Die Schneebeere als neue Wirtspflanze der Kirschfruchtfliege

Ohne Zusammenfassung     

Detection of Xanthomonas fragariae in symptomless strawberry plants by nested PCR*

Xanthomonas fragariae spreads in symptomlessly infected strawberry plantlets and a method for detection of latent infections is necessary. It is a very slow‐growing bacterium in culture and is easily overgrown by saprophytic bacteria. Therefore, plating is not a suitable method for detecting low numbers of bacteria in symptomless plants. In addition, selective media are not available. Serological assays like immunofluorescence are useful for testing in‐vitro plants, but they are not suitable for field‐grown plants, as cross reactions are common with the available antisera. For these plants, nested PCR with primers from Pooler ( Pooler et al., 1996 ) and Zimmermann ( Zimmermann et al., 2004 ) has proved to be a valuable method. The method was successfully applied for a survey of strawberry plants from fields in Germany and for testing imported plants (frigo and green plants).     

Der Einsatz von Trichogramma-Schlupfwespen in Deutschland

The use of Trichogramma egg parasitoids has further increased. T. brassicae, released against the European Corn Borer Ostrinia nubilalis, holds the largest share in Germany with 11,000 hectare. For small-scale fruits crops the commercial use of T. cacoeciae and T. dendrolimi against the Codling Moth (Cydia pomonella) and the Plum Moth (Grapholita funebrana) has been expanded. Trichogramma species are also being offered for controlling the vine moths, Lobesia botrana and Eupoecilia ambiguella on grapevine in homegardens. A strain of T. evanescens is available against cabbage pests, especially noctuids. Research has been conducted on the biological control of Autographa gamma in spinach, as well as of the Pea Moth (Cydia nigricana) and the Leek Moth (Acrolepiopsis assectella) in organic farming. Trichogramma is also important for the biocontrol of exotic pests in greenhouses. These are often polyphagous pests that have been introduced together with ornamental plants, such as Duponchelia fovealis and Clepsis spectrana. The Carnation Tortrix (Cacoecimorpha pronuba) was successfully controlled by Trichogramma in the field. The use of Trichogramma has been further established for the control of storage pests. T. evanescens is used against the Indian Meal moth (Plodia interpunctella) in stored cereal products. Other target pests in storage environments are Ephestia kuehniella and Sitotroga cerealella. In addition, Trichogramma species have been tested for the control of the Common Cloths Moth (Tineola bisselliella) under laboratory conditions. The range of target pests for Trichogramma egg parasitoids has therefore increased considerably in research and commercial applications in Germany.     

Mycotoxicosis produced in swine by cultural products of an isolate of Aspergillus ochraceus. II. Clinicopathologic changes

Rice culture of a toxigenic strain of Aspergillus ochraceus was fed at a concentration of 25% to weanling pigs for 10 days. The clinicopathological abnormalities reflected renal damage. Activities of lactic dehydrogenase, glutamic-oxaloacetic transaminase and isocitrate dehydrogenase were increased in the urine but not in the serum. Serum concentrations of urea nitrogen and creatinine were high. Cellular and granular casts, blood, protein, and glucose were in the urine of pigs fed toxic diet. Serum concentrations of K+, Na+ and Cl- were unchanged, but concentrations of these electrolytes were reduced in the urine.     

Incidence of reinfections with Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in pig farms located in respiratory-disease-free regions of Switzerland--identification and quantification of risk factors

The objective of the study was to identify risk factors for reintroduction of Actinobacillus pleuopneumoniae and Mycoplasma hyopneumoniae (enzootic pneumonia) onto pig farms in areas in Switzerland that were involved in an eradication programme from 1996 to 1999 and to assess the role of dealers in relation to these reinfections. The study was based on the comparison of pig farms that were reinfected in the year 2000 (cases) and pig farms that remained uninfected in the same area (controls). Additionally, data were collected from Swiss pig dealers and transport companies. Out of a total of 3983 farms, 107 farms were reinfected in the year 2000. The incidences were 0.1% for Actinobacillus pleuopneumoniae and 2.6% for Mycoplasma hyopneumoniae (enzootic pneumonia). Compared to reinfection rates prior to the eradication programme, this is a considerable reduction. Statistically significant risk factors for the reinfection were 'finishing farm', 'large mixed breeding-finishing farm', 'reinfected neighbour' and 'parking site for pig transport vehicles close to the farm'. Pig farmers that purchased pigs from only one supplier per batch had a lower risk of reintroducing infection (protective factor). As long as infected and uninfected regions co-exist in Switzerland, direct and indirect contact between farms, pig herds and slaughter sites via transport vehicles are a major pathway of disease spread. Risk management measures linked to these contacts are therefore of key importance. The survey of dealers indicated various areas for improvement such as strategic planning of pick-up routes or cleaning and disinfecting of trucks.     

Persistent activity of doramectin and ivermectin in the prevention of cutaneous myiasis in cattle experimentally infested with Cochliomyia hominivorax

A study was conducted to evaluate the activity of a single administration of doramectin or ivermectin against severe, induced infestations of Cochliomyia hominivorax. Twenty-four Holstein bull calves were allocated to four groups of six animals each and treated either with saline, doramectin 1%, or either one of two formulations of ivermectin 1% at a dose rate of 200 microg/kg. On Day 12 after treatment, each calf was anesthetized and two wounds were created on the left side of the shoulder and rump of each calf and 2 h later, each wound was implanted with 100 newly hatched larvae of C. hominivorax. On Day 15 after treatment, the procedure was repeated on the right side of each calf. Wounds were examined daily for 5 days and evidence of live larvae was recorded. Doramectin provided reduction in myiasis of 90.9 and 83.3% at 12 and 15 days after treatment, respectively, compared to the saline control treatment (P < 0.0001). In contrast, there were no significant differences in the number of calves with myiasis between those treated with either of the ivermectin formulations and the saline control.