Histopathology of Experimental Schistosoma bovis Infection in Goats

The inflammatory host response to Schistosoma bovis in young goats was studied at necropsy by light microscopy 34 weeks after primary exposure to 3,000 cercariae (group B, n=6), 34 weeks after primary exposure to 3,000 cercariae followed by challenge with 2,500 cercariae at week 17 (group C, n=5), and 17 weeks after primary exposure to 2,500 cercariae, given on week 17 of the experiment (group D, n=6). Three goats served as uninfected controls. The faecal egg output had been minimal for 17 weeks prior to necropsy in groups B and C and only for the last 2 weeks in group D.Histological studies were carried out on the small intestine, liver, lung and spleen, and tissue egg counts were performed. In sections of the small intestine and liver, a panel of histopathological variables were quantitated to characterize the host response and differences between groups of animals were evaluated with one way analysis of variance. The mean tissue egg count in the small intestine was slightly but not significantly higher in group C than group B and about twice as high in group D (D vs B or C p<0.01). Group means of numbers of inflammatory foci per section of gut wall corresponded well with those of tissue egg counts, suggesting that the rate of inflammatory destruction of eggs did not differ markedly between the groups. Egg material was less commonly seen in granulomas of the small intestine in group B than in group D (p<0.01), suggesting lower passage of eggs through the gut wall during the later than during the earlier phase of patent primary infection. The frequency of eosinophil-rich hepatic inflammatory foci was much higher in group D than in the other groups (D vs B p<0.05, D vs C p< 0.01), and coincided with a high degree of blood eosinophilia in this group at the time of sacrifice. Challenged goats showed a significantly higher frequency of markedly fibrotic inflammatory foci in the liver and of liver granulomas with a marked giant cell component than goats of the other groups. Hepatic portal fibrosis was least prominent in animals with 17- week- old primary infections, implying a possible relation between this change and duration of infection.     

Investigations on the Occurrence of Hereditary Diseases in the Danish Cattle Population 1989-1991

Agerholm, J.S., A. Basse and K. Christensen: Investigations on the occurrence of hereditary diseases in the Danish cattle population 1989-1991. Acta vet. scand. 1993, 34, 245-253.– The methods of the Danish Bovine Genetic Disease Programme are outlined, and the results obtained during the first 3 years in function are described. The most common disease reported was spinal muscular atrophy in calves of the Red Danish Dairy breed with 312 reports. Necropsy was performed on 162 cases, and spinal muscular atrophy was diagnosed in 82 of these. Bovine progressive degenerative mye-loencephalopathy, rectovaginal constriction, syndrome of arthrogryposis and palatoschisis, hereditary chondrodysplasia (2 different types), syndactylism, epitheliogenesis imperfecta, and osteogenesis imperfecta was diagnosed with 1 case each. Lethal trait A46 was diagnosed in 4 calves. Some of these diseases have not previously been described in Denmark, and epitheliogenesis imperfecta was for the first time diagnosed in the Hereford breed. Chromosome translocation 1/29 was detected in the Blonde d’Aquitaine (BAQ), Limousine, and Red Danish Dairy breed. The aberration occurred frequently in BAQ. Furthermore, a complex chromosome translocation t(l;8;9)(q45;ql3;q26) was detected in the Red Danish Dairy breed.     

An Epidemiologic and Economic Study of Respiratory Diseases in Two Conventional Danish Swine Herds. I: Prevalence of Respiratory Lesions at Slaughter and Their Effects on Growth

A total of 578 slaughter pigs from 2 Danish conventional farrow-to-finish operations (Herds A and B) were followed from an age of 14 days to slaughter. Pigs were weighed at 3 weeks intervals and at slaughter and an extended post mortem examination of the plucks was done. Comparison of growth rates in pigs with and without specific types of lesions by the t-test and those with multiple lesions with regression models demonstrated that Mycoplasma-like pneumonia, complicated pneumonia, anterio-ventral pleuritis, fissures and atrophic rhinitis significantly reduced mean daily gain and increased the time required to reach slaughter weight. The total impact of the lesions in Herd A was an estimated reduction in mean daily gain of 27 grams and a 2 day increase in the interval from 14 days of age until slaughter (MDG14). Decreases in MDG14 in Herd B were more substantial, 98 grams and 16.7 days. Reductions in mean daily gains during the interval from the fourth weighing until slaughter were 31 grams in Herd A and 137 grams in Herd B. Chronic dorso-caudal and parietal pleuritis, without other lesions present, had no significant adverse effects on growth rates in either herd. Interactions between lesions did not significantly alter the estimates. The R² values obtained for the regression models showed that the presence, absence or extent of lesions at slaughter explained only 13-27% of the variations in growth rates in the 2 herds.     

Evaluation of the effects of enzootic pneumonia in pigs on weight gain and days to slaughter under New Zealand conditions

The effects of enzootic pneumonia on average daily weight gain and number of days taken to reach slaughter weight was studied in 333 bacon-weight pigs. Each 1% of lung volume affected by enzootic pneumonia was shown to reduce average daily gain by 2.2 grams and increase the numbers of days to slaughter by 0.61 days. The cost of these reductions in performance was calculated to be about NZD 1 for each 1% of lung affected.     

Using laser to measure stem thickness and cut weed stems

Summary Stem thickness of the weed Solanum nigrum and the crop sugarbeet was determined with a He–Ne laser using a novel non‐destructive technique measuring stem shadow. Thereafter, the stems were cut close to the soil surface with a CO₂ laser. Treatments were carried out on pot plants, grown in the greenhouse, at two different growth stages, and plant dry matter was measured 2–5 weeks after treatment. The relationship between plant dry weight and laser energy was analysed using two different non‐linear dose–response regression models; one model included stem thickness as a variable, the other did not. A binary model was also tested. The non‐linear model incorporating stem thickness described the data best, indicating that it would be possible to optimize laser cutting by measuring stem thickness before cutting. The general tendency was that more energy was needed the thicker the stem. Energy uses on a field scale are discussed.     

Manipulation of cattle growth to target carcass quality

Two trials were conducted to determine the effects of breed type, backgrounding program, and backfat end point (USBFEP) as determined by ultrasound on the performance and carcass characteristics of feedlot steers. Trial 1 utilized 144 large-framed Charolais- (304.6 +/- 16.3 kg) and 144 medium-framed Hereford-cross steers (294.8 +/- 20.9 kg). Trial 2 utilized 88 medium-framed Angus (289.5 +/- 15.0 kg), 88 large-framed Charolais- (299.8 +/- 17.7 kg), and 88 medium-framed Hereford-cross (291.1 +/- 20.9 kg) steers. Within breed type, short- (70 d) or long-term (126 d) backgrounding programs (Trial 1) and 6- or 12-mm USBFEP (Trials 1 and 2) were assigned. In both trials, carcass size was greater (P < 0.05) for the large-framed Charolais-cross steers, longer backgrounding, and fatter USBFEP treatments. Lean meat yield was greater (P < 0.05) for Charolais- than Hereford-cross steers (Trial 1) and for the leaner USBFEP (Trials 1 and 2). Marbling was greater (P < 0.05) for Angus- (Trial 2) and Charolais- (Trials 1 and 2) than Hereford-cross steers (Trials 1 and 2) and for the fatter end point (Trials 1 and 2). It was concluded that breed type, backgrounding program, and USBFEP were effective in altering growth and could be used to target carcass traits of interest for value-based marketing programs.     

Studies on the prevalence of Riemerella anatipestifer in the upper respiratory tract of clinically healthy ducklings and characterization of untypable strains

A total of 44 bacterial strains obtained from 49 clinically healthy ducklings of different ages originating from four different farms were identified as members of the species Riemerella anatipestifer (RA) using conventional biochemical test methods. Numerical analysis of the whole-cell fatty acid patterns of these isolates resulted in two different clusters, one of which showed a similar pattern to that of the type strain of RA. Strains having a different fatty acid methyl esters (FAME)-profile (cluster II) were designated R. anatipestifer-like (RA-like). Sequencing of 16S rRNA genes of RA-like field isolates revealed 99% identity to RA. The significance of these observations are discussed. The present findings document for the first time that RA seems to represent a normal part of the pharyngeal flora of healthy Pekin ducks.