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1.
Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 相似文献
2.
Hiromitsu Furuya Tsutomu Matsumoto Shin-ichi Fuji Hideki Naito 《Journal of General Plant Pathology》2003,69(2):115-119
Rice seedling growth, estimated by plant height and root development and discoloration, was better in pasteurized soil than
in unpasteurized soil obtained from a flooded rice field. Rice seedlings also grew better in sterilized soil modified by adding
roots harvested from the pasteurized soil than in soil modified by adding roots harvested from the unpasteurized soil. The
results demonstrate that seedling growth in the rice field soil was inhibited by soil microorganisms, even though no typical
symptoms such as seedling blight or damping-off appeared. Pythium aristosporum is suggested to be involved in the inhibition. Thus, it appears that inconspicuous restraint of rice seedling growth could
occur in soils of rice paddy fields.
Received: May 20, 2002 / Accepted: October 16, 2002
Acknowledgments The authors thank Dr. T. Ichitani, former professor at Osaka Prefectural University, for providing an isolate of Pythium aristosporum for comparison, and Mr. Mitsuaki Sato of Akita Prefectural College of Agriculture for technical assistance. 相似文献
3.
Evaluation of high sensitive DNA probe for the detection of Theileria sergenti infection in cattle. 总被引:2,自引:0,他引:2
4.
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6.
Hasegawa D Fujita M Nakamura S Takahashi K Orima H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(3):277-279
A Shetland sheepdog with epilepsy refractory to antiepileptic drugs was brought to the division of Veterinary Radiology at Nippon Veterinary and Animal Science University. Scalp electroencephalography and computed tomography was performed, but no abnormality was detected in either examination. To obtain detailed information, electrodes were implanted on the dura mater, and the electrocorticogram (ECoG) was recorded. In the ECoG, sporadic spikes were detected in the left parietal region, suggesting the presence of the epileptic focus in this region. After the dog's death, abnormalities of gyri were found in the region where spikes were detected in the ECoG. On histopathological examination, laminar malacia of the cingulate gyrus was observed. Furthermore, in the hippocampus, neuronal loss of pyramidal cells was observed. 相似文献
7.
Komatsu Y Itoh N Taniyama H Kitazawa T Yokota H Koiwa M Ohtsuka H Terasaki N Maeno K Mizoguchi M Takeuchi Y Tanigawa M Nakamura T Watanabe H Matsuguchi Y Kukino T Honma A 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2002,49(9):482-486
Histopathological features of livers and blood chemical values in cows with abomasal displacement were investigated. Liver biopsy samples were collected during redressment operations in 92 cows with abomasal displacement, and the samples were stained with haematoxylin and eosin or periodic acid Schiff (PAS). Blood was collected for chemical tests. Livers were histopathologically divided into the following four types: normal histology cases (21%), fatty degeneration cases (36%), cloudy swelling cases (19%) and fatty degeneration cases with cloudy swelling (24%). The number of PAS-positive samples was significantly higher in the normal histology group and significantly lower in the severe fatty degeneration group and severe cloudy swelling group. Cows with fatty degeneration had significantly higher levels of serum 3-hydroxybutyric acid, non-esterified fatty acid and aspartate aminotransferase than did those with cloudy swelling or normal histology. The results indicate that the morbid conditions of cows with abomasal displacement can be classified into four types. 相似文献
8.
Hara Y Nezu Y Harada Y Hasegawa D Fujita M Orima H Tagawa M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(9):863-866
An intradural tumor in the upper cervical region was found in a dog with quadriparesis and chronic respiratory acidosis. Surgical removal of the tumor in the atlas and intraoperative radiotherapy were attempted. The tumor was histologically diagnosed as a neural glioma. A preoperative acid-base disturbance was dramatically improved after surgery. The clinical changes appeared in this case suggest that compression of the spinal cord at this region may cause paralysis of the respiratory muscles and secondarily result in chronic respiratory acidosis following the respiratory insufficiency. 相似文献
9.
In vitro detection of equine arteritis virus from seminal plasma for identification of carrier stallions 总被引:1,自引:0,他引:1
Fukunaga Y Wada R Sugita S Fujita Y Nambo Y Imagawa H Kanemaru T Kamada M Komatsu N Akashi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(6):643-646
Equine arteritis virus (EAV) was readily isolated in RK-13 cell monolayers by plaque assay from seminal plasma of experimental carrier stallions when they contained high titers of virus regardless of the presence of non-viral cytotoxicity in the seminal plasma. The cytotoxicity interfered with virus isolation from seminal plasma which contained virus at titers less than 10 PFU/ml. However, it was possible to detect the virus in seminal plasma pretreated with PEG (#6000). EAV was consistently identified by RT-PCR from crude seminal plasma which contained virus at titers of more than 10(2.7) PFU/ml. In vitro detection of EAV by virus isolation supplemented with RT-PCR using seminal plasma was proved to be an effective alternative to the standard test mating as a diagnostic method for carrier stallions. 相似文献
10.
In Japan, the lifetime cycle of most housing lasts around 20–30 years. A governing factor in this respect is poor durability
due to old-fashioned use of the house. As a solution of this problem, houses can be built with a skeleton structure that allows
free partition of spaces by future owners. To develop the skeleton structure effectively, multistory frames with spans of
6 to 10 m are required. For this reason, attention has been focused on the behavior of multistory timber frame structures.
In this article, two types of wooden portal frame structures are proposed. Both structures have improved vertical columns
with short horizontal members glued in. The aim of this study was to investigate structurally effective solutions with these
types of columns. The first type of the new structure changed the location of the moment-transmitting ductile connection with
the improved columns. The second type of structure used an extended panel zone. Nine portal frame specimens were tested. The
stiffness values were improved by around 1.7 and 3.5 times when compared with the control, and the strength was improved by
around 1.25 and 1.45 times. 相似文献