不同抗性种质资源对玉米弯孢霉叶斑病菌产生的细胞壁降解酶活性的影响

 It was found that activities of cellulase and pectolytic enzyme produced from Curvularia lunata, were significantly affected when the pathogen inoculated on various maize germplasms with different resistant levels. Isolate 98-07, which inoculated on resistant germplasms for single generation, was induced relatively higher average cellulase activity (7.99 U/mL) than that induced on susceptible germplasms (4.91 U/mL). By consecutively inoculating low pathogenicity isolates C107 and WS18 on resistant inbred line Shen135 for 5 generations, we found that their pathogenicity improved generally as the increase of generations, and simultaneously their cellulase activities were also enhanced (C107:32.0%;WS18:72.2%). The data indicated that there is a significant correlation between isolate cellulase activities and the changes of its pathogenicity (C107:r=0.916;WS18:r=0.905) induced specifically on resistant germplasms.     

玉米弯孢叶斑病菌致病类型分化研究

 利用鉴别寄主和同工酶技术,对采自黑龙江、吉林、辽宁和河北4省玉米弯孢叶斑病菌菌株的致病类型进行分析。研究表明,鉴别寄主技术将26个菌株的致病类型划分为4种类型,其中强和中等致病类型主要分布在辽宁省瓦房店市、吉林省公主岭市和河北省保定市。玉米弯孢叶斑病菌在酯酶、苹果酸脱氢酶、过氧化物酶、多酚氧化酶、超氧化物歧化酶同工酶谱存在差异,不同菌株间的某些同工酶谱带数差异显著。     

寄主诱导下的玉米弯孢叶斑病菌致病性分化相关蛋白的动态变化研究

 Reinoculation and two-dimensional electrophoresis were performed to analyze the pathogenicity differentiation of Curvularia lunata.Resistant host inbred lines Shen135,Mo17,and 78599-1 were reinoculated(six generations) with low virulent isolate WS18.Results showed that the disease index had no significant change for the first 2 generations of inoculation.At the third generation,the incidence of disease was increased and the number of differential expressed proteins of mycelia were more than that in the first 2 generations.More than 100 differential expressed proteins were found in the mycelia of fifth generation when compared with the original one.In the experiment,10 differentially expressed proteins were identified by MALDI-TOF-MS analysis.Three proteins were related directly to the differentiation of virulence,2 were related to allergen,4 were related to the metabolism of carbon or signaling pathway and 1 was unkonwn to Curvularia lunata.