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Genomic characterization using nonradioactive probes, polymerase chain reaction with degenerate primers for whitefly transmitted geminiviruses and nucleotide sequencing were used to describe a new bipartite geminivirus, associated with dwarfing and leaf curling of tomatoes and peppers in Jamaica. Partial DNA-A and DNA-B clones were obtained. DNA sequence analysis showed that tomato and pepper samples have a similar geminivirus associated with them. Nucleotide sequence identity > 92% between the common regions of DNA-A and DNA-B confirmed the bipartite nature of the Jamaican geminivirus isolates. Nucleotide sequence comparisons of DNA-A and DNA-B with those of geminiviruses representing the major phylogenetic groups of Western Hemisphere geminiviruses showed the greatest similarity to potato yellow mosaic virus and members of the Abutilon mosaic virus cluster of geminiviruses. This new virus is given the name tomato dwarf leaf curl virus (TDLCV) because of the dwarfing and leaf curling symptoms associated with infected tomato plants. Polymerase chain reaction and Southern hybridization showed mixed infections of TDLCV with tomato yellow leaf curl virus from Israel in 16% of the field samples of tomatoes and peppers. 相似文献
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A study on the compression behavior of spacer fabrics designed for concrete applications 总被引:1,自引:0,他引:1
Spacer fabrics have been used in many areas varying from medical applications to protection applications. Especially the three
dimensional characteristic of spacer fabrics presents different opportunities for special applications. The compression resistant
characteristic of spacer fabrics is one of their main properties. In this research the compression behaviour of spacer fabrics
designed for concrete applications has been investigated. The effects of some parameters such as spacer yarn material, pattern
and threading on the compression behaviour of spacer fabrics have been studied. According to the test results it was found
that the material, pattern and the threading of spacer yarns are important parameters for the compression characteristics
of spacer fabrics. It was also observed that the location angle of spacer yarn and the amount of the spacer yarns influence
the compression behaviour of spacer fabrics. 相似文献
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Plasma kinetics of diphenylhydantoin in dogs and cats 总被引:2,自引:0,他引:2
D B Roye E E Serrano R H Hammer B J Wilder 《American journal of veterinary research》1973,34(7):947-950
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D. Van Roye N. H. Bonneau L. Breton E. Teuscher 《The Canadian veterinary journal. La revue veterinaire canadienne》1981,22(8):248-251
Multiple osteochondromatosis in a female Boxer 相似文献
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I. I. Amarakoon M. E. Roye R. W. Briddon † I. D. Bedford J. Stanley 《Plant pathology》2008,57(3):417-426
The molecular and biological characterization of a begomovirus infecting the common weed Macroptilium lathyroides from Jamaica are reported. The virus showed 92% sequence identity to an isolate of Macroptilium yellow mosaic virus (MaYMV) from Cuba, but was distinct from the two other begomoviruses isolated from M. lathyroides , namely Macroptilium yellow mosaic Florida virus (80% identity) and Macroptilium mosaic Puerto Rico virus (68% identity). Hence, the Jamaican begomovirus was considered an isolate of MaYMV and called Macroptilium yellow mosaic virus -[Jamaica] (MaYMV-[JM]). In infectivity studies using cloned DNA-A and DNA-B genomic components, MaYMV-[JM] infected red kidney bean ( Phaseolus vulgaris ) and produced mild symptoms in Scotch Bonnet pepper ( Capsicum chinense ), but did not infect cabbage ( Brassica oleracea ). This information has implications for the development of strategies to control begomovirus diseases in Jamaica and elsewhere. 相似文献
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