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Navid Adnani Emmanuel Vazquez-Rivera Srikar N. Adibhatla Gregory A. Ellis Doug R. Braun Tim S. Bugni 《Marine drugs》2015,13(10):6082-6098
With respect to bacterial natural products, a significant outcome of the genomic era was that the biosynthetic potential in many microorganisms surpassed the number of compounds isolated under standard laboratory growth conditions, particularly among certain members in the phylum Actinobacteria. Our group, as well as others, investigated interspecies interactions, via co-culture, as a technique to coax bacteria to produce novel natural products. While co-culture provides new opportunities, challenges exist and questions surrounding these methods remain unanswered. In marine bacteria, for example, how prevalent are interspecies interactions and how commonly do interactions result in novel natural products? In an attempt to begin to answer basic questions surrounding co-culture of marine microorganisms, we have tested both antibiotic activity-based and LC/MS-based methods to evaluate Micromonosporaceae secondary metabolite production in co-culture. Overall, our investigation of 65 Micromonosporaceae led to the identification of 12 Micromonosporaceae across three genera that produced unique metabolites in co-culture. Our results suggest that interspecies interactions were prevalent between marine Micromonosporaceae and marine mycolic acid-containing bacteria. Furthermore, our approach highlights a sensitive and rapid method for investigating interspecies interactions in search of novel antibiotics, secondary metabolites, and genes. 相似文献
3.
Tamas D. Ambrisko Arnon Gal Jesus N. Sarol Ashley Mitek Christina Braun 《Veterinary anaesthesia and analgesia》2021,48(2):205-212
ObjectiveTo evaluate the influence of reservoir bag types, volumes and previous use on the peak pressures (Pmax) and the times to develop 30 cmH2O pressure (P30) within a nonrebreathing system with a closed adjustable pressure-limiting (APL) valve.Study designIn vitro study using three-way factorial design with repeated measure on one factor.SubjectsA total of 75 new anesthesia reservoir bags (five types, three volumes, five bags from each type × volume). The bag types were reusable latex (RL), disposable latex (DL) and three disposable neoprene (DN-1, DN-2 and DN-3).MethodsEach bag was tested three times (treatments): new, after prestretching and 1 week later. The bags were attached to a Bain system and anesthesia machine with closed APL valve and patient port with O2 flow 2 L minute–1 until Pmax was reached. The Pmax and time to reach P30 values were determined from recorded pressure traces. General linear mixed model analysis was used to examine the effects of bag type, volume and treatment. One-sided 95% upper prediction limits of Pmax were calculated to test the null hypothesis that predicted Pmax of new bags would be ≥ 50 cmH2O for each factor combination.ResultsRL bags were the least and DN-3 bags were the most compliant. Prestretching increased compliance in all bag types. Smaller bags of RL, DL and DN-1 were less compliant than larger ones. The predicted Pmax values were < 50 cmH2O only for DN-3 bags after prestretching. The time to reach P30 was critically low when using 0.5 L bags (median 17 seconds).Conclusions and clinical relevanceTo minimize the risk of barotrauma, highly compliant reservoir bags (e.g. DN-3) are recommended and reusable bags should be avoided. Bags should be prestretched before first use, 0.5 L bags should be avoided and fresh gas flow minimized. 相似文献
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M. v. Cotta M. v. Cotta Mördes v. Pannewib Robert Rühn Paur v. Stelzl v. Deltheim Schulze Q. v. Mikleben Lreiherr v. Lflanteuffel Lreiherr v. Berg Qeitel M. Lreiherr v. Berg v. Michael H. C. Bär Baer A. Chieriod Berger Rettftadt Gekner Wlrici Lreiherr v. Schmerzing Sprenger Pommrich v. Strune Matufchka v. E. Q. Brunft Rnaudt Mördlinger Rnipping v. Mangenheim C. A. Baum L. Mehlborn G. v. Stieglik H. v. Biegefar C. Grebe v.n Baumbach Braun R. Blohmer 《European Journal of Forest Research》1865,9(1):322-324
Ohne Zusammenfassung
f. f?chf. Dberforftmeifter.
f. bahr. Regierungs- und Forftrath von Mürzburg.
f. preuk. Dberforftmeifter aus Breslau.
f. f?chf. Dberforftmeifter.
f. bahr. Regierungs- und Forftrath von Augsburg.
f. bahr. Regierungs- und Forftrath aus Regensburg.
Dberj?germeifter von Braunfchweig.
f. bahr. Regierungs- und Forftrath aus München.
f. f?chf. Dberf?rfter.
f. f?chf. Dberforftmeifter.
f. f?chf. Dberforftrath.
herz. braunfchweig. Forftmeifter von Blanfenburg.
Direftor der fürftlich Cfterhazh’fchen Herrfchaft Spoly Parsto.
fürftl. fondershauf. Dberfortmeifter.
f. bahr. Redierf?rfter don Schmarzenbach.
f. preuk. Dberf?rfter.
f. f. Forftrath aus Brekburg.
f. preuk. Dberf?rfter von Altruppin.
f. hann?v. Forftmeifter von Rienburg.
Forftmeifter in preuk. Schleften.
f. preuk. Dberforftmeifter von Berlin.
herz. aftenburg. Dberfortmeifter.
f. preuk. Dberf?rfter.
f. f?chf. Dberf?rfter.
f. ruff. Forftfomiff?r aus Marfchau.
f. preuk. Forftinfpeftor.
f. f?chf. Forftinfpeftor.
grokh. meimar. Forftinfpeftor von Martfuhl.
f. mürttb. Dberf?rfter und Brofeffor aus Hohenheim.
f. hannov. Forftmeifter aus Hammeln.
Dberforftmeifter aus Sotha.
herz. altenburg. Dberf?rfter aus Hummelshain.
herz. altenburg. Revierf?rfter aus Milchwig.
herz. altenburg. geheimer Finanzrath und Dberforftmeifter.
herz. altenb. Dberforftmeifter aus Hummelshain.
grokh. f?chf. geheimer Dberforftrath aus Qifenach.
herz. naffau. Dberforftrath aus Miesbaden.
grokh. heff. Dberforftfefret?r aus Darmftadt.
f. f?chf. Dberforftmeifter. 相似文献
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Ohne Zusammenfassung 相似文献
9.
False thrombocytopenia may result from platelet aggregation, especially in feline ethylenediamine tetra-acetic acid (EDTA) blood specimens. Citrate, theophylline, adenosine and dipyridamole (CTAD) was added to 46 feline EDTA specimens to test its anti-aggregation action. Platelet aggregation was estimated from blood films and a complete blood count was performed with a Sysmex XT-2000iV analyser. Platelet aggregation score was >2 in 11/46 EDTA tubes and only in one EDTA+CTAD specimen. The platelet count was higher in all CTAD-supplemented tubes except one, medians measured by cytometry being 225.5 × 10(9)/l and 249.0 × 10(9)/l in EDTA and EDTA+CTAD, respectively (P = 0.007). Adding CTAD had statistically and analytically significant but moderate effects on other blood variables, the most intense variations being observed for reticulocytes (about 3% higher in EDTA specimens) and reticulocyte indexes. Addition of CTAD to EDTA when sampling feline blood is a useful option to reduce platelet clumping. 相似文献
10.
Anaplasmosis in cattle in Switzerland 总被引:1,自引:0,他引:1