Gibberellins: a phytohormonal basis for heterosis in maize

Four commercially important maize parental inbreds and their 12 F(1) hybrids were studied to investigate the role of the phytohormone gibberellin (GA) in the regulation of heterosis (hybrid vigor). All hybrids grew faster than any inbred. In contrast, all inbreds showed a greater promotion of shoot growth after the exogenous application of GA(3). Concentrations of endogenous GA(1), the biological effector for shoot growth in maize, and GA(19), a precursor of GA(1), were measured in apical meristematic shoot cylinders for three of the inbreds and their hybrids by gas chromatography-mass spectrometry with selected ion monitoring; deuterated GAs were used as quantitative internal standards. In 34 of 36 comparisons, hybrids contained higher concentrations of endogenous GAs than their parental inbreds. Preferential growth acceleration of the inbreds by exogenous GA(3) indicates that a deficiency of endogenous GA limits the growth of the inbreds and is thus a cause of inbreeding depression. Conversely, the increased endogenous concentration of GA in the hybrids could provide a phytohormonal basis for heterosis for shoot growth.     

Wetlands, carbon, and climate change

Wetland ecosystems provide an optimum natural environment for the sequestration and long-term storage of carbon dioxide (CO₂) from the atmosphere, yet are natural sources of greenhouse gases emissions, especially methane. We illustrate that most wetlands, when carbon sequestration is compared to methane emissions, do not have 25 times more CO₂ sequestration than methane emissions; therefore, to many landscape managers and non specialists, most wetlands would be considered by some to be sources of climate warming or net radiative forcing. We show by dynamic modeling of carbon flux results from seven detailed studies by us of temperate and tropical wetlands and from 14 other wetland studies by others that methane emissions become unimportant within 300 years compared to carbon sequestration in wetlands. Within that time frame or less, most wetlands become both net carbon and radiative sinks. Furthermore, we estimate that the world’s wetlands, despite being only about 5–8 % of the terrestrial landscape, may currently be net carbon sinks of about 830 Tg/year of carbon with an average of 118 g-C m^?2 year^?1 of net carbon retention. Most of that carbon retention occurs in tropical/subtropical wetlands. We demonstrate that almost all wetlands are net radiative sinks when balancing carbon sequestration and methane emissions and conclude that wetlands can be created and restored to provide C sequestration and other ecosystem services without great concern of creating net radiative sources on the climate due to methane emissions.     

Effects of land use intensity on soil nutrient distribution after reclamation in an estuary landscape

The effects of time on the evolution of land use intensity and soil nutrients distribution were studied in a reclamation zone of the Yangtze Estuary. Land use types were grouped into five intensity levels according to the extent of human disturbance. We used the “space for time substitution” method to test the impact of time on changes in land use intensity after reclamation and found that land use levels increased quickly within the first 35 years, then slowed. Soil salinity was relatively higher in aquaculture ponds than that in areas with other types of land cover due to the use of saline water from underground and the sea. Soil organic matter, available phosphorous and nitrate nitrogen were relatively high in agricultural fields, while nitrate nitrogen was highly variable in agricultural fields. The variations of all four soil properties in the built-up zone were much higher than those in the other land use groups. The spatial distribution of different nutrients is the combined effect of time and land use post reclamation. The results will provide a sound basis for future land use planning of newly reclaimed land, and for further studies on ecological consequences of salt marsh reclamation.     

Regulation of gibberellin biosynthesis and stem elongation by low temperature in Eustoma grandiflorum

Summary

Heat-induced rosetted Eustoma grandiflorum requires low temperature for induction of stem elongation and flowering. Although heat-induced rosetting is associated with a reduction of gibberellin A₁ (GA₁) content, how thermo-induction affects GA biosynthesis is unclear. Thus, we examined levels of GA, precursors including that of ent-kaurene which is the first committed step in GA biosynthesis. We used uniconazole, an ent-kaurene oxidase inhibitor to estimate the ent-kaurene biosynthesis activity. The accumulation level of ent-kaurene in stems of the cold-treated seedlings was approximately 1.8 times that of the non-cold-treated seedlings, whereas no difference was observed in the leaves. No change was observed in endogenous levels of GA₁ and GA₂₀ in stems of the heat-induced rosetted plants during the cold treatment, whereas their levels increased with stem elongation after transfer to warm conditions. In contrast to the levels of GA₁ and GA₂₀, endogenous levels of ent-kaurene, ent-kaurenoic acid, GA₅₃, GA₄₄ and GA₁₉ in the stems markedly increased at the end of cold treatment. These results indicate that ent-kaurene biosynthesis and its metabolism early in the GA biosynthetic pathway are stimulated by low temperature and, later, the stimulation leads to an increment of endogenous levels of GA₁ which is essential for stem elongation of the heat-induced rosetted E. grandiflorum.     

Nutrient flows and land use change in a rural catchment: a modelling approach

Due largely to unprecedented land-use changes in the Porijõgi River catchment (southern Estonia) losses of nutrients and organic matter have decreased significantly. During the period 1987–1997 abandoned lands increased from 1.7 to 10.5% and arable lands decreased from 41.8 to 23.9%. At the same time, the runoff of total-N, total-P, SO₄ and organic matter (after BOD₅) decreased from 25.9 to 5.1, 0.32 to 0.13, 78 to 48, and 7.4 to 3.5 kg ha^–1 yr^–1, respectively. The most significant decreases occurred in agricultural subcatchments while the changes were insignificant in the forested upper course catchment. A simple empirical model which incorporates land-use pattern, fertilization intensity, soil parameters and water discharge accurately described the variations of total-N and total-P runoff in both the whole catchment and its agricultural subcatchments (R ² varies from 0.95–0.99 for N to 0.49–0.93 for P). In small agricultural subcatchments the rate of fertilization is found the most important factor for nitrogen runoff, whereas in larger mosaic watersheds land use pattern plays the main role. Seven alternative scenarios compiled on the base of the empirical model allow to forecast potential nitrogen and phosphorus losses from the catchment. This information can be used in further landscape and regional planning of the whole region.     

Nutrient flows and management of a small watershed

Nutrient leaching from agricultural areas is one of the main concerns of watershed management. The paper examines nitrogen and phosphorus leaching from different parts of small agricultural watershed (378 ha) that was divided into 6 subcatchments. The calculations of nutrient outflow are based on the detailed measurement at the time of intensive agricultural activities during 5 years (1987–1991). The results show that nutrient leaching can vary very much even in such a small catchment area. The retention of nitrogen and phosphorus took place in the storage lake: 3,900 and 2.2 kg ha⁻¹ year⁻¹, respectively. At the same time, in the small subcatchment with high shallow groundwater outflow value, the nitrogen and phosphorus outflow was 233 and 0.90 kg ha⁻¹ year⁻¹, respectively. The most effective mitigation method is establishing buffer zones on the banks of the stream. A buffer zone of 460 m length would remove 2,200 to 2,640 kg N and 12 to 15 kg P a year, a constructed wetland on the stream would remove 1,660 to 2,760 kg N and 3 to 4.5 kg P a year. The detailed study gives good opportunity to estimate most critical areas where application of mitigation methods is most needed and ecologically and economically effective.

     

Incidence and diversity of phosphate-solubilising bacteria are linked to phosphorus status in grassland soils

The extent to which soil phosphorus (P) status affected the incidence of soil phosphate-solubilising bacteria (PSB) and their taxonomic abundance and diversity was examined at three long-term fertiliser trials (Whatawhata, Winchmore and Ballantrae) in New Zealand. Bacteria were isolated from rhizosphere (ryegrass and clover) and non-rhizosphere soils differing in P status. The P-solubilising phenotype was determined on agar supplemented with sparingly-soluble mineral phosphates (Ca₂OH(PO₄)₃ and CaHPO₄). The frequency of P-solubilisation in the bacterial population was significantly greater (P < 0.001) in soils of low-P status, demonstrating a selection pressure for this trait based on soil P availability. P-solubilising bacteria from high-P level soils and soils which had not received P fertiliser (nil-P soils) were identified based on 16S rRNA-gene sequence analysis. Across the samples, the P-solubilising community was very rich with 39 genera of PSB found, spanning 24 families and 4 phyla. At Ballantrae and Winchmore, the PSB composition differed (P < 0.05) across soil P status, which was associated with an alteration in abundance of Actinobacteria, Pseudomonadaceae and Moraxellaceae. The phylogenetic composition of PSB differed significantly (P < 0.05) between sites, however nearly half the families were common across all sites, constituting a ‘core community’ of P-solubilising bacteria for these New Zealand pasture soils. As the abundance and composition of P-solubilising bacteria are under strong selection pressure affected through farm management strategies, better understanding of their ecology provides the opportunity to increase the availability of soil P for plant-uptake.     

Greenhouse gas fluxes in an open air humidity manipulation experiment

Air moisture is one of the main factors causing the greenhouse effect, but humidity has not been thoroughly studied at the ecosystem level. In 2006 a free-air humidity manipulation (FAHM) facility was established in Estonia to investigate the effect of humidity on the performance of two tree species—silver birch (Betula pendula Roth.) and hybrid aspen (Populus tremula L. × P. tremuloides Michx.). The trial is located on an Endogleyic Planosol of former arable land and offers the opportunity to change relative air humidity through controlled artificial misting and drying. We measured the CO₂, CH₄ and N₂O emission from the FAHM system using closed chamber and gas-chromatograph techniques from July 2009 to October 2010. Measurements were carried out once a month in three humidification (h) plots and in three control (c) plots. The vegetation period was rainy in 2009, but the next summer was relatively dry. The dry summer interacted better with the humidification. The CO₂ flux decreased when the air moisture was higher than in the control plots. The soil acted as a sink for methane. Less CH₄ was oxidized in the soil with increased humidity. Emission of N₂O did not correlate with air humidity, although one could observe a clear tendency that there was a smaller N₂O flux during the period with increased humidity. Expectedly, CO₂ emission and CH₄ consumption demonstrated strong positive correlations with soil temperature.     

Tumour angiogenesis,anti‐angiogenic therapy and chemotherapeutic resistance

In order for a tumour to continue to grow and disseminate, it must acquire a new blood supply. Neovascularisation can be enacted by a number of different mechanisms. This dependence of tumour progression on an augmented vascular supply has been exploited by the development of anti‐angiogenic drugs, which are designed to inhibit new blood vessel formation or disrupt existing tumour‐associated vasculature, both leading to ischaemic–hypoxic tumour cell death. However, the clinical benefits of these therapeutic approaches are frequently variable and often transient, the neoplasm sometimes being able to use other neovascularisation mechanisms to maintain its blood supply and thus evade the current anti‐angiogenic therapy. Tumours may also develop a more malignant phenotype following this treatment. Clinical outcomes may be improved by simultaneously inhibiting different angiogenic pathways, abetted by more effective drug delivery regimens such as metronomic chemotherapy and the concurrent use of other antitumour modalities.