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1.
T Tsuboi Y Kanazawa T Syoji S Tokuhisa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1179-1181
Bovine follicular oocytes collected from bovine ovaries were exposed to bovid herpesvirus 1 (BHV-1). After washings, these oocytes were cultured to mature. As a result BHV-1 could not be removed from the oocytes and could replicate in the oocytes with cumulus cells, but not in the oocytes without the cells. Moreover, the specific fluorescence for BHV-1 was detected in the cumulus cells by a indirect immunofluorescent technique. Therefore these findings suggested BHV-1 could be absorbed in the oocytes but the replication of BHV-1 was done in the cumulus cells. 相似文献
2.
Mohammad Mijanur Rahman Khairiyah Mat Genki Ishigaki Ryo Akashi 《Animal Science Journal》2021,92(1):e13594
Year by year, huge quantities of by-products are generated during the manufacturing process of soybean-based products. Okara is one of the by-products, and it is an insoluble portion of the soybean. It consists of high moisture (8.4–22.9%); on dry matter basis, it contains high metabolizable energy (9.0–14.2 MJ/kg) and other components that include crude protein (20.9–39.1%), crude fiber (12.2–61.3%), crude fat (4.9–21.5%), and ash (3.4–5.3%). Fermentation of okara improves its nutritional quality and reduces its anti-nutrient contents. Due to animals' palatability, okara can be used to replace the soybean meal/concentrate feed partially or completely in ruminant's diet and partially in nonruminant's diet. Okara feeding does not depress the intake, digestibility, growth, milk production, blood metabolic profiles, and meat quality of animals. However, this by-product decays quickly due to its high moisture content, and its heavy weight and sticky nature make it difficult to process and expensive to dry using conventional methods. This paper thoroughly summarizes the utilization of okara as animal feed in the cause of developing a general guideline with favorable levels of inclusion in the diets of animals for its exploitation and valorization. This review will encourage further research to develop eco-friendly and value added feed for animals. 相似文献
3.
Toshiyuki Usami Shu Ishigaki Hiroko Takashina Yuko Matsubara Yoshimiki Amemiya 《Journal of General Plant Pathology》2007,73(2):89-95
Japanese isolates of Verticillium dahliae, a causal agent of wilt disease in many plants, are classifiable into pathotypes based on their pathogenicity. Because these
pathotypes are morphologically indistinguishable, establishing a rapid identification method is very important for the control
of this pathogen in Japan. For cloning DNA fragments that are useful for identification and specific detection of V. dahliae pathotypes, we performed random amplified polymorphic DNA (RAPD) analyses using various isolates. One polymerase chain reaction
(PCR) product, E10-U48, was specific to isolates pathogenic to sweet pepper. The other product, B68-TV, was specific to race
1 of isolates pathogenic to tomato. The specificity of these sequences was confirmed by genomic Southern hybridization. Further
analyses revealed that the region peripheral to B68-TV obtained from the genomic DNA library includes the sequence specific
to all isolates pathogenic to tomato (races 1 and 2). Moreover, sequence tagged site (STS) primers designed from B68-TV and
its peripheral region showed race-specific and pathotype-specific amplification in a PCR assay. The probes and primers obtained
in this study are likely to be useful tools for the identification and specific detection of pathotypes and races of V. dahliae.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB095266. 相似文献
4.
S. Tokuhisa Y. Inaba K. Sato Y. Miura H. Akashi K. Satoda M. Matumoto 《Veterinary microbiology》1981,6(2):143-155
Eleven, 11 and 2 of 11 precolostral sera from normal calves and 13, 14 and 9 of 14 sera from normal fetuses, 7 to 10 months of gestation, neutralized bovine parvovirus, coronavirus and rotavirus, respectively.When assayed by single radial immunodiffusion, all the sera contained IgG at 360 to 1400 mg/dl, and some of them had much smaller amounts of IgM or IgA. Most of the neutralizing activities against bovine coronavirus and rotavirus were readily inactivated by treatment with acetone or 2-mercaptoethanol. Some sera fractionated by Sephadex G-200 gel filtration or starch block electrophoresis had neutralizing activities against bovine parvovirus or coronavirus in fractions containing no detectable amounts of immunoglobulins. These observations seem to indicate the presence of substance(s), other than immunoglobulins, capable of inhibiting replication of bovine parvovirus, coronavirus or rotavirus. The chemical nature and the mode of action of the inhibitors await elucidation. 相似文献
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6.
Slow-reacting complement-requiring neutralizing (NT) antibody was detected in sera from cattle 2 weeks after infection with Akabane virus. Bovine sera obtained 3 or 4 weeks after infection contained slow-reacting noncomplement-requiring NT antibody. The slow-reacting complement-requiring NT antibody was sensitive to 2-mercaptoethanol (2-ME), whereas the slow-reacting noncomplement-requiring NT antibody was resistant to 2-ME. The initial phase may represent the IgM response and the later phase a change to IgG. A NT test was developed in which virus-serum mixtures were incubated at 4 degrees C for 48 h and then with complement at 37 degrees C for 60 min; this gave an improved sensitivity over the previous incubation at 37 degrees C for 60 min. 相似文献
7.
T Imada H Kawamura T Nishimori H Murata M Narita Y Honda K Ishigaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(1):96-104
The pathogenicity and immunogenicity of vaccinia virus were examined in order to evaluate the possibility of its application as a recombinant viral vaccine in pigs. Following scarification inoculation with vaccinia virus, a mild reddish papulation developed only on the scarified part of the skin. No symptoms of illness such as fever or stunting were noted. Vaccinia virus was recovered in titers from scarified skin 4 and 7 days after inoculation. Control piglets cohabited with inoculated animals remained normal for the whole 5 week observation period. Hemagglutination inhibition and indirect immunofluorescence tests detected antibodies against vaccinia virus in the inoculated piglets, whereas no anti-vaccinia virus antibodies were detected in the contact control animals. Antigen-induced blastogenic tests of peripheral blood lymphocytes from animals, revealed that lymphocytes obtained from inoculated donors 5 weeks after inoculation, had a higher stimulation index (P less than 0.05) than did those from uninoculated piglets. These results suggested that vaccinia virus would be useful as a recombinant viral vector for pigs. 相似文献
8.
H. Akashi Y. Inaba Y. Miura S. Tokuhisa K. Sato K. Satoda 《Veterinary microbiology》1980,5(4):265-276
A coronavirus (Kakegawa isolate) isolated from a cow with epizootic diarrhea was grown in BEK-1 cells and examined for biophysical and biochemical properties. The Kakegawa isolate was able to replicate in the presence or absence of 5-iodo-2′-deoxy-uridine, indicating that its viral nucleic acid was RNA. It was highly sensitive to ether and chloroform, and moderately sensitive to trypsin and heat. It was, however, readily stabilized by treatment with cation at 50°C for 1 h. Its infectivity was slightly reduced at pH 3.0. The virus passed through a membrane filter of 200 nm pore size, but not through one of 100 nm pore size. The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. Neutralization and hemagglutination inhibition tests showed a close serological relationship between the Kakegawa isolate and the American strain of calf diarrhea coronavirus. 相似文献
9.
Sakai M Otani I Ishigaki K Edamura K Takahashi T Koie H Yamaya Y Watari T Shibuya H Sato T Kanayama K Tokuriki M Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1219-1221
We evaluated hepatic T lymphocyte phenotypes in a dog with chronic hepatitis. Before treatment, numerous CD3+ lymphocytes were demonstrated in the liver, and the ratio of CD4+/CD8+ was remarkably high (2.96; reference range, 0.33+/-0.12). After treatment, CD3+ lymphocyte infiltration in the liver was reduced, and the ratio of CD4+/CD8+ decreased to 0.31. Therefore, hepatic T lymphocytes, especially CD4+ lymphocytes, might play a central role in the pathogenesis of this dog with chronic hepatitis. 相似文献
10.
The influence of arbuscular mycorrhizal fungi (AMF) colonization on free amino acid concentrations in strawberry (Fragaria × ananassa Duch., cv.Nohime) plants was investigated using two AMF species [Glomus mosseae (Gm), Gl. aggregatum (Ga)] under phosphorus-supplemented (+P) or non-supplemented (−P) conditions. Ten weeks after AMF inoculation, mycorrhizal plants showed higher values in dry weight of both shoots and roots than did non-mycorrhizal ones among most of the treatments. Shoots and roots of mycorrhizal plants had greater phosphorus concentrations in −P plots, while in +P plots, P concentrations differed little among the inoculation treatments. AMF colonization was greater in plants inoculated with Gm than in plants inoculated with Ga. Total amino acid concentration was higher in most of the plants inoculated with Gm than in non-mycorrhizal ones regardless of P treatment. Serine, glutamic acid, glycine, alanine, leucine and GABA were higher in both mycorrhizal plants in −P plots than in non-inoculated plants. In +P plots, threonine and isoleucine concentrations were greater in both mycorrhizal plants than in non-inoculated ones. Asparagine, glutamic acid, glycine, citrulline, GABA and arginine were greater in plants inoculated with Gm than in non-mycorrhizal ones. These findings verified that inoculation with AMF increases total amino acid concentrations and concentrations of specific amino acids in strawberry plants with or without phosphorus supplementation, though the effects varied with species of mycorrhizal fungus. 相似文献