was considered. Analyses were performed (1) to study the variability of the water application depth in each irrigation event and in the seasonal irrigation and (2) to relate the spatial variability in crop yield to the variability of the applied irrigation and to the soil physical properties. The results of this research showed that a significant part of the variability in the Christiansen coefficient of uniformity (CU), and wind drift and evaporation losses were explained by the wind speed alone. Seasonal irrigation uniformity (CU of 88%) was higher than the average uniformity of the individual irrigation events (CU of 80%). The uniformity of soil water recharge was lower than the irrigation uniformity, and the relationship between both variables was statistically significant. Results indicated that grain yield variability was partly dictated by the water deficit resulting from the non-uniformity of water distribution during the crop season. The spatial variability of irrigation water depth when the wind speed was higher than 2 m s^–1 was correlated with the spatial variability of grain yield, indicating that a proper selection of the wind conditions is required in order to attain high yield in sprinkler-irrigated maize.     

A database program for enhancing irrigation district management in the Ebro Valley (Spain)

In the last decade irrigation districts in the Ebro Valley of Spain have started to use database applications to enhance their management operations. Such applications often put more emphasis on administrative issues than on water management issues. A new irrigation district management software called “Ador” is presented in this paper. This database application has been designed to overcome limitations identified in an analysis of the software used in the study area. Ador can be used in irrigation districts independently of the type of irrigation system (surface, sprinkler or trickle) and the type of irrigation distribution network (open channel or pressurised). It can even be used in irrigation districts combining different types of irrigation systems and different types of irrigation distribution networks. The software can be used with minimum district information. The goals are to manage detailed information about district water management and to promote better on-farm irrigation practices. Ador is currently used to enhance management of 62 irrigation districts accounting for some 173,000 hectares in the Ebro Valley.     

Isolation of antioxidant compounds from orange juice by using countercurrent supercritical fluid extraction (CC-SFE).

Antioxidants from orange juice are isolated by the use of countercurrent supercritical fluid extraction (CC-SFE) and characterized by reversed-phase liquid chromatography (RPLC) coupled to mass spectrometry (MS) and diode-array detection (DAD). A pilot-scale SFE plant equipped with a packed column has been employed for countercurrent extraction and fractionation of raw orange juice with carbon dioxide. Several experiments have been performed in order to study the effect of the countercurrent conditions on the content of antioxidative compounds. In this study, the main variable that has been considered is the solvent-to-feed ratio (S/F) because it plays an essential role in the extraction efficiency. The values tested covered a wide range of sample and solvent (CO(2)) flow rates. In each experimental run, two different extracted fractions and the residual nonextracted juice were obtained and characterized. Different flavonoids have been identified in the fractions obtained after CC-SFE. The possibility of using this process for antioxidant compounds enrichment is discussed.     

Pirarucu growth under different feeding regimes

In order to assess the most suitable feeding schedule for pirarucu, Arapaima gigas, we placed groups of seven fish in 12 1-m³ cages and investigated various feeding variables in a 2 × 2 factorial design. The feeding variables analyzed were one feeding (OC) or two feedings (TW), feeding daily (1D) or every other day (2D), and their interactions: one feeding once per day (OC/1D), two feedings per day (TW/1D), one feeding every other day (OC/2D) and two feedings every other day (TW/2D). The experiment lasted for 45 days, and the fish were maintained on a commercial extruded diet. The results showed similar final weights between fish maintained on feedings OC and TW, but higher ones in fish fed 1D than 2D. Weight gain was higher when the fish were fed TW and 1D. Apparent feed conversion was not affected in fish fed OC or TW and 1D or 2D. Daily feed consumption was also higher for the feeding schedules of TW and 1D. The combination of feeding schedules produced higher final weights and weight gains for fish fed OC/1D and TW/1D. The apparent feed conversion value was higher only in fish fed TW/1D. Daily feed consumption values were significantly higher in fishes fed TW/1D. The lowest value was recorded for OC/2D.     

Seasonal on-farm irrigation performance in the Ebro basin (Spain): Crops and irrigation systems

Irrigation performance assessments are required for hydrological planning and as a first step to improve water management. The objective of this work was to assess seasonal on-farm irrigation performance in the Ebro basin of Spain (0.8 million ha of irrigated land). The study was designed to address the differences between crops and irrigation systems using irrigation district data. Information was only available in districts located in large irrigation projects, accounting for 58% of the irrigated area in the basin. A total of 1617 records of plot water application (covering 10,475 ha) were obtained in the basin. Average net irrigation requirements (IR_n) ranged from 2683 m³ ha⁻¹ in regulated deficit irrigation (RDI) vineyards to 9517 m³ ha⁻¹ in rice. Average irrigation water application ranged from 1491 m³ ha⁻¹ in vineyards to 11,404 m³ ha⁻¹ in rice. The annual relative irrigation supply index (ARIS) showed an overall average of 1.08. Variability in ARIS was large, with an overall standard deviation of 0.40. Crop ARIS ranged between 0.46 and 1.30. Regarding irrigation systems, surface, solid-set sprinkler and drip irrigated plots presented average ARIS values of 1.41, 1.16 and 0.65, respectively. Technical and economic water productivities were determined for the main crops and irrigation systems in the Aragón region. Rice and sunflower showed the lowest productivities. Under the local technological and economic constraints, farmers use water cautiously and obtain reasonable (yet very variable) productivities.     

Antioxidant Activity and Phenylpropanoids of Phlomis lychnitis L.: A Traditional Herbal Tea

Phlomis lychnitis L. (Lamiaceae) is consumed as a traditional herbal tea in Spain. The antioxidant-protective effects as well as its phytoconstituents have never been established. The ability of the methanolic extract to protect cells from oxidative stress was evaluated in rat pheochromocytoma cells (PC12) using hydrogen peroxide as toxic agent. The viability of PC12 cells pre-treated with the methanolic extract of Phlomis lychnitis, determined by the MTT and LDH assays, was significantly improved at the highest dose (p?<?0.01). The antioxidant activity was confirmed evaluating the capacity of the plant to scavenge ABTS, DPPH, O₂ · ^? radicals and to inhibit XO. Bioassay guided fractionation led to antioxidant compounds. Qualitative HPLC/DAD/ESI/MS analysis reported phenylpropanoids, verbascoside being the major antioxidant constituent.     

Effect of winter cover crop species and planting methods on maize yield and N availability under irrigated Mediterranean conditions

Under semiarid Mediterranean conditions irrigated maize has been associated to diffuse nitrate pollution of surface and groundwater. Cover crops grown during winter combined with reduced N fertilization to maize could reduce N leaching risks while maintaining maize productivity. A field experiment was conducted testing two different cover crop planting methods (direct seeding versus seeding after conventional tillage operations) and four different cover crops species (barley, oilseed rape, winter rape, and common vetch), and a control (bare soil). The experiment started in November 2006 after a maize crop fertilized with 300 kg N ha⁻¹ and included two complete cover crop-maize rotations. Maize was fertilized with 300 kg N ha⁻¹ at the control treatment, and this amount was reduced to 250 kg N ha⁻¹ in maize after a cover crop. Direct seeding of the cover crops allowed earlier planting dates than seeding after conventional tillage, producing greater cover crop biomass and N uptake of all species in the first year. In the following year, direct seeding did not increase cover crop biomass due to a poorer plant establishment. Barley produced more biomass than the other species but its N concentration was much lower than in the other cover crops, resulting in higher C:N ratio (>26). Cover crops reduced the N leaching risks as soil N content in spring and at maize harvest was reduced compared to the control treatment. Maize yield was reduced by 4 Mg ha⁻¹ after barley in 2007 and by 1 Mg ha⁻¹ after barley and oilseed rape in 2008. The maize yield reduction was due to an N deficiency caused by insufficient N mineralization from the cover crops due to a high C:N ratio (barley) or low biomass N content (oilseed rape) and/or lack of synchronization with maize N uptake. Indirect chlorophyll measurements in maize leaves were useful to detect N deficiency in maize after cover crops. The use of vetch, winter rape and oilseed rape cover crops combined with a reduced N fertilization to maize was efficient for reducing N leaching risks while maintaining maize productivity. However, the reduction of maize yield after barley makes difficult its use as cover crop.