Soluble matrix of hen's eggshell extracts changes in vitro the rate of calcium carbonate precipitation and crystal morphology

1. Extra and intramineral eggshell matrix proteins were solubilised before and after demineralisation by sequential extractions using guanidine hydrochloride and EDTA.

2. The intramineral electrophoretic profile of SDS‐PAGE showed the presence of 80, 66, 43, 36 and 15 kDa bands with a predominance of a 17 kDa band. In the extramineral part, the major protein was the 15 kDa band.

3. The introduction of intramineral extract to a metastable solution of calcium carbonate delayed the rate of crystal growth. The delay in the rate of precipitation was elicited by a single fraction (MW 50–80 kDa), isolated by gel filtration chromatography, of eggshell extracts. Extramineral extracts had no effect.

4. Addition in vitro of intramineral eggshell extracts modified the morphology of calcite; the crystals aggregated and showed irregular surfaces.

5. These observations suggest that constituents of the eggshell matrix are involved in the control of calcite growth and crytallographic structure of the hen's eggshell.     

Myocarditis caused by naturally acquired canine distemper virus infection in 4 dogs

Canine distemper virus (CDV) has long been recognized as a cause of myocarditis; however, cases of myocarditis caused by naturally acquired CDV infection have been reported only rarely in dogs. We describe here our retrospective study of naturally acquired systemic CDV infection in 4 dogs, 4–7 wk old, that had myocarditis, with myocardial necrosis and fibrosis. One of the 4 dogs had intracytoplasmic eosinophilic inclusion bodies in cardiomyocytes. Other lesions included bronchointerstitial pneumonia (4 of 4), necrotizing hepatitis (2 of 4), splenic lymphoid necrosis (2 of 4), encephalitis (1 of 3; brain was not submitted in 1 case), and necrotizing gastroenteritis (1 of 4). The presence of CDV in the heart was confirmed by immunohistochemistry in all 4 dogs.     

Exploitation of predators in UK fruit and hop culture

A wide range of species of predators has been exploited, or has the potential to be exploited, as biological control agents against pests of fruit and hop. They include members of the families Miridae, Anthocoridae and Chrysopidae, and of the mite family Phytoseiidae. Some species can colonise fruit plantations naturally, whereas others are mass-cultured and introduced artificially. The characteristics of the various predator species are considered in relation to the requirements for biological control agents in specific crop situations.     

Transmission electron microscopy of the vertical crystal layer and cuticle of the eggshell of the domestic fowl

1. Eggshell pieces (1 cm2) were decalcified using a solution of EDTA (200 g/litre, pH 6.9 to 7.0) in paraformaldehyde (2 g/litre) and glutaraldehyde (0.5 g/litre) in phosphate buffer. 2. They were prepared for transmission electron microscopy (TEM). 3. TEM identified a vertically aligned matrix associated with the vertical crystal layer (VCL). It is hypothesised that the vertical orientation of calcite crystals in the VCL is closely linked to this vertical matrix. 4. TEM also revealed the presence of a 2-layered cuticle, the inner layer containing vesicles which were absent in the outer. 5. Cuticular vesicles contain hydroxyapatite and are thought to play a role in the termination of shell formation. The current paper presents data relating to microbial apatitic systems that strengthen this hypothesis.     

The use of frequency diagrams in the survey of resistance to pesticides in ticks in Southern Africa

A method whereby resistance data can be analysed by means of frequency distributions is described. This method established that Rhipicephalus appendiculatus and R. evertsi evertsi show either little or only developing resistance to the pesticides chlorfenvinphos and dioxathion. Boophilus spp., however, show more instances and higher levels of resistance to these 2 pesticides.     

Structural Characterisation of the Interaction between Triticum aestivum and the Dothideomycete Pathogen Stagonospora nodorum

The interaction between Stagonospora nodorum and a susceptible wheat cultivar was investigated using a range of microscopic techniques. Germination of pycnidiospores occurred approximately 3 h after making contact with the leaf surface and was followed by attempted penetration 8–12 h later. Penetration was observed through stomata and also directly through periclinal and anticlinal epidermal cell walls. Penetration down the anticlinal cell walls appeared to occur without a differentiated penetrating structure whilst structures identified as either lateral appressoria or hyphopodia were typically present when penetrating over a periclinal cell wall. Once inside the leaf, the fungus continued to grow for the next 4–5 days colonising all parts of the leaf except the vascular bundles. Only in the later phase of the infection was total host cell collapse apparent. Evidence of polyphenolic compounds was observed. The infection cycle was completed within 7 days as indicated by sporulation on the leaf surface. These results have allowed us to understand how the fungus physically interacts with the leaf and will help the overall understanding of the infection process.     

Livestock water productivity in mixed crop–livestock farming systems of the Blue Nile basin: Assessing variability and prospects for improvement

Water scarcity is a major factor limiting food production. Improving Livestock Water Productivity (LWP) is one of the approaches to address those problems. LWP is defined as the ratio of livestock’s beneficial outputs and services to water depleted in their production. Increasing LWP can help achieve more production per unit of water depleted. In this study we assess the spatial variability of LWP in three farming systems (rice-based, millet-based and barley-based) of the Gumera watershed in the highlands of the Blue Nile basin, Ethiopia. We collected data on land use, livestock management and climatic variables using focused group discussions, field observation and secondary data. We estimated the water depleted by evapotranspiration (ET) and beneficial animal products and services and then calculated LWP. Our results suggest that LWP is comparable with crop water productivity at watershed scales. Variability of LWP across farming systems of the Gumera watershed was apparent and this can be explained by farmers’ livelihood strategies and prevailing biophysical conditions. In view of the results there are opportunities to improve LWP: improved feed sourcing, enhancing livestock productivity and multiple livestock use strategies can help make animal production more water productive. Attempts to improve agricultural water productivity, at system scale, must recognize differences among systems and optimize resources use by system components.     

Water circulation, groundwater outflow and nutrient dynamics in Mida creek, Kenya

The relationship between physical hydrodynamic processes and nutrients dynamics was investigated in Mida creek, a groundwater influenced mangrovefringed creek in Kenya between March 1996 and May 1997. The research involved spot and timeseries measurement of nitrate–nitrite, ammonia, silicates, phosphates, salinity, temperature, sealevel as well as tidal currents at seven stations located in the front, middle and backwater zones of the creek. Groundwater level as well as total dissolved solids' concentration, salinity, temperature and nutrients' concentration were also measured once every month in shallow wells (watertable<5m) located in the upper region of the creek. Results of the study show that nutrient concentrations vary with the tide and that, though there is no river drainage, they are of the same magnitude as in mangrove creeks with substantial river runoff. The peak concentrations of NH ₄ ⁺ –N (5.45M), NO ₂ –NO ₃ (5.63M), PO ₄ ^3– –P (0.58M) and SiO ₃ ^2– –Si (81.36M) in the creek occurred during flood tide, 2–3h before high waters. The (NO ₂ ^– + NO ₃ ^– )–N concentrations declined rapidly during ebb tide, reaching the minimum levels during low water. Contribution of groundwater seepage to the net nutrients flux (particularly on nitrite–nitrates) is largest in dry seasons. The study shows that groundwater outflow sustains the mangroves during periods of severe salinity stress and nutrients deficiency in dry seasons. This is essentially by limiting salinity increase and by boosting nutrient supply in dry seasons.     

Antimicrobial flavonoids from the stem bark of Erythrina burttii

The chloroform extract of the stem bark of Erythrina burttii showed antifungal and antibacterial activities using the disk diffusion method. Flavonoids were identified as the active principles. Activities were observed against fungi and Gram(+) bacteria, but the Gram(-) bacteria Escherichia coli was resistant.     

Long-term electricity production from soil electrogenic bacteria and high-content screening of biofilm formation on the electrodes

Purpose

This study was conducted to determine the existence of soil bacteria in soil by soil microbial fuel cell (SMFC). The main objectives were (1) to differentiate the type of soil which will influence the electricity production, (2) to demonstrate the impact of different volume of soil in the MFC and feeding MFC for long-term electricity production, and (3) to conclude that electricity production is directly proportional to the biofilm formation on the anode surface.

Materials and methods

MudWatt kits were purchased from Keego Technologies USA, and 22 identical SMFCs were designed to study the electricity production from agricultural soil (S1) and vermicompost soil (S2). Ten milliliters of bioslurry is fed in SMFC to study the stability of electricity production at different stages. Microbes were isolated and characterized from the surface of the electrode. Biofilm analyses were done by high-content screening (HCS) system using 10 μl of acridine orange (100 μg/ml) at different stages of biofilm, and scanning electron microscopy is applied to confirm the matured biofilm on the surface of the anode.

Results and discussion

Application of bioslurry at different stages of electricity production conquers the normal energy recovery of the SMFCs and S2 soil with bioslurry sample produced the highest open circuit voltage (OCV) of 2.8 V (460 days) and S1 soil sample with bioslurry produced 1.7 V (364 days). The difference between SMFCs and MudWatt kits significantly confirms that increasing the volume of soil in the anode part increases the electricity production. The maximum OCV of S1 and S2 in MudWatt kits were 1.5 V (90 days) and 1.8 V (190 days), respectively. Increased volume of soil in our SMFCs produce maximum OCV of 1.8 V (S1 for 173 days) and 2.2 V (S2 for 240 days), and HCS analysis of biofilm at different stages reveals that electricity production is directly proportional to the biofilm formation.

Conclusions

Thus, it was concluded that the nature of soil and soil bacterium is important for the electricity production, and S2 soil sample produces maximum electricity than the S1 soil sample. Feeding of SMFCs with bioslurry aids the long-term and stabilized electricity production in both the soil samples.