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Manjree Agarwal Suresh Walia Swaran Dhingra Bhupinder P
S Khambay 《Pest management science》2001,57(3):289-300
Fresh rhizomes of Zingiber officinale (ginger), when subjected to steam distillation, yielded ginger oil in which curcumene was found to be the major constituent. The thermally labile zingiberene‐rich fraction was obtained from its diethyl ether extract. Column chromatography of ginger oleoresin furnished a fraction from which [6]‐gingerol was obtained by preparative TLC. Naturally occurring [6]‐dehydroshogaol was synthesised following condensation of dehydrozingerone with hexanal, whereas zingerone and 3‐hydroxy‐1‐(4‐hydroxy‐3‐methoxyphenyl)butane were obtained by hydrogenation of dehydrozingerone with 10% Pd/C. The structures of the compounds were established by 1H NMR, 13C NMR and mass (EI‐MS and ES‐MS) spectral analysis. The test compounds exhibited moderate insect growth regulatory (IGR) and antifeedant activity against Spilosoma obliqua, and significant antifungal activity against Rhizoctonia solani. Among the various compounds, [6]‐dehydroshogaol exhibited maximum IGR activity (EC50 3.55 mg ml ?1) while dehydrozingerone imparted maximum antifungal activity (EC50 86.49 mg litre?1). © 2001 Society of Chemical Industry 相似文献
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The effect of serum from horn cancer affected bullocks and cows on E-rosetting capacity of peripheral blood lymphocytes (PBL) from unaffected control animals was examined. The E-rosette counts were made using 2-aminoethyl isothiouronium bromide (AET) treated sheep red blood cells. A significant decrease in the percentage of EAET rosette forming cells was noticed when PBL were incubated with 50 per cent serum from animals affected with horn cancer. However, no such effect was noticed when PBL were treated with 50 per cent serum from unaffected control animals. A linear relationship was observed between percentage of EAET rosette forming cells of animals affected with horn cancer and E-rosette inhibitory activity of the corresponding serum on PBL from control animals. 相似文献
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K. ShankarganeshSuresh Walia Swaran Dhingra B. SubrahmanyamS. Ramesh Babu 《Pesticide biochemistry and physiology》2012,102(1):86-90
Resistance in Spodoptera litura (Fabricius) has been attributed to enhanced detoxification of insecticides by increased levels of esterases, oxidases and/or glutathione S-transferases. Enzyme inhibiting insecticide synergists can be employed to counter increased levels of such enzymes in S. litura. Dihydrodillapiole induced synergism of pyrethroid toxicity was examined in the laboratory-reared third instar larval population of S. litura collected in Delhi (susceptible), and Guntur (resistant) region of Andhra Pradesh, India. The Guntur population was found to be 7.04 and 10.19 times resistant to cypermethrin and lambdacyhalothrin, respectively. The activity of cypermethrin, lambdacyhalothrin and profenophos against susceptible and resistance populations of S. litura, was gradually increased when used along with a plant-derived insecticide synergist dihydrodillapiole. The α-naphthyl acetate hydrolysable esterase activity in Delhi population was less as compared to the Guntur population. Resistance associated esterases in Delhi population were inhibited by pre-treatment with dihydrodillapiole. The esterase level in insect was instantly reduced initially, sustained for about 3 h and equilibrated at 4 h post treatment. The esterase activity of Guntur population was increased to 1.28 μmoles/mg/min at 2 h post treatment and subsequently reduced to lower than 0.70 μmoles at 4-12 h post treatment. The variation in esterase activity is suggestive of its homeostatic regulation in test populations. Dihydrodillapiole thus caused significant reduction of resistance in S. litura to cypermethrin, lambda cyhalothrin and profenophos. 相似文献
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Randhawa SS Dua K Randhawa CS Randhawa SS Munshi SK 《Veterinary research communications》2008,32(8):599-608
The effect of biotin supplementation on various foot lesions and hoof ceramide composition of toe (wall) and sole portions
of hooves was studied in crossbred dairy cattle. Biotin supplementation was done for five months in 14 cattle at a farm and
the other 14 animals kept as control. A significant decline was observed in heel erosions and sole avulsions along with total
disappearance of white line fissures and double soles in the biotin supplemented cattle resulting in decrease in the overall
disease score. Thin layer chromatographs of the hoof lipids revealed 11 types of ceramides in sole lipids and 6 types of ceramides
in toe (wall) lipids. The ceramides were typed and identified according to their Rf values. A qualitative increase in the
density of thin layer chromatographs of sole lipids was observed in biotin supplemented cattle whereas a non-significant difference
in density of thin layer chromatographs of toe lipids was observed after supplementation of biotin. 相似文献
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Haq Shamshad Ul Kumari Deepa Dhingra Prerna Kothari S. L. Kachhwaha Sumita 《Journal of Crop Science and Biotechnology》2021,24(3):279-292
Journal of Crop Science and Biotechnology - India has a diverse range of agro-ecological conditions which support the cultivation of different rice varieties differing in the adaptation which is so... 相似文献
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A 60% azadirachtin-A concentrate has been obtained through repeated precipitation with hexane from a methanolic solution of a 20% concentrate. Azadirachtin-A (90%) has been obtained by medium-pressure liquid chromatography of the 60% concentrate with an RP-18 column and a methanol + water (1 + 1 by volume) solvent system. Catalytic hydrogenation of the 60 and 90% azadirachtin concentrates yielded the corresponding tetrahydroazadirachtin concentrates. Dihydroazadirachtin and tetrahydroazadirachtin formed during the first 5 h of hydrogenation were identified by LC-ESI-MS on the basis of their unique mass fragmentation pattern. The efficacy of tetrahydroazadirachtin concentrates in inhibiting the feeding and growth of Helicoverpa armigera (Hübner) larvae has been compared with that of azadirachtin concentrates. They were in general more active and deterred larvae from feeding at all concentrations. Tetrahydroazadirachtin-A (90%) and azadirachtin-A (90%) with respective IC(50) values of 280 and 390 mg L(-1) were effective as insect growth regulators, while tetrahydroazadirachtin-A (90%) displayed higher antifeedant activity (AI(50) 14 mg L(-1)) against the test insect. 相似文献
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SUMMARY The level of thymus-derived lymphocytes was assessed by determining the per cent of cells which formed non-immune rosettes with 2-Aminoethyl isothiouronium bromide treated sheep erythrocytes in the peripheral blood of 20 (14 bullocks and 6 cows) histopathologically confirmed cases of bovine squamous cell carcinoma of horn (horn cancer) and an equal number of age-matched control animals. A significantly marked (P < 0.01) depression in the per cent erythrocyte-rosette forming cell count was observed in horn cancer affected bullocks and cows as compared to the values in unaffected control animals. The decrease in the level of these cells was comparatively more marked in animals which were clinically in advanced stages of the disease. 相似文献
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P N Dhingra V M Mahajan 《Comparative immunology, microbiology and infectious diseases》1991,14(3):235-241
Two locally isolated strains of Chlamydia psittaci from faeces of apparently healthy sheep and conjunctiva of Holstein heifers suffering from conjunctivitis and pneumonia were used for ocular experimental studies in 7 Rhesus monkeys. Titrated (ELD50 10(3.6)/0.2 ml and 10(4.5)/0.2 ml) partially purified yolk sac suspension of each strain was instilled onto conjunctiva of each animal. The clinical and laboratory studies were followed till 8 weeks post-infection. Both the strains produced trachoma like follicles (0.3-0.5 mm dia) preferably on lower conjunctiva between 2-4 weeks of post-infection. Pannus was not observed in any of the experimental eye. Laboratory studies revealed a good correlation between reisolation, fluorescent antibody test and cytology between 2-4 weeks of post-infection. Highest complement fixing antibody titre 1:32 was recorded in 4 out of 7 animals. The studies indicated that both the animal strains had no barrier across ocular tissues in monkeys, vis-à-vis human conjunctiva. 相似文献