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Nadeau JH Balling R Barsh G Beier D Brown SD Bucan M Camper S Carlson G Copeland N Eppig J Fletcher C Frankel WN Ganten D Goldowitz D Goodnow C Guenet JL Hicks G Hrabe de Angelis M Jackson I Jacob HJ Jenkins N Johnson D Justice M Kay S Kingsley D Lehrach H Magnuson T Meisler M Poustka A Rinchik EM Rossant J Russell LB Schimenti J Shiroishi T Skarnes WC Soriano P Stanford W Takahashi JS Wurst W Zimmer A;International Mouse Mutagenesis Consortium 《Science (New York, N.Y.)》2001,291(5507):1251-1255
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Olga A. Kildisheva Todd E. Erickson David J. Merritt Matthew D. Madsen Kingsley W. Dixon Jacqueline Vargas Remy Amarteifio Andrea T. Kramer 《Strength and Conditioning Journal》2018,71(3):318-322
Seed dormancy can present a significant barrier to restoration outcomes in dryland systems. Physical and combinational (physical + physiological) dormancy are prevalent among seeds of many herbaceous perennials used in restoration of drylands throughout the western United States. Although many techniques designed to alleviate these dormancy traits exist, their efficacy is species specific, may result in embryo damage, and may have limited large-scale application. To identify the most effective means of dormancy alleviation with the potential to be used on an operational scale, we examined the effects of 16 temperature-based techniques (altering temperature and duration of wet heat, freezing and wet heat, and freeze-thaw cycles) and 6 abrasion-based techniques (altering pneumatic scarification length or using a single duration of manual scarification) on the enhancement of seed permeability among two physically dormant (western prairie clover [Dalea ornata {Douglas} Eaton & Wright] and Munro’s globemallow [Sphaeralcea munroana {Douglas} Spach]) and two combinationally dormant species (basalt milkvetch [Astragalus filipes Torr. ex A. Gray] and longspur lupine [Lupinus arbustus {Douglas} ex Lindl.]). We first assessed seed imbibition following exposure to all temperature- and abrasion-based techniques to identify those most successful at promoting seed permeability and then evaluated the effectiveness of those techniques through a series of germination experiments. For combinationally dormant species, we also tested whether exposure to GA3 enhanced germination. Abrasion-based techniques were more effective than temperature-based techniques at improving water uptake across all species. Pneumatic scarification significantly improved germination, but optimal treatment durations were species specific. GA3 did not enhance germination under the tested conditions. We conclude that pneumatic scarification is a fast, safe, and effective method for alleviating physical seed dormancy with a potential to be scaled up for operational use in restoration. 相似文献
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OBJECTIVES: To develop a composite index to describe the overall breast-feeding performance of infants<6 months of age; and, using this index, to identify the factors associated with poor breast-feeding practices and the association between breast-feeding and infant morbidity. DESIGN, SETTING AND SUBJECTS: The 2003 Demographic and Health Survey was a multi-stage cluster sample survey of 4320 households in Timor-Leste which covered 573 infants aged<6 months. Breast-feeding Performance Index (BPI) was constructed by allocating one point for each of seven infant feeding practices: first suckling within an hour of birth; absence of prelacteals; non-use of feeding bottles; current breast-feeding; not receiving liquids; not receiving formula or other milk; and not receiving solids in the last 24 hours. BPI was treated as the dependent variable in univariate and multivariate analyses to identify the factors associated with poor breast-feeding. RESULTS: Exclusive breast-feeding rate was 29.9%. The BPI (mean 4.4, standard deviation 1.77) was categorised as low, average and high according to tertiles. Multivariate analysis indicated that infants from the richest households were 1.70 (95% confidence interval (CI) 1.04-2.77) times more likely to have 'low BPI' than the poorest. Maternal BMI<18.5 kg m(-2) was predictive of poor breast-feeding (odds ratio=1.79; 95% CI 1.27-2.52). In the 'low' BPI group, the incidence of diarrhoea (13.4%) and acute respiratory infections (20.7%) during the previous two weeks was significantly higher than in 'average' (4.3 and 9.3%) and 'high' BPI groups (4.6 and 5.5%). CONCLUSIONS: Creating a composite index to assess the overall breast-feeding performance among infants<6 months of age is feasible. BPI can be effectively used to identify target groups for breast-feeding promotion interventions. 相似文献
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Flematti GR Goddard-Borger ED Merritt DJ Ghisalberti EL Dixon KW Trengove RD 《Journal of agricultural and food chemistry》2007,55(6):2189-2194
The butenolide, 3-methyl-2H-furo[2,3-c]pyran-2-one (1), has recently been identified as the germination stimulant present in smoke that promotes the germination of seeds from a wide range of plant species. In this paper, we describe the preparation of a number of analogues of 1 and compare their efficacy in promoting seed germination of three highly smoke-responsive plant species, Lactuca sativa L. cv. Grand Rapids (Asteraceae), Emmenanthe penduliflora Benth. (Hydrophyllaceae), and Solanum orbiculatum Poir. (Solanaceae). The results show that the methyl substituent at C-3 in 1 is important for germination-promoting activity while substitution at C-7 reduces activity. In contrast, bioactivity is mostly retained with analogues substituted at C-4 or C-5. 相似文献
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