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1.
Carmela Gallo Giusi Barra Marisa Saponaro Emiliano Manzo Laura Fioretto Marcello Ziaco Genoveffa Nuzzo Giuliana dIppolito Raffaele De Palma Angelo Fontana 《Marine drugs》2020,18(12)
Immunotherapy takes advantage of the immune system to prevent, control, and eliminate neoplastic cells. The research in the field has already led to major breakthroughs to treat cancer. In this work, we describe a platform that integrates in vitro bioassays to test the immune response and direct antitumor effects for the preclinical discovery of anticancer candidates. The platform relies on the use of dendritic cells that are professional antigen-presenting cells (APC) able to activate T cells and trigger a primary adaptive immune response. The experimental procedure is based on two phenotypic assays for the selection of chemical leads by both a panel of nine tumor cell lines and growth factor-dependent immature mouse dendritic cells (D1). The positive hits are then validated by a secondary test on human monocyte-derived dendritic cells (MoDCs). The aim of this approach is the selection of potential immunotherapeutic small molecules from natural extracts or chemical libraries. 相似文献
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Ohne ZusammenfassungDie Autoren sprechen Dr. Otto Schubart, Biologen der Estação Experimental de Biologia e Piscicultura, Pirassununga, Estado de São Paulo, ihren Dank aus für die Übersetzung dieses Artikels ins Deutsche, sowie Dr. Francisco A. Menezes Mariconi vom Instituto Biológico in São Paulo für verschiedene Ratschläge. 相似文献
4.
Mario J. Pastorino Soledad Ghirardi Javier Grosfeld Leonardo A. Gallo Javier G. Puntieri 《Annals of Forest Science》2010,67(5):508-508
5.
The isolation, (13)C-NMR and CD data of two 7-epimer oxindole alkaloids, 10,11-dimethoxyisomitraphylline (1) and 10,11-dimethoxymitraphylline (2) from Cabucala cryptophlebia are reported. 相似文献
6.
Javier Puntieri Javier Grosfeld Marina Stecconi Cecilia Brion María Marta Azpilicueta Leonardo Gallo Daniel Barthélémy 《Annals of Forest Science》2007,64(8):839-844
Shoot growth and dieback were compared among progenies of nursery-grown seedlings of Nothofagus obliqua belonging to seven progenies of the same provenance (Quila-Quina, Argentina). First-year shoots consisted of one growth unit (GU) and second-year shoots of one or two GUs. The probability of development of two GU was similar for all progenies. Progenies were different in terms of shoot size, terminal bud abscission, the extent of shoot dieback after shoot extension and the node of origin of the relay shoot on the first shoot. Plants with a second-year shoot consisting of two GUs had a thicker stem and more nodes than those with single-GU shoots. The selection of N. obliqua seed trees based on architectural traits suitable for forestry development at specific sites must contemplate variability among progenies and their probabilities of successful development under different conditions. 相似文献
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Patterns of shoot elongation of 2-yr seedlings from native North American populations of ponderosa pine and Douglas-fir were compared to those of Argentine land races originating from unknown provenances. The comparisons were conducted in Moscow, Idaho (USA), and suggested that the ponderosa pine land race was descended from a California provenance at low or middle elevations but that the growth potential of the land race was only mediocre in comparison to eight native populations. The Douglas-fir land race exhibited a relatively high growth potential in comparison to 19 native provenances and undoubtedly originated from a mild coastal environment. The results provide concrete recommendations for upgrading the growth potential of the Argentine land races by importing germ plasm of specific provenances. 相似文献
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2n Gamete formation in the genus Brachiaria (Poaceae: Paniceae) 总被引:1,自引:0,他引:1
Patrícia Helena Gallo Pamela Lonardoni Micheletti Kellen Regina Boldrini Claudicéia Risso-Pascotto Maria Suely Pagliarini Cacilda Borges do Valle 《Euphytica》2007,154(1-2):255-260
Microsporogenesis of several Brachiaria species of the Brazilian collection at Embrapa Beef Cattle has been analyzed in detail. This paper reports abnormal cytokinesis
in three accessions of three different species (Brachiaria humidicola, 2n = 4x = 36, Brachiaria decumbens, 2n = 4x = 36, and Brachiaria dura, 2n = 6x = 54). Chromosomes paired in bi-, tri-, and quadrivalents in these accessions, whereas chromosome segregation at meiosis
I was characterized by exclusion of laggards as micronuclei. In a high number of meiocytes, the first sign of cytokinesis
appeared only in metaphase II and did not divide the meiocyte into a dyad. Total absence of cytokinesis was also detected
among meiocytes in the second division. Since in both cases the two metaphase plates were very close, they favored the rejoining
of chromosome sets after anaphase II and formed a restitutional nucleus in telophase II. Second cytokinesis occurred after
telophase II in most meiocytes. Monads, dyads, and triads with n or 2n nuclei were observed among meiotic products. The 2n gametes observed correspond to the first division restitution (FDR). The number of affected cells in each accession was variable,
but the number of microspores with restitutional nucleus, including those scored in tetrads and the released ones, did not
exceed 9%. Although polyploidy is common in the genus Brachiaria, its origin is still unclear. Current results suggest that 2n gametes may have contributed to the evolutionary history of the genus. 相似文献
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