High night-time temperature during flowering and pod filling affects flower opening,yield and seed fatty acid composition in canola

Winter canola (Brassica napus L.) is highly sensitive to increasing temperatures during the reproductive and pod-filling stages. Although the impact of high day-time temperature stress on yield and quality has been documented in canola, similar information under high night-time temperature (HNT) stress is not available. Using six hybrids and four open-pollinated cultivars, we observed a marked shift in peak flowering towards earlier, cooler hours of the morning under HNT. Averaged across two independent experiments, the photochemical efficiency of photosystem II was significantly decreased (3%), with a significant increase in thylakoid membrane damage (13%) in the leaves of susceptible cultivars under HNT stress. Similarly, the susceptible cultivars also recorded significant reduction in biomass (34%), pod number (22%), pod weight (37%) and total seed weight (40%) per plant while the same set of agronomic traits were not affected among the tolerant cultivars. Quantitative impact of heat stress was confirmed with increased sensitivity to HNT exposure from gametogenesis until maturity resulting in a significantly higher yield loss compared to stress exposure from post-flowering till maturity. HNT significantly decreased oil concentration, but increased protein concentration and saturated fatty acid levels in seeds of the susceptible cultivars. However, HNT had no impact on the unsaturated fatty acids in both hybrids and the open-pollinated cultivars. Breeding targets based on fatty acid composition for enhancing canola seed quality may not be easily amenable due to the inconsistency documented with the compositional changes under heat stress. In summary, our findings conclude that canola hybrids are better suited to regions experiencing heat stress, compared to open-pollinated cultivars, indicating the possibility of a complete shift to hybrid canola cultivation under predicted hotter climates in the future.     

Preliminary study on antifertility activity of Calotropis procera roots in female rats

The effect of ethanolic extract of the roots of Calotropis procera has been studied in albino rats to explore its antifertility and hormonal activities. A strong antiimplantation (inhibition 100%) and uterotropic activity was observed at the dose level of 250 mg/kg (1/4 of LD(50)). No antiestrogenic activity could be detected.     

Acquired Thermo‐Tolerance and Trans‐Generational Heat Stress Response at Flowering in Rice

In rice, pre‐exposure to sublethal treatment followed by harsh lethal treatment is known to improve tolerance of different abiotic stresses at the vegetative stage within and across generations. Our major aim was to test the phenomenon of thermo‐tolerance at flowering across (trans)‐generations and within generation using rice cultivars contrasting for heat stress tolerance at flowering. To test trans‐generational response, plants were exposed to higher temperature at flowering stage and seeds obtained from previous generations were exposed to heat stress during flowering, which recorded significantly lower fertility when exposed to the same degree of stress in their subsequent generations. A pre‐acclimation to moderately high acclimating temperatures imposed over three different durations during the vegetative and initial reproductive stage showed positive response in the tolerant N22, particularly under severe heat stress (40 °C). This finding indicates the possibility of acquiring ameliorative thermo‐tolerant mechanisms at anthesis, restricted to tolerant genetic backgrounds to combat subsequent harsh conditions within the same generation. However, trans‐generational memory was ineffective in mitigating spikelet sterility losses in both tolerant and susceptible backgrounds. Rice is extremely sensitive to heat stress during flowering; hence, similar exercise across other crops of interest needs to be carried out before generalizing conclusions.     

Community water management to intensify agricultural productivity in the polders of the coastal zone of Bangladesh

Paddy and Water Environment - Most of the lands of the southern coastal zone of Bangladesh are protected from tidal flooding and storm surges by embankments constructed during the 1960s and 1970s,...     

Performance of CERES-Rice and CERES-Wheat models in rice–wheat systems: A review

Rice–wheat (RW) systems are critical to food security and livelihoods of rural and urban poor in south Asia and China, and to regional economies in southeast Australia. The sustainability of RW systems in south Asia is, however, threatened by yield stagnation or decline, and declining partial factor productivity, soil organic C and water availability. Crop models potentially offer a means to readily explore management options to increase yield, and to determine trade-off between yield, resource-use efficiency and environmental outcomes. This paper reviews the performance of CERES-Rice and CERES-Wheat in Asia and Australia in relation to their potential application towards increasing resource use efficiency and yield of RW systems.

The performance of the models was evaluated using simulated and observed data on anthesis and maturity dates, in-season LAI and growth, final grain yield and its components, and soil water and N balances from published studies across Asia and Australia, and then by computing the statistical parameters for the major characters. Over the four data sets examined for anthesis and six for maturity dates, CERES-Rice predicted those dates fairly well (normalised RMSE = 4–5%; D-index = 0.94–0.95), but over the 11 sets for grain and 4 for biomass yield, the predictions were more variable (normalised RMSE = 23% for both; D-index 0.90 and 0.76, for grain and biomass, respectively). Model performance was poorer under conditions of low N, water deficit, and low temperatures during the reproductive stages. Over the three data sets examined, CERES-Wheat predicted the anthesis and maturity dates quite well (normalised RMSE = 4–5%; D-index = 0.94–0.99), and over eight sets for grain and two sets for biomass yield the model predicted them also reasonably well (RMSE = 13–16%; D-index = 0.86–0.97). Only one study evaluated the DSSAT RW sequence model with fairly satisfactory predictions of rice and wheat yields over 20 years with adequate N, but not the long-term change in soil organic C and N. Predictions of in-season LAI and crop growth, and soil and water processes were quite limited to investigate the robustness of model processes.

Application of models to evaluate options to increase water and N use efficiency requires the ability to perform well at the margin where deficit stress begins. While both models generally perform satisfactorily under water and N non-limiting conditions, the little evidence available suggests that they do not perform well under resource-limiting situations. We recommend that the models’ key processes under the water and N limiting conditions be further evaluated urgently. The DSSAT sequence model also needs to be further evaluated against observations for a range of locations and management using data from long-term experiments in RW systems.     

New strategies for the elimination of polio from India

The feasibility of global polio eradication is being questioned as a result of continued transmission in a few localities that act as sources for outbreaks elsewhere. Perhaps the greatest challenge is in India, where transmission has persisted in Uttar Pradesh and Bihar despite high coverage with multiple doses of vaccine. We estimate key parameters governing the seasonal epidemics in these areas and show that high population density and poor sanitation cause persistence by not only facilitating transmission of poliovirus but also severely compromising the efficacy of the trivalent vaccine. We analyze strategies to counteract this and show that switching to monovalent vaccine may finally interrupt virus transmission.     

A new gene <Emphasis Type="Italic">Bph33</Emphasis>(<Emphasis Type="Italic">t</Emphasis>) conferring resistance to brown planthopper (BPH), <Emphasis Type="Italic">Nilaparvata lugens</Emphasis> (Stål) in rice line RP2068-18-3-5

The rice brown planthopper (BPH) Nilaparvata lugens (Stål) is one of the major pests of rice across Asia. Host-plant resistance is the most ecologically acceptable means to manage this pest. A rice breeding line RP2068-18-3-5 (RP2068) derived from the land race Velluthacheera is reported to be resistant to BPH populations across India. We identified a new R gene [Bph33(t)] in this line using advanced generation RILs derived from TN1 × RP2068 cross through phenotyping at two locations and linkage analysis with 99 polymorphic SSR markers. QTL analysis through IciMapping identified at least two major QTL on chromosome 1 influencing seedling damage score in seed box screening, honey dew excretion by adults and nymphal survival. Since no BPH R gene has been reported on chromosome 1, we designate this locus as a new gene Bph33(t) which accounted for over 20% of phenotypic variance. Scanning the region for candidate gene suggested two likely candidates a leucine rich repeat (LRR) gene and a heat shock protein (HSP) coding gene. Expression profiling of the two genes in the two contrasting parents and RILs showed induction of the HSP gene (LOC_Os01g42190.1) at 6 h after infestation while LRR gene did not show such induction. It is likely that the HSP represented Bph33(t).