排序方式: 共有4条查询结果,搜索用时 0 毫秒
1
1.
Gunars Lacis Isaak Rashal Silvija Ruisa Viktor Trajkovski Amy F. Iezzoni 《Scientia Horticulturae》2009
Three previously described highly polymorphic SSR (microsatellite) primer pairs were tested on 126 sweet cherry (Prunus avium L.) accessions to adapt a fast, reliable method for preliminary screening of sweet cherry germplasm collections and to compare two sweet cherry germplasm collections: at the Latvia State Institute of Fruit-Growing, Dobele (LIFG-Dobele) and at the Division of Horticultural Genetics and Plant Breeding at Balsgård, Department of Crop Sciences, Swedish University of Agricultural Sciences (SLU-Balsgård). The SSR loci were highly polymorphic with 4–10 different alleles and 5–18 genotypes. Heterozygosity values ranged from 0.431 to 0.809, gene diversity (PIC) values ranged from 0.400 to 0.753, and the discriminating power of each locus varied from 0.631 to 0.894. The combined discriminating power of all loci was highly effective (0.996). Sixteen identical accession groups with the same allele profile were discovered in both collections. This study demonstrated that SSR fingerprinting with the three primer pairs tested, can be used for preliminary characterization of sweet cherry germplasm collections. 相似文献
2.
Beer-Ljubić B Aladrović J Marenjak TS Majić-Balić I Laškaj R Milinković-Tur S 《Research in veterinary science》2012,92(2):292-294
Bull spermatozoa samples contain variable portion of motile and normal morphology spermatozoa along with spermatozoa incapable of fertilization due to their pathologic changes. As semen quality is influenced by biochemical and morphological characteristics of all spermatozoa, the aim of the study was to separate spermatozoa in discontinuous iodixanol density gradient solution and to determine their cholesterol, phospholipid, triacylglycerol and lipid peroxide concentrations and creatine kinase activity. The study was performed in winter and included seven Simmental bulls aged 1.5-3.5 years. Semen samples were collected by use of artificial vagina. Upon evaluation of semen quality (volume, concentration and progressive sperm motility), the samples were centrifuged in iodixanol density solution to obtain two sperm fractions. The two fractions included sperms with progressive motility greater than 90% and less than 20%, respectively. A statistically significantly higher lipid peroxide concentration was determined in sperm fraction with <20% progressive motility. Different sperm subpopulations can be obtained by separating bull spermatozoa in different iodixanol density gradient solutions, while monitoring their biochemical properties can help assess the sperm quality. 相似文献
3.
Journal of Plant Diseases and Protection - Citrus tristeza virus (CTV) infection in 13 Satsuma (Citrus unshiu (Mak.) Marc.) symptomless clones was investigated by different diagnostic methods,... 相似文献
4.
Silvija Cerni Dijana Skoric Jelena Ruscic Mladen Krajacic Tatjana Papic Khaled Djelouah Gustavo Nolasco 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(4):701-706
Citrus tristeza virus (CTV) represents one of the major threats to citrus production worldwide. In the East Adriatic region, CTV symptoms are mostly
absent due to traditional citrus grafting on trifoliate orange (Poncirus trifoliata), a CTV-tolerant rootstock. Therefore, the virus has been continuously spreading by the propagation of infected material.
The genetic variability of CTV was studied on nineteen citrus samples, collected from orchards in the coastal region of Croatia,
Montenegro and Albania, that previously tested positive by ELISA and immunocapture RT-PCR. Single-strand conformation polymorphism
of the amplified coat protein gene demonstrated the presence of different CTV variants in each amplicon, while sequence analysis
of cloned CP gene variants confirmed their clustering into six out of the seven phylogenetic groups so far delineated. Four
of these groups include sequences of severe quick decline, seedling yellows and stem-pitting (SP) isolates, thought to be
found only rarely in the Mediterranean region. Regardless of the lack of symptoms in the field, CTV isolates from the East
Adriatic displayed high genetic variability and pathogenic potential, additionally confirmed by biological characterisation.
The high percentage of mixed infections suggest the potential for further diversification and a greater risk of severe variants
spreading into new areas. 相似文献
1