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排序方式: 共有288条查询结果,搜索用时 46 毫秒
1.
Influence of death struggle on the structural changes in chub mackerel muscle during chilled storage
Masashi Ando Masao Joka Satoshi Mochizuki Koh-Ichi Satoh Yasuyuki Tsukamasa Yasuo Makinodan 《Fisheries Science》2001,67(4):744-751
ABSTRACT: Chub mackerel (34–35 cm, approximately 500 g), which were caught by fishing with a rod and line at the Bungo Channel, Oita prefecture, were rested overnight in a fish preserve and either killed by decapitation (control group) or allowed to struggle in air for 30 min (struggled group). Muscle samples were excised every 4 h, and measurements on breaking strength and histological observations were done for both groups. The breaking strength of muscle in the control group was significantly higher than that in the struggled group, whereby a decrease in breaking strength was delayed for 12 h compared to the struggled group. Light microscopy showed space extension among muscle cells in association with a decrease in breaking strength. Especially in the struggled group, the extended area was larger and the difference in area was significant at the time when breaking strength showed a significant difference. Using electron microscopy, the extended area showed cut and/or disappeared collagen fibrils. From these results, it was demonstrated that struggling to death promoted the degradation of collagen fibrils and the weakening of connective tissue and, resultantly, led to the faster softening of muscle of chub mackerel. 相似文献
2.
Hitomi NAKABAYASHI Yasuyuki YAMAJI Satoshi KAGIWADA Masashi UGAKI Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(2):173-176
The complete nucleotide sequence was determined for genomic RNA of White clover mosaic virus (WClMV-RC) isolated from red clover (Trifolium pratense) in Japan, It is 5843 nucleotides in length, excluding the poly(A) tail at the 3' terminus. Similar to other potexviruses,
it contains five open reading frames (ORFs 1 through 5), which putatively encode an RNA-dependent RNA polymerase (RdRp) (147
kDa), a triple gene block (TGB) (26 kDa/13 kDa/7 kDa), and a coat protein (CP) (22 kDa), respectively. The deduced amino acid
sequence of the WClMV-RC CP was identical to that of WClMV-O, one of two New Zealand isolates, but only 85% identical to that
of WClMV-M, the other New Zealand isolate, because of heterogeneity in the C-termini of CP amino acid sequences. The implication
of this CP heterogeneity is discussed.
Received 30 August 2001/ Accepted in revised form 11 January 2002 相似文献
3.
Ayano OMURA Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):43-47
The digestive organs in decapodiform cephalopod species morphologically vary by
individual lifestyle. We examined the following six species of adult decapodiformes
cephalopods representing different habitats: Todarodes pacificus, Loligo
bleekeri, Loligo edulis, Watasenia
scintillans (pelagic), Sepia lycidas and Euprymna
morsei (benthic). L. bleekeri and L. edulis
possess a bursiform cecal sac connected to the cecum. Pelagic species have a single
digestive gland smaller than in benthic species. T. pacificus has an oval
digestive gland larger than that of L. bleekeri and L.
edulis, which possess withered-looking and smaller digestive glands. In
contrast, the digestive glands in benthic species are paired. S. lycidas
and E. morsei have well-developed and larger digestive glands than those
of the pelagic species. Well-developed digestive duct appendages are found in benthic
species. In qualification of the mass of digestive organs, pelagic species have smaller
stomachs, digestive glands and digestive ducts’ appendages than benthic species. Because
pelagic species need to swim, they may possess smaller stomachs and larger cecums for more
rapid digestion. A smaller digestive gland may have the advantage of reducing the body
weight in pelagic species for rapid swimming. In contrast, since benthic species require a
longer time for digestion than pelagic species, they compact more food in their stomachs
and absorb nutrients via more organs, such as the digestive grand and digestive duct
appendages, in addition to cecum. 相似文献
4.
Chiho KANEKO Yasuyuki KANEKO Putu Eka SUDARYATMA Hirohisa MEKATA Yumi KIRINO Ryoji YAMAGUCHI Tamaki OKABAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):680
We isolated two pseudorabies virus (PRV) isolates (designated OT-1 and OT-2) from two hunting dogs exhibiting neurological manifestations after eating the flesh of wild boar hunted in Oita prefecture, Kyushu Island, Japan. The isolates corresponded to a previously reported PRV (MY-1 strain) isolated from a hunting dog in neighboring Miyazaki prefecture, and it clustered into genotype II based on the glycoprotein C sequence. Our results suggest that this common PRV strain may have been maintained in wild boars on Kyushu Island even though domestic pigs in this area have attained an Aujeszky’s disease-free status. 相似文献
5.
Son Truong NGUYEN Masaharu MOTOKAWA Tatsuo OSHIDA Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):187-198
Southeast Asia is a region of high biodiversity, containing species of plants and animals that are yet to be
discovered. In this region, bats of the subfamily Kerivoulinae are widespread and diverse with six species
recorded in Vietnam. However, the taxonomy of the Kerivoulinae in Asia is complicated. In our study, we used
diagnostic characters and multivariate analysis to determine morphological differences between the genera
Kerivoula and Phoniscus. We showed that the two genera are distinguishable
by the size of second upper incisors, the shape of skull, nasal sinus, canines, second upper and lower
premolars. In addition, the two genera can be osteometrically separated by measurements of the braincase
height, interorbital width and shape of anterior palatal emargination. Our data clearly revealed the
morphological variations in the skull shape of Kerivoula hardwickii in Vietnam. This suggests
a possible separation into three morphotypes, representing cryptic species supported by statistical
differences with wide variation in skull shape, size and teeth. These results demonstrated Kerivoula
hardwickii can be separated three subspecies, and the result will serve as the basis for the future
assessment and classification of this group in Southeast Asia. 相似文献
6.
Natsumi ENDO Larasati Puji RAHAYU Toshiya ARAKAWA Tomomi TANAKA 《The Journal of reproduction and development》2016,62(1):115-119
Here, we report a new method for measuring behavioral patterns during estrus in goats based on video
tracking analysis. Data were collected from cycling goats, which were in estrus (n = 8) or not in estrus (n =
8). An observation pen (2.5 m × 2.5 m) was set up in the corner of the female paddock with one side adjacent
to a male paddock. The positions and movements of goats were tracked every 0.5 sec for 10 min by using a video
tracking software, and the trajectory data were used for the analysis. There were no significant differences
in the durations of standing and walking or the total length of movement. However, the number of approaches to
a male and the duration of staying near the male were higher in goats in estrus than in goats not in estrus.
The proposed evaluation method may be suitable for detailed monitoring of behavioral changes during estrus in
goats. 相似文献
7.
The aims of this study were to develop a new real-time quantitative PCR (QPCR) assay based on IS900 for detection and quantification of Mycobacterium avium subsp. paratuberculosis (MAP) DNA in faeces, and to use this to detect infected sheep. Both the C and S strains of MAP were detected by the QPCR assay, and no cross reactions were detected with 51 other species of mycobacteria including 10 which contained IS900-like sequences. One copy of IS900 fragment cloned into plasmid pCR2.1 and 1 fg of MAP genomic DNA were consistently detected, while in spiked faecal samples the detection limit was 10 viable MAP per gram of ovine faeces. A total of 506 individual ovine faecal samples and 27 pooled ovine faecal samples with known culture results were tested. The QPCR assay detected 68 of 69 BACTEC culture positive individual faeces and there was a strong relation between time to detection in culture and DNA quantity measured by QPCR (r= -0.70). In pooled faecal samples, QPCR also agreed with culture (kappa=0.59). MAP DNA was detected from some culture negative faecal samples from sheep exposed to MAP, suggesting that the QPCR has very high analytical sensitivity for MAP in faecal samples and detects non-viable MAP in ovine faeces. None of the faecal samples from 176 sheep that were not exposed to MAP were positive in QPCR. This is the first report of a direct faecal QPCR assay that has similar sensitivity to a gold standard radiometric culture assay. 相似文献
8.
Kobayashi T Momoi Y Iwasaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):887-892
The effects of the calcineurin inhibitors cyclosporine A (CsA) and FK506 on the mRNA expressions of various cytokines were evaluated in dogs to determine whether the effects of CsA and FK506 in dogs were similar to those in humans. The mRNA expression levels of the cytokines IL-2, IL-4, IFN-gamma and TNF-alpha were measured in PHA-stimulated canine PBMC using real-time RT-PCR after incubation with CsA or FK506 for 5 hr. Both reagents inhibited IL-2, IL-4 and IFN-gamma mRNA expressions in a dose-dependent manner. However, CsA hardly inhibited the mRNA expression of TNF-alpha. These findings are important for assessing the indications of CsA treatment in dogs. 相似文献
9.
Seiji TSUGE Ayako FURUTANI Rie FUKUNAKA Yasuyuki KUBO Osamu HORINO 《Journal of General Plant Pathology》2001,67(1):51-57
Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae) T7174 is virulent on rice cultivar IR24 and avirulent on IR-BB2. From recent reports, some virulence and avirulence factors
of plant pathogenic bacteria are transferred to plant cells through the hrp-dependent type III secretion system. In this study, we investigated the involvement of hrp genes in the compatible and the incompatible interactions between rice and X. o. pv. oryzae after co-inoculation with hrpXo mutants derived from T7174 and virulent strains. Growth of the mutants, named 74ΔHrpXo and 76ΔHrpXo, was repressed in IR24 when the mutants were applied alone. However, growth of the mutants was complemented by co-inoculation
with virulent strains. Growth of bioluminescent hrpXo mutant 76ΔHrpXo in IR24 and its growth in IR-BB2 after co-inoculation with T7133, which is virulent on both cultivars, was equally complemented,
as detected by bioluminescence from the mutant. On the other hand, only partial complementation of growth of T7174L76, which
is a bioluminescent and pathogenic derivative of T7174, by T7133 was observed in IR-BB2. Thus, growth of the hrpXo mutant of X. o. pv. oryzae was complemented by virulent strains in both susceptible and resistant rice leaves with the parental strain.
Received 21 July 2000/ Accepted in revised form 26 October 2000 相似文献
10.
Yoshida H Momoi Y Taga N Ide K Yamazoe K Iwasaki T Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):663-669
Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 x 10(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs. 相似文献