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Shantharaj Deepak Sathyanarayana Niranjan-Raj Shekar Shailasree Ramachandra K. Kini Wilhelm Boland Hunthrike S. Shetty Axel Mithfer 《Physiological and Molecular Plant Pathology》2007,71(1-3):96-105
The synthetic 1-oxo-indanoyl-l-isoleucine methyl ester (Ind-Ile-Me) represents a highly active mimic of octadecanoic phytohormones, which are involved in plant defenses against pathogens and pests. Seed treatments and foliar spray with Ind-Ile-Me were tested for induced resistance against downy mildew disease caused by the phytopathogenic oomycete Sclerospora graminicola in pearl millet (Pennisetum glaucum) under greenhouse and field conditions. Under greenhouse conditions, a 50% protection level was achieved after seed treatment. Seed treatment in combination with foliar spray resulted in 60% protection. The induction of resistance was correlated with the enhanced activities of defense-related proteins such as phenylalanine-ammonia-lyase, peroxidase, and enhanced level of hydroxyproline-rich glycoproteins. Under field conditions, a maximum protection of 62% was recorded upon seed treatment along with foliar spray. Hence, it infers that Ind-Ile-Me can be used as a valuable protection compound at least in downy mildew disease management. 相似文献
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Sharma Anuj Ference Christopher M. Shantharaj Deepak Baldwin Elizabeth A. Manthey John A. Jones Jeffrey B. 《European journal of plant pathology / European Foundation for Plant Pathology》2022,162(1):163-181
European Journal of Plant Pathology - Citrus canker (CC) is an economically important bacterial disease caused by Xanthomonas citri subsp. citri (Xcc). There is variability in resistance to CC... 相似文献
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Girigowda Manjunatha Shantharaj Deepak Prashanth N. Geetha Sathyanarayana Niranjan-Raj Ramachandra K. Kini Hunthrike Shekar Shetty 《Physiological and Molecular Plant Pathology》2009,74(2):191-198
Priming of pearl millet seedlings with nitric oxide (NO) donors sodium nitroprusside (SNP) and S-nitrosoglutathione (SNOG) induced hypersensitive reactions (HR) and accumulation of Proline/Hydroxyproline-rich glycoprotein (P/HRGP) during infection by downy mildew pathogen Sclerospora graminicola. Such defense responses were specifically altered by concentration of NO donors resulting in the modulation of endogenous NO in seedling tissues. The stoichiometric interactions of NO and hydrogen peroxide (H2O2) when followed in relation to HR and P/HRGP accumulation, the degree of defense response varied with H2O2 level, the latter being largely influenced by NO concentration. Therefore, balancing NO and H2O2 is vital for optimum expression of defense responses for imparting disease resistance. 相似文献
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Girigowda Manjunatha Sathyanaraya Niranjan‐Raj Geetha N Prashanth Shantharaj Deepak Kestur N Amruthesh Hunthrike S Shetty 《Pest management science》2009,65(7):737-743
BACKGROUND: The nature and durability of resistance offered by chitosan and the involvement of nitric oxide (NO) in chitosan‐induced defence reactions in pearl millet against downy mildew disease were investigated. RESULTS: It had previously been reported that chitosan seed priming protected pearl millet plants against downy mildew disease. Further elucidation of the mechanism of resistance showed that chitosan seed priming protects the plants systemically. A minimum 4 day time gap is required between the chitosan treatment and pathogen inoculation for maximum resistance development, and it was found to be durable. Chitosan seed priming elevated NO accumulation in pearl millet seedlings, beginning from 2 h post‐inoculation, and it was found to be involved in the activation of early defence reactions such as hypersensitive reaction, callose deposition and PR‐1 protein expression. Pretreatment with NO scavenger C‐PTIO and nitric oxide synthase (NOS) inhibitor L‐NAME before pathogen inoculation reduced the disease‐protecting ability of chitosan, and defence reactions were also downregulated, which indicated a possible role for NO in chitosan‐induced resistance. CONCLUSION: Protection offered by chitosan against pearl millet downy mildew disease is systemic in nature and durable. Chitosan‐induced resistance is activated via NO signalling, as defence reactions induced by chitosan were downregulated under NO deficient conditions. Copyright © 2009 Society of Chemical Industry 相似文献
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Shantharaj Deepak Sekhar Shailasree Neerakkal Sujeeth Ramachandra K. Kini Axel Mithöfer Shekar H. Shetty 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(1):77-85
Proline/hydroxyproline-rich glycoprotein (P/HRGP) level in pearl millet genotypes resistant to downy mildew increase after
inoculation with the oomycete pathogen Sclerospora graminicola. Using purified P/HRGPs from pearl millet cell walls, polyclonal antibodies (Pab-P/HRGP) were raised in rabbit. Based on this
antiserum, an enzyme immunoassay was developed that displays a linearity detection range from 0.01 to 10 μg P/HRGP. Western
blot analysis, confirming the induction of three marker P/HRGPs in the infected resistant genotype, and immunocytochemical
studies on P/HRGP localization either in epidermal peelings or in suspension-cultured cells demonstrated the specificity of
the antiserum. Besides its characterization, Pab-P/HRGP was employed to screen various genotypes of pearl millet for fast,
sensitive and specific detection of induced P/HRGPs upon infections. The results presented are discussed with presumed importance
to downy mildew disease and the use of this new antiserum in pearl millet screening for disease resistance. 相似文献
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