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利用自育的核三系艋保系新材料绵7MB-1,可以将油菜绵7AB类型、S45AB类型等臆性核不育两用系的不育株率从50%提高到90%以上。针对该材料在自交繁殖过程中,发生部分育性回复的现象,采用临保系植株茎段体细胞组织培养技术,实现了绵7MB-1的批量繁殖,将繁殖的临保系苗用于网室亲本不育系繁制,可使不育系亲本不育株率达到91.7%-93.5%。每繁制公顷不育系亲本,可供7500—15000hm^2杂交F1的制种生产使用。用高不育率不育系代替原核不育两系进行制种,大大减少了用工,同时使制种产量显著提高,制种质量也更有保证,形成了完善的繁殖与制种体系。  相似文献   
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绵油11号直播与育苗移栽的不同施肥水平与不同密度的对比试验结果表明,直播油菜可以获得与常规育苗移栽相近的产量,适宜的种植密度为1.8~2.2万株/667m^2;与育苗移栽相比,直播油菜的生育期缩短,个体生长量偏小。单株角果数较少,但单位面积总角果数与育苗移栽相近。角粒数与千粒重变化不大;直播油菜能降低成本,节省用工,效益高。  相似文献   
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1材料与方法 1.1材料 核三系临保系:绵7MB-1;核两系不育系:绵7AB-4—1;恢复系:绵恢9613及品93-496(引自四川省农业科学院)。诱导培养基:B5+谷氨酰胺2mg/L+水解乳蛋白50mg/L+6-BA2mg/L+2T 1mg/L。增殖培养基:MS+6-BA 1 mg/L+NAA 0.1mg/L。  相似文献   
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[Objective] The aim was to study the reproduction of the three-line genic male sterile (GMS) line parent Mian 7MB-1 (B.Napus L.) and the seed production of F1 through somatic tissue culture. [Method] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7%-93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500-15 000 hm2. [Conclusion] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.  相似文献   
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