Single oral β-cryptoxanthin administration increases its serum concentration and enhances peripheral blood neutrophil function in Holstein cattle

We investigated the effect of oral administration of β-cryptoxanthin (β-CRX) on its serum concentration and peripheral neutrophil functions by the chemiluminescence (CL) response in Holstein cattle. A single oral administration of β-CRX was performed for serum β-CRX concentration (0, 0.05, 0.1, or 0.2 mg/kg body weight [BW]) and for peak CL response of peripheral neutrophils (0.2 mg/kg BW). The serum β-CRX concentration was peaked on 2 days after, similar to peak CL response on 3 days after β-CRX administration. Therefore, a single oral administration of β-CRX (0.2 mg/kg BW) induces higher serum concentration and concurrently enhances bactericidal ability of peripheral neutrophils in Holstein cattle.     

Suppression of MyoD induces spontaneous adipogenesis in skeletal muscle progenitor cell culture

The degree of intramuscular adipose tissue accumulation is one of the factors affecting meat quality. Accumulation of adipocytes is also observed under the pathological condition of skeletal muscle such as muscular dystrophy and sarcopenia. The origin of adipocytes seen in skeletal muscle is mesenchymal progenitor cells that can give rise to both adipocytes and fibroblasts. In the present study, we demonstrated that siRNA-mediated suppression of MyoD expression in rat skeletal muscle progenitor cell culture, which comprises both myogenic satellite cells and mesenchymal progenitor cells, resulted in diminished myotube formation and an unexpected spontaneous appearance of white adipocytes. Suppressing myomaker expression also resulted in complete absence of myotube formation without reducing MyoD expression, but no adipogenesis was seen in this scenario, indicating that decline in MyoD expression rather than decreased myotube formation is necessary to induce adipogenesis. In addition, spontaneous adipogenesis induced by suppressing MyoD expression in culture was inhibited by the conditioned medium from control culture, indicating that anti-adipogenic factor(s) are secreted from MyoD-positive myogenic cells. These results indicate the presence of regulatory mechanism on adipogenesis by myogenic cells.     

Stocking effectiveness of hatchery-released kuruma prawns estimated by two-stage sampling of commercial catch in Ariake Sound, Japan

ABSTRACT:    To evaluate the stocking effectiveness of the kuruma prawn Penaeus japonicus , a two-stage sampling survey of Gensiki bottom-drift-netters was carried out in Ariake Sound, Saga Prefecture, Japan. A total of 471 000 hatchery-produced juveniles, marked by cutting off the right uropods, was released. Thirty-three fishing days were randomly drawn from four boats, which were selected from the 80 boats operating in the fishery. All prawns caught on the selected days were examined, and the hatchery-produced individuals were identified by their regenerated uropods. A telephone survey of all boats was also conducted, to determine the number of fishing days. A total of 286 marked prawns were caught on the survey days for the Saga area in Ariake Sound, from the last half of July to the first half of September 2002. The recapture rate and standard error were estimated at 0.76 ± 0.52%. Economic efficiency was estimated at 0.28 ± 0.02. Strategies for sampling schemes and stock recovery are also discussed.     

Influence of overplanting paddy rice on irrigation water delivery performance: a case study in the Dakalt branch canal,Nile Delta of Egypt

In the North Nile Delta of Egypt, the impacts of overplanting paddy rice on water delivery performance have not been discussed quantitatively. Further, the amount of water that could have been saved if farmers would follow the planned area is unknown. In this study, water delivery performance was assessed by comparison of actual paddy rice planting and the government’s planned conditions. For both conditions, performance indicators relating to adequacy, equity, and dependability were analyzed across six locations in conjunction with the branch canal water level in 2013 and 2014. Based on the difference between the actual water supply and planned water demand, the amount of water that could have been saved for downstream uses was calculated. The average adequacy for the investigation period was good at one location, fair at 2 locations, and poor at 3 locations in both years. Further, adequacy under both actual and planned conditions was poor in late July at all locations. The planned adequacy and dependability downstream and equity among locations improved compared to the actual condition in both years. Under the condition that paddy rice area is the upper limit planned by the government, about 12.3% and 9.6% of water could be potentially saved in each year. The difference between actual and planned water delivery performance is caused by the branch canal’s low water level. Control of overplanting paddy rice and coordination of water distribution among water user associations would improve stable water level in the canal and, eventually, water delivery performance.

     

Radiocarbon Content in Urban Atmospheric Aerosols

We attempted to elucidate seasonal variations in fossil-fuel-derived carbon (FC) and biomass-derived carbon (BC) in urban atmospheric aerosols. We undertook continuous measurements of the composition of fine particle (PM_2.1) in central Tokyo, including the ¹⁴C/¹²C ratio. The percent modern carbon (pMC) contained in all samples averaged 43, and the highest was 54 in late December and the lowest was 31 in early August. From the observed carbonaceous component concentrations and the pMC we could calculate the content ratio of FC and BC in PM_2.1 and investigate their seasonal variations. Although there was almost no seasonal variation in the ratio of FC, the ratio of BC was observed to rise in early winter. This indicates that FC is influenced by the emission sources without seasonal variations (such as automobiles driven in urban areas). Furthermore, there is significant correlation between BC and organic carbon (OC), and even for urban areas, it is considered that the contribution of biomass carbon to OC in PM_2.1 is high.     

Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice

Apoptotic cells expose phosphatidylserine and are swiftly engulfed by macrophages. Milk fat globule epidermal growth factor (EGF) factor 8 (MFG-E8) is a protein that binds to apoptotic cells by recognizing phosphatidylserine and that enhances the engulfment of apoptotic cells by macrophages. We report that tingible body macrophages in the germinal centers of the spleen and lymph nodes strongly express MFG-E8. Many apoptotic lymphocytes were found on the MFG-E8-/- tingible body macrophages, but they were not efficiently engulfed. The MFG-E8-/- mice developed splenomegaly, with the formation of numerous germinal centers, and suffered from glomerulonephritis as a result of autoantibody production. These data demonstrate that MFG-E8 has a critical role in removing apoptotic B cells in the germinal centers and that its failure can lead to autoimmune diseases.     

Analysis of hop-derived terpenoids in beer and evaluation of their behavior using the stir bar-sorptive extraction method with GC-MS

Hop aroma components, which mainly comprise terpenoids, contribute to the character of beers. However, pretreatments are necessary before analyzing these components because of their trace levels and complicated matrixes. Here, the stir bar-sorptive extraction (SBSE) method was used to detect and quantify many terpenoids simultaneously from small samples. This simple technique showed low coefficients of variation, high accuracy, and low detection limits. An investigation of the behavior of terpenoids identified two distinct patterns of decreasing concentration during wort boiling. The first, which was seen in myrcene and linalool, involved a rapid decrease that was best fitted by a quadratic curve. The second, which was observed in beta-eudesmol, humulene, humulene epoxide I, beta-farnesene, caryophyllene, and geraniol, involved a gentle linear decrease. Conversely, the concentration of beta-damascenone increased after boiling. As the aroma composition depended on the hop variety, we also examined the relationship between terpenoid content and sensory analysis in beer.     

Effects of late sowing on soybean yields and yield components in southwestern Japan

ABSTRACT

Soybean production in southwestern Japan tends to be unstable owing to wet soils during the rainy season. Although late sowing after the rainy season can avoid excess water, information on its yield potential is limited. The objective of this study was to reveal the effect of late sowing on yields and yield components of new soybean cultivars developed for warm regions. The experiment was conducted in 2016 and 2017 in Fukuyama, Hiroshima, Japan. Upland fields converted from paddy fields with a subirrigation system were planted in June (normal) or July (late sparse or late dense). Lodging was prevented with a net. The effects of late sowing and dense treatment were analyzed in relation to solar radiation use. In 2016, differences in yield among cultivars and among environments were not significant. In 2017, yield was significantly reduced following late sparse sowing. The total aboveground dry matter at maturity was correlated with total solar radiation intercepted (r = 0.76) but not with radiation use efficiency (r = 0.47). Late sowing increased harvest index (HI) significantly from 0.464 to 0.571 in 2016 and from 0.524 to 0.585 in 2017, but density had no significant effect. The changes in HI were correlated with stem dry weight (r = ?0.80 in 2016 and r = ?0.79 in 2017) rather than seed yield (r = 0.08, n.s. in 2016 and r = 0.19, n.s. in 2017). Thus, under irrigation, late dense sowing might stabilize yield in southwestern Japan because of higher HI.

Abbreviations: DM: dry matter; FOEAS: farm-oriented enhancing aquatic system; HI: harvest index; RUE: radiation use efficiency     

Effects of maturity genes E2 and E3 on yield formation in soybean cultivar Enrei in warm region,Fukuyama in Japan

ABSTRACT

Understanding how maturity genes affect soybean yield formation will provide important information for crop management decisions. This study aimed to reveal how maturity genes E2 and E3 in the soybean cultivar ‘Enrei’ affect yields and yield formation in warm regions of Japan. ‘Enrei’ (e2e3) and three near-isogenic lines of ‘Enrei’ (e2E3, E2e3, and E2E3) were cultivated in 2016 and 2017 in Fukuyama, Japan (34°30′N, 133°23′E). Two sowing dates were set in each year (June sowing and July sowing). E2 extended the period from emergence to R1 and also the period from R1 to R7, whereas E3 extended only the period from emergence to R1. Interaction between E2 and E3 did not affect duration of the period from emergence to R1, but did affect the period from R1 to R7. Although seed yield did not differ between genotypes in the June sowings, the effects of E2 and E3 on seed yield in July sowing were both significant and interaction between E2 and E3 also observed. The total number of nodes increased in E3 genotypes in both sowing dates, especially in E2E3. Pod-set ratio was lower in E2 and E3 genotypes than in e2 and e3 genotypes in the June sowings, but did not differ between genotypes in the July sowings. The high yield of E2E3 genotypes in the July sowings was attributed to increased number of nodes and flower production while maintaining pod-set ratio. Appropriate choice of sowing date is suggested to be essential when using E3 genotypes.Abbreviations: HI: harvest index; NIL: near-isogenic line; RUE: radiation use efficiency; TDM: total above-ground dry matter; TRI: total solar radiation intercepted