小麦-滨麦易位系M8657-1抗条锈病基因遗传分析和分子标记

 M8657-1, one of the wheat translocation lines derived from Leymus mollis Trin. Hara, is possessed of effective resistance at all stages to Su-ll and other dominant races of Puccinia striiformis f. sp. tritici in China. Seedlings of the parents, F₁, and F₂ progeny derived from the cross of M8657-1 (resistant) Mingxian169 (susceptible) were inoculated with Su-ll in greenhouse to identify and map the probable new stripe rust resistance gene. The results suggested that the stripe rust resistance in M8657-1 was conferred by a pair of recessive genes. Simple sequence repeat (SSR) technique was used to detect molecular marker associated with the resistance gene:208 pairs of wheat SSR primers were used to screen the two parents, as well as resistant and susceptible bulks and then three SSR markers were selected for genotyping the F₂ population. The geue, temporarily designated as YrLml, was found to be located on the chromosome 7DL and flanked by three SSR markers GDM67, WMC150 and WMC671, with the genetic distance of 5.0, 9.7 and 11.8cM, respectively.     

中国小麦条锈菌鉴别寄主水源11的抗条锈基因

小麦品种水源11是我国小麦条锈菌重要鉴别寄主和抗病种质资源.李剑雁等[1]运用单体分析将其对CYR21、CYR23的抗条锈基因分别定位于3D及5D;刘孝坤[2]研究认为其成株期对CYR25的抗性由1对显性基因控制;杨华安等[3]通过基因推导法推测其至少含有两对抗条锈基因.     

一种快速分离小麦条锈菌转GUS基因转化体的方法

为了获得更多整合有外源GUS报告基因并且稳定表达的小麦条锈菌毒性突变菌株.探索小麦条锈菌夏孢子转GUS基因遗传转化体的分离方法.以从菌种筛选品种上分离转化体法为主、从繁殖品种辉县红上分离转化体法为辅可有效提高转化体的检出率;建立了利用透明胶带粘取夏孢子并通过染色分离小麦条锈菌转GUS基因转化体的新方法.试验证明,透明胶带粘取夏孢子染色法是一种快速有效分离小麦条锈菌转GUS基因转化体的方法.