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[Objective] This work was aimed to explore the mechanism of Hg2+ toxicity on plants.[Method]Activities of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)were investigated in wheat(Triticum aestivum L.)seedlings under Hg2+ stress at different concentrations.[Result]① There were no obvious effects on the growth of seedlings when the concentration of Hg2+ was lower than 0.10 mmol/L.However,toxic effects on the growth of seedling were observed when the concentration of Hg2+ was higher than 0.10 mmol/L.② Different tissues showed different resistant ability in response to Hg2+ stress.The leaves and roots of wheat seedlings were more insensitive to Hg2+ toxicity.③ CAT was more sensitive to Hg2+ stress compared to POD and SOD.[Conclusion]The toxic effect was related to the concentration of Hg2+(0.10 mmol/L).The higher concentration of Hg2+ could affect the expression of POD,CAT,and SOD isozymes in the leaves,roots of wheat seedlings and germinated seeds,which further affect the normal metabolism of membrane lipid and inhibit the growth of wheat seedlings at last.  相似文献   
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Hg2+胁迫对小麦幼苗POD、CAT和SOD同工酶的影响   总被引:12,自引:0,他引:12  
[Objective] This work was aimed to explore the mechanism of Hg2+ toxicity on plants. [Method]Activities of peroxidase(POD), catalase (CAT) and superoxide dismutase(SOD) were investigated in wheat (Triticum aestivum L. )seedlings under Hg2+ stress at different concentrations. [Re-pared to POD and SOD. [Conclusion] The toxic effect was related to the concentration of Hg2+ (0.10 mmol/L). The higher concentration of Hg2+ could affect the expression of POD, CAT, and SOD isozymes in the leaves, roots of wheat seedlings and germinated seeds, which further affect the normal metab-olism of membrane lipid and inhibit the growth of wheat seedlings at last.  相似文献   
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[目的]为研究Hg2+对植物的毒害机理提供理论依据。[方法]以小麦为材料,研究了不同浓度Hg2+胁迫对小麦幼苗中的过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)同工酶活性变化的影响。[结果]在Hg2+浓度低于0.100 mmol/L时,对小麦幼苗生长的影响不明显;在Hg2+浓度高于0.100 mmol/L时,将会显著阻碍小麦的正常生长发育。小麦幼苗的不同组织对Hg2+胁迫的抵抗强度有所不同,表现为其叶片和根的抗胁迫性比萌发的种胚要强。在小麦幼苗受Hg2+胁迫时,POD、CAT和SOD 3种抗氧化酶同工酶中,CAT同工酶最为敏感。[结论]0.100 mmol/LHg2+浓度是小麦幼苗能否正常生长的阈值。  相似文献   
4.
萝卜过氧化物酶基因片段的cDNA克隆及花期表达谱分析   总被引:1,自引:0,他引:1  
通过研究过氧化物酶(POD)活性在心里美萝卜花期的变化以及萝卜POD基因的结构,探讨POD的基因结构和功能之间的关系。测定了萝卜花期 不同时间各个部位中POD活性,用改进的半定量RT-PCR分析了过氧化物酶基因(Rsprx)的表达谱,并通过RT-PCR克隆过氧化物酶基因的cDNA片段。结 果表明,心里美萝卜花期不同器官中的POD活性具有明显的变化,地上部分和地下部分POD活性差异显著,在结荚期的木质部II中达到峰值(17.53± 0.35)×103 U•g-1(FW)。不同器官中Rsprx的表达差异明显,花蕾和荚中表达最强。从花蕾和荚中得到4个长度为400 bp左右的cDNA克隆片段,序列 比对结果显示,与棉花的花器官过氧化物酶基因Ghpod具有较高的同源性和结构相似性。因此认为POD与萝卜花期生长发育有一定的关系,且有多种 过氧化物酶基因在该过程中表达。  相似文献   
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以"心里美"萝卜为材料,研究了晚播导致亲代营养生长期经低温短光照胁迫对子代种子萌发以及苗期抗氧化酶活性等的影响.结果表明,处理组萝卜种子发芽指数以及幼苗活力指数、鲜重和根长与同期对照组(CK2)相比没有显著性差异,处理组苗高显著高于亲代对照组(CK1和CK2);处理组子叶的POD活性和根部可溶性蛋白含量显著高于CK2;根和下胚轴的α-淀粉酶活性显著高于CK2;POD和α-淀粉酶同工酶谱带在处理组、CK1和CK2同一器官间差异不明显.由此推论,亲代营养生长期经低温短光照胁迫对萝卜子代种子活力以及苗期生长没有显著的远期影响.  相似文献   
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