Immunohistochemical Expression of Growth Factors in the Follicular Wall of Normal and Cystic Ovaries of Sows

The expression of growth factors was evaluated immunohistochemically in normal and cystic ovaries of sows. The immunohistochemically stained area (IHCSA) was quantified by image analysis to analyse the expression of these proteins in the follicular wall of secondary, tertiary and cystic follicles. IGF‐I immunoreactivity was strong in the granulosa cell layer (GC), moderate in the theca interna (TI) and mild in the theca externa (TE) of the normal follicles. There was severe reduction of the labelling to IGF‐I in the GC of the follicular and luteinized cysts. In the normal follicles, the reactivity for IGF‐II was very similar to pattern noted in IGF‐I. There was reduction of the IHCSAs in the GC of the follicular and luteinized cysts, but the decrease was not significant. The staining of the IGF‐II in the TI and TE of the cysts was increased, in comparison with normal follicles. The IHCSAs for VEGF were higher in the GC and TE of the normal follicles in contrast to TI, but this difference was noted only in the tertiary follicle. The VEGF reactivity increased in the GC of the cysts, in relation to normal follicles. The results of the current study show that the formation of ovarian cysts in sows is associated with alterations in the immunohistochemical expression of some growth factors.     

Degradation and binding of atrazine in surface and subsurface soils

Understanding the dissipation rates of chemicals in unsaturated and saturated zones of subsurface soils will help determine if reductions of concentrations to acceptable levels will occur. Chemical properties and microbial biomass and activity were determined for the surface (0-15 cm), lower root (50-105 cm), and vadose (175-220 cm) zones in a Huntington silty clay loam (Fluventic Hapludoll) collected from an agricultural field near Piketon, OH. The rates of sorption, mineralization, and transformation (formation of bound residues and metabolites) of atrazine were determined. Microbial activity was estimated from the mineralization of (14)C-benzoate. We observed decreased levels of nutrients (total organic carbon, N, and P) and microbial biomass with depth, while activity as measured with benzoate metabolism was higher in the vadose zone than in either the surface or the root zones. Sorption coefficients (K(f)) declined from 8.17 in the surface to 3.31 in the vadose zone. Sorption was positively correlated with organic C content. Rates of atrazine mineralization and bound residues formation were, respectively, 12-2.3-fold lower in the vadose than in the surface soil. Estimated half-lives of atrazine ranged from 77 to 101 days in the surface soil, but increased to over 900 days in the subsurface soils. The decreased dissipation of atrazine with increasing depth in the profile is the result of decreased microbial activity toward atrazine, measured either as total biomass or as populations of atrazine-degrading microorganisms. The combination of reduced dissipation and low sorption indicates that there is potential for atrazine movement in the subsurface soils.     

Genese und Ökologie innerstädtischer Böden aus Bauschutt

Formation and ecology of rural soils from debris Within 30 years Rendzinas have developed on habitats of debris and rubbles in West-Berlin by accumulation of organic substances, carbonate impoverishing, slightly beginning acidity, and biogenic structuring. Their conditions for water, air, and nutrients were investigated for 2 years.     

Konzeptbodenkarten für den städtischen Raum

Conception soil maps for urban areas The demand for soil maps increased strongly during the last years. This concerns urban soils, too. Conception soil maps constructed from a lot of basic informations (basic maps and point data) are the alternative to traditional soil mapping, which is expensive in time and money for urban areas. Further informations are to evaluate maps from these conception soil maps. Using graphic information-systems is advantageous for construction and evaluation.     

Genesis of a So-called Ferrolysed Soil of Bangladesh

It was postulated by Brinkman (1970) that low clay contents in the surface horizon of paddy soils (Planosols) are caused by clay destruction through ferrolysis. In an effort to test this, a rice profile from the site of Brinkman's Eutric Planosol (Salna, Bangladesh) and a neighbouring forest soil (Dystric Cambisol) of the same parent material and particle-size distribution (Bhawal National Park) were investigated. The micro-structure, particle-size distribution, mineral contents, pedogenic oxides, contents of Zr, Ti and K of the fine earth and individual fractions and also the exchangeable ions were studied. The clay fraction of both soils were chloritised by interlaying of hydroxy-Al in the smectite interlayers in the surface horizons and got stabilized. As a result CEC showed some decrease. The forest profile has a loose and uniform brown-yellow surface horizon and is relatively rich in coarse sand at 62–75 cm depth which might be caused by a strong termite activity as observed during sampling. On the contrary, the frequent and alternate reducing and oxidizing conditions have resulted in gleying the top soil and forming iron-oxide films on the surface of aggregates during dry periods in the paddy soil. An evaluation of all results shows that a little clay destruction and an enrichment of silt in the top soil through vertisol formation and also through primary parent material stratification were the reasons for low clay content in epipedons. In forest soil there was a little clay migration, while the epipedon of the paddy soil had low clay in addition, because of selective erosion during heavy rains following the destruction of structure by puddling. The ferrolysis as a dominant factor for a clay-low epipedon is improbable, because the clay loss of the neighbouring forest soil can not be explained by ferrolysis.