全文获取类型
收费全文 | 1443篇 |
免费 | 124篇 |
国内免费 | 5篇 |
专业分类
林业 | 103篇 |
农学 | 45篇 |
基础科学 | 16篇 |
297篇 | |
综合类 | 203篇 |
农作物 | 72篇 |
水产渔业 | 140篇 |
畜牧兽医 | 520篇 |
园艺 | 35篇 |
植物保护 | 141篇 |
出版年
2023年 | 9篇 |
2022年 | 11篇 |
2021年 | 17篇 |
2020年 | 28篇 |
2019年 | 61篇 |
2018年 | 31篇 |
2017年 | 39篇 |
2016年 | 39篇 |
2015年 | 37篇 |
2014年 | 39篇 |
2013年 | 75篇 |
2012年 | 86篇 |
2011年 | 94篇 |
2010年 | 67篇 |
2009年 | 63篇 |
2008年 | 88篇 |
2007年 | 73篇 |
2006年 | 76篇 |
2005年 | 63篇 |
2004年 | 72篇 |
2003年 | 79篇 |
2002年 | 80篇 |
2001年 | 22篇 |
2000年 | 18篇 |
1999年 | 18篇 |
1998年 | 10篇 |
1997年 | 19篇 |
1996年 | 9篇 |
1995年 | 7篇 |
1994年 | 9篇 |
1993年 | 8篇 |
1992年 | 6篇 |
1991年 | 12篇 |
1990年 | 11篇 |
1989年 | 18篇 |
1987年 | 9篇 |
1986年 | 13篇 |
1985年 | 9篇 |
1984年 | 11篇 |
1983年 | 11篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 6篇 |
1978年 | 7篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1974年 | 9篇 |
1973年 | 6篇 |
1972年 | 5篇 |
1966年 | 5篇 |
排序方式: 共有1572条查询结果,搜索用时 31 毫秒
1.
Wardatou Boukari Denis Filloux Jean Heinrich Daugrois Emmanuel Fernandez Dimitre Mollov Claudia Kaye Martha Hincapie Alexander Sanchez Jianping Wang Philippe Roumagnac Philippe Rott 《Plant pathology》2020,69(6):1060-1069
Viruses in the genus Mastrevirus (family Geminiviridae), including those infecting sugarcane, have natural geographical ranges almost exclusively restricted to Africa and the Indian Ocean islands off the African coast. Only sugarcane white streak virus (SWSV) in Barbados and sugarcane striate virus (SStrV) in Florida and Guadeloupe are known to infect a few sugarcane varieties in the Western Hemisphere. In this study, PCR assays were developed to detect these two viruses in sugarcane. Five hundred and seventy-one DNA samples from Saccharum species and interspecific hybrids from the Miami World Collection of sugarcane and related grasses were tested for the presence of SStrV and SWSV by PCR. No variety was found infected by SWSV but SStrV was detected in 19 varieties. PCR data were confirmed by sequencing amplified fragments (248 bp). These fragments shared 93%–100% nucleotide identity with SStrV sequences from the GenBank database. SStrV isolates were distributed in six phylogenetic groups, including the four strains of the virus. Most varieties infected by SStrV originated from Asia, thus confirming a previous hypothesis stating that this virus originated from this continent. Absence of SStrV in commercial sugarcane in Florida also suggested that this virus has not been spread in this location, while infected plants have been present for several decades. 相似文献
2.
Jean‐Pierre Cuif Yannicke Dauphin Gilles Luquet Oulfa Belhadj Stphan Rouzire Murielle Salom Marine Cotte Andrea Somogyi Kadda Medjoubi Cedrik Lo Denis Saulnier 《Aquaculture Research》2020,51(2):506-522
Cultivated pearls frequently exhibit morphological irregularities making obvious that mineral deposition was irregularly distributed onto nucleus surface. Taking advantage of experimental cultivations with short durations (from 10 days to few months), these irregular deposits predating occurrence of the nacre were investigated in Polynesian pearls by biochemical characterizations and a series of physical methods. Diversity in the resulting data suggests that various in‐depth alterations of the biomineralization mechanism may have occurred during the grafting process, leading to diversity in the biochemical pathways to nacreous deposition. This allows a precise discussion of current views about pearl formation. The “reversed shell theory” is formally disproved through point to point comparison with development of the shell growing edge. Similarity of pearl formation with “regeneration” or “shell repair” is also discussed, emphasizing the differences between these concepts. 相似文献
3.
Luc Doyen Christophe Béné Michel Bertignac Fabian Blanchard Abdoul Ahad Cissé Catherine Dichmont Sophie Gourguet Olivier Guyader Pierre‐Yves Hardy Sarah Jennings Lorne Richard Little Claire Macher David Jonathan Mills Ahmed Noussair Sean Pascoe Jean‐Christophe Pereau Nicolas Sanz Anne‐Maree Schwarz Tony Smith Olivier Thébaud 《Fish and Fisheries》2017,18(6):1056-1072
Reconciling food security, economic development and biodiversity conservation is a key challenge, especially in the face of the demographic transition characterizing many countries in the world. Fisheries and marine ecosystems constitute a difficult application of this bio‐economic challenge. Many experts and scientists advocate an ecosystem approach to manage marine socio‐ecosystems for their sustainability and resilience. However, the ways by which to operationalize ecosystem‐based fisheries management (EBFM) remain poorly specified. We propose a specific methodological framework—viability modelling—to do so. We show how viability modelling can be applied using four contrasted case‐studies: two small‐scale fisheries in South America and Pacific and two larger‐scale fisheries in Europe and Australia. The four fisheries are analysed using the same modelling framework, structured around a set of common methods, indicators and scenarios. The calibrated models are dynamic, multispecies and multifleet and account for various sources of uncertainty. A multicriteria evaluation is used to assess the scenarios’ outcomes over a long time horizon with different constraints based on ecological, social and economic reference points. Results show to what extent the bio‐economic and ecosystem risks associated with the adoption of status quo strategies are relatively high and challenge the implementation of EBFM. In contrast, strategies called ecoviability or co‐viability strategies, that aim at satisfying the viability constraints, reduce significantly these ecological and economic risks and promote EBFM. The gains associated with those ecoviability strategies, however, decrease with the intensity of regulations imposed on these fisheries. 相似文献
4.
Marine Ollier Vincent Talle Anne‐Laure Brisset Zo Le Bihan Simon Duerr Marc Lemmens Ellen Goudemand Olivier Robert Jean‐Louis Hilbert Hermann Buerstmayr 《Plant Breeding》2019,138(1):69-81
Fusarium head blight (FHB) is a cereal disease of major importance responsible for yield losses and mycotoxin contaminations in grains. Here, we introduce a new measurement approach to quantify FHB severity on grains based on the evaluation of the whitened kernel surface (WKS) using digital image analysis. The applicability of WKS was assessed on two bread wheat and one triticale grain sample sets (265 samples). Pearson correlation coefficients between Fusarium‐damaged kernels (FDK) and WKS range from r = 0.77 to r = 0.81 and from r = 0.61 to r = 0.86 for the correlation between deoxynivalenol (DON) content and WKS. This new scoring method facilitates fast and reliable assessment of the resistance to kernel infection and shows significant correlation with mycotoxin content. WKS can be automated and does not suffer from the “human factor” inherent to visual scorings. As a low‐cost and fast approach, this method appears particularly attractive for breeding and genetic analysis of FHB resistance where typically large numbers of experimental lines need to be evaluated, and for which WKS is suggested as an alternative to visual FDK scorings. 相似文献
5.
Stacie Aarsvold Jennifer A. Reetz Jean K. Reichle Ian D. Jones Christopher R. Lamb Maria G. Evola Michele A. Keyerleber Angela J. Marolf 《Veterinary radiology & ultrasound》2015,56(3):272-277
Primary pulmonary neoplasia is relatively uncommon in cats and generally has a poor prognosis. In this multicenter, retrospective study of 57 cats with pulmonary neoplasia, the most frequent presenting signs were anorexia/inappetence (39%) and cough (37%). The pulmonary tumors were considered to be incidental findings in 9% cats. In computed tomographic (CT) images, primary pulmonary tumors appeared as a pulmonary mass in 55 (96%) cats and as a disseminated pulmonary lesion without a defined mass in two (4%) cats. Most pulmonary tumors were in the caudal lobes, with 28 (49%) in the right caudal lobe and 17 (30%) in the left caudal lobe. CT features associated with pulmonary tumors included mass in contact with visceral pleura (96%), irregular margins (83%), well‐defined borders (79%), bronchial compression (74%), gas‐containing cavities (63%), foci of mineral attenuation (56%), and bronchial invasion (19%). The mean (range) maximal dimension of the pulmonary masses was 3.5 cm (1.1–11.5 cm). Additional foci of pulmonary disease compatible with metastasis were observed in 53% cats. Pleural fluid was evident in 30% cats and pulmonary thrombosis in 12% cats. The histologic diagnoses were 47 (82%) adenocarcinomas, six (11%) tumors of bronchial origin, three (5%) adenosquamous cell carcinomas, and one (2%) squamous cell carcinoma. In this series, adenocarcinoma was the predominant tumor type, but shared many features with less common tumor types. No associations were identified between tumor type and CT features. Prevalence of suspected intrapulmonary metastasis was higher than in previous radiographic studies of cats with lung tumors. 相似文献
6.
7.
Population pharmacokinetics of methadone hydrochloride after a single intramuscular administration in adult Japanese sika deer (Cervus nippon nippon) 下载免费PDF全文
Christopher Scala Amélie Marsot Marie‐Josée Limoges Yann Locatelli Nicolas Simon Jean‐Claude Alvarez 《Veterinary anaesthesia and analgesia》2015,42(2):165-172
ObjectiveTo assess the population pharmacokinetics of methadone in deer.Study designProspective non-randomized experimental trial.AnimalsTwelve healthy adult sika deer (nine males and three females).MethodsDeer received intramuscular administration of racemic methadone hydrochloride at 0.5 mg kg−1 or 1 mg kg−1. Plasma methadone and its metabolite 2-Ethylidene-1,5-Dimethyl-3,3-Diphenyl-Pyrolidine (EDDP) concentrations were determined by validated liquid chromatography coupled to tandem mass spectrometry methods, at times 0, 30 minutes, 1, 2, 3, 4, 5, 6, 8, 12 and 24 hours. Population pharmacokinetics analysis was undertaken using a non-linear mixed effects modelling (NONMEM).ResultsA two-compartment linear disposition model best described observed time-concentration profiles of methadone and EDDP. Population parameter estimates of methadone were elimination clearance (17.3 L hour−1), metabolic clearance (34.6 L hour−1), volume of distribution of compartment 1 (216.0 L) and volume of distribution of compartment 2 (384.0 L). Population parameter estimates of EDDP were elimination clearance (121.0 L hour−1), volume of distribution of compartment 3 (1.08 L) and volume of distribution of compartment 4 (499.5 L). The total clearance and total volume of distribution of methadone and EDDP were 51.9 L hour−1, 121.0 L hour −1, 600.0 L and 500.6 L, respectively. The methadone terminal elimination half-life was 8.19 hours. No adverse effects were observed after methadone administration.Conclusions and Clinical relevanceFollowing intramuscular injection, methadone was characterized by a large total volume of distribution, high systemic clearance and intermediate terminal half-life in sika deer. 相似文献
8.
Serge MORAND Frédéric BORDES Hsuan‐Wien CHEN Julien CLAUDE Jean‐François COSSON Maxime GALAN Gábor Á CZIRJÁK Alex D GREENWOOD Alice LATINNE Johan MICHAUX Alexis RIBAS 《Integrative zoology》2015,10(5):409-423
We summarize the current knowledge on parasitism‐related invasion processes of the globally invasive Rattus lineages, originating from Asia, and how these invasions have impacted the local epidemiology of rodent‐borne diseases. Parasites play an important role in the invasion processes and successes of their hosts through multiple biological mechanisms such as “parasite release,” “immunocompetence advantage,” “biotic resistance” and “novel weapon.” Parasites may also greatly increase the impact of invasions by spillover of parasites and other pathogens, introduced with invasive hosts, into new hosts, potentially leading to novel emerging diseases. Another potential impact is the ability of the invader to amplify local parasites by spillback. In both cases, local fauna and humans may be exposed to new health risks, which may decrease biodiversity and potentially cause increases in human morbidity and mortality. Here we review the current knowledge on these processes and propose some research priorities. 相似文献
9.
10.