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1.
Haitham Sayed Gunter Backes Hamed Kayyal Amor Yahyaoui Salvatore Ceccarelli Stefania Grando Ahmad Jahoor Michael Baum 《Euphytica》2004,135(2):225-228
A partial genetic linkage map was constructed on 71 doubled-haploid lines derived from a cross between the barley lines Tadmor
and WI2291 with 181 molecular markers. The segregating population was used to detect markers linked to the gene Mlg conferring resistance to powdery mildew (Erysiphe graminis f. sp. hordei) and to genes for quantitative resistance to scald (Rhynchosporium secalis). The gene Mlg on chromosome 4H was flanked by two AFLP markers at a distance of 2.0 and 2.4 cM, respectively. QTLs for resistance to scald
were detected on chromosomes 2H and 3H. This association of molecular markers with qualitative and quantitative disease resistance
loci represents a valuable starting-point for marker-assisted selection.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
2.
Sameh Boukef Bruce A. McDonald Amor Yahyaoui Salah Rezgui Patrick C. Brunner 《European journal of plant pathology / European Foundation for Plant Pathology》2012,132(1):111-122
The occurrence of fungicide resistance in Mycosphaerella graminicola populations from Tunisia was investigated by examining mutations known to be associated with strobilurin and azole resistance.
Few mutations associated with fungicide resistance were detected. No evidence for strobilurin resistance was found among 357
Tunisian isolates and only two among 80 sequenced isolates carried mutations associated with azole resistance. A network analysis
suggested that these mutations emerged independently from mutations found in previously described European populations. The
population genetic structure of M. graminicola in Tunisia was analyzed using variation at 11 microsatellite loci. Populations in Tunisia were characterized by high gene
and genotype diversity. All populations were in gametic equilibrium and mating type proportions did not deviate from the 1:1
ratio expected under random mating, consistent with regular cycles of sexual reproduction. In combination with a high degree
of gene flow among sampling sites, M. graminicola must be considered a pathogens with high evolutionary potential. Thus, control strategies against Septoria blotch in Tunisia
should be optimized to reduce the emergence and spread of resistant isolates. 相似文献
3.
Mathew M. Abang Bassam Bayaa Barakat Abu-Irmaileh Amor Yahyaoui 《Crop Protection》2007,26(12):1723-1732
Broomrapes (Orobanche spp.) are aggressive and damaging parasitic weeds which have a tremendous impact on agriculture in East Africa, the Mediterranean region and the Middle East. Despite the availability of technologies to control broomrapes in economically important crops, Orobanche infestation continues to increase, threatening the livelihoods of millions of farmers. Many of the technologies developed have not been effectively disseminated and there has been little or zero adoption by farmers—who continue to use ineffective management practices that exacerbate the problem. The adaptation and dissemination of appropriate management practices are major priorities in broomrape control. However, such work must take into consideration the specific socio-economic characteristics of individual farming systems. Orobanche is a community threat and effective management requires a community-based integrated management approach. Recognizing the central role of farmers in parasitic weed management, a technical cooperation project (TCP) involving FAO, ICARDA and seven countries in the Near East and North Africa (NENA) region was implemented to improve the dissemination of knowledge and skills by using a farmer field school approach: a form of education that uses experiential learning methods to build farmers’ expertise. This paper reviews conventional Orobanche research and development approaches, and highlights weaknesses in the management of the parasitic weed using these approaches as opposed to participatory approaches. The benefits and challenges of participatory farming system approaches in relation to integrated broomrape management (IBM) are also discussed. Lessons learned from achieving community ownership of, and institutional support for, IBM could be applied to other sectors (e.g. public health) in which there is a need for institutional learning and reform. Recommendations are made that include regional collaboration within the framework of a proposed Near East and North Africa Orobanche Management Network (NENAOMAN). 相似文献
4.
ABSTRACT Pyrenophora tritici-repentis, causal agent of tan spot, induces necrosis and chlorosis in its wheat host. The tan spot system conforms to the toxin model and three host-specific toxins have been identified (Ptr ToxA, Ptr ToxB, and putative Ptr ToxC). Processing of a collection of isolates, obtained in the Fertile Crescent and Caucasus regions, yielded two new virulence patterns. Isolate Az35-5 combined the virulences of races 2 and 5 and was classified in the new race 7. Isolates TS93-71B and TS93-71F had a virulence pattern that combined those of races 2, 3, and 5 and were grouped in the new race 8. Southern analysis revealed that all three isolates possessed copies of the ToxA and ToxB genes, the first time the genes were found in a common background. The production of Ptr ToxA and Ptr ToxB by the isolates was confirmed by western blotting. Virulence patterns suggested that TS93-71B and TS93-71F may also produce Ptr ToxC, even though it was not present at detectable levels in culture filtrates. The identification of races 7 and 8 complete the theoretical maximum number of races that can be differentiated by three loci in the host (2(3) = 8), assuming a one-to-one relationship. It appears that the wheat/P. tritici-repentis system is a mirror image of the classical gene-for-gene relationship. 相似文献
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6.
Sarrah Ben M'Barek Petteri Karisto Wided Abdedayem Marwa Laribi Moez Fakhfakh Hajer Kouki Alexey Mikaberidze Amor Yahyaoui 《Plant pathology》2020,69(9):1655-1665
Mixtures of cultivars with contrasting levels of resistance can suppress infectious diseases in wheat, as demonstrated in numerous field experiments. Most studies focus on airborne pathogens in bread wheat, while splash-dispersed pathogens have received less attention, and no studies have been conducted in durum wheat. We conducted a 2-year field experiment in Tunisia to evaluate the performance of cultivar mixtures with varying proportions of resistance (0%–100%) in controlling the polycyclic, splash-dispersed disease septoria tritici blotch (STB) in durum wheat. To measure STB severity, we used a high-throughput method based on digital image analysis of 3,074 infected leaves collected from 42 and 40 experimental plots during the first and second years, respectively. This allowed us to quantify pathogen reproduction on wheat leaves and to acquire a large data set that exceeds previous studies with respect to accuracy and precision. Our analyses show that introducing only 25% of a disease-resistant cultivar into a pure stand of a susceptible cultivar provides a substantial reduction of almost 50% in disease severity compared to the susceptible pure stand. However, incorporating two resistant cultivars instead of one did not further improve disease control, contrary to predictions of epidemiological theory. Susceptible cultivars can be agronomically superior to resistant cultivars or be better accepted by growers for other reasons. Hence, if mixtures with only a moderate proportion of the resistant cultivar provide a similar degree of disease control as resistant pure stands, as our analysis indicates, such mixtures are more likely to be accepted by growers. 相似文献
7.
Gunter Backes Jihad Orabi Asmelash Wolday Amor Yahyaoui Ahmed Jahoor 《Genetic Resources and Crop Evolution》2009,56(1):85-97
Remarkable morphological variation has been found within small Eritrean barley fields. Barley was collected from fields approximately
50 m2 in size. Spike shape, type, and colour were observed to vary both between and within fields. A set of 39 Simple Sequence
Repeats (SSR) markers were used to explore the genetic diversity of the Eritrean barley collected from small-scale farmer’s
fields. Significant genetic diversity was found within the barley fields. Out of 240 spikes collected from 24 fields (10 spikes
per field), only two spikes from geographically distant fields were genetically similar. Based on the SSR data, individual
farmers’ fields were found to possess 97.3% of the genetic variation present in the Eritrean barley. We discuss a strategy
to improve the barley yield in Eritrea, and to facilitate the in situ conservation of barley genetic diversity.
Gunter Backes and Jihad Orabi contributed equally to this work. 相似文献
8.
Genetic and Phenotypic Diversity in the Graminaceous Cyst Nematode Complex, Inferred from PCR-RFLP of Ribosomal DNA and Morphometric Analysis 总被引:3,自引:0,他引:3
Roger Rivoal Sylvie Valette Sadia Bekal Jean-Pierre Gauthier Amor Yahyaoui 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(3):227-241
Graminaceous cyst nematodes form a group of eleven valid species including Heterodera avenae, Heterodera filipjevi and Heterodera latipons and constitute major pests to cereals. They are widely spread in circum-mediterranean areas where they are presumed to cause yield losses on bread and durum wheat. The objective was to document the diversity of these cereal cyst nematodes, in particular samples from Mediterranean regions, in comparison to species which develop on cultivated or wild grasses (H. arenaria, H. hordecalis, H. mani) and on rice or sugarcane (H. sacchari). Studies involved PCR-RFLP of ITS and morphometrics of the juvenile and cyst characters. UPGMA analysis of molecular data showed that the isolates segregated in two main clusters which seem to represent different evolutionary lineages. The H. avenae sensu lato cluster (I) contained H. arenaria, H. avenae, H. filipjevi and H. mani. The second cluster (II) contained isolates of H. hordecalis and H. latipons. Within H. avenae sensu lato, H. filipjevi was separated from the other related species with significant bootstrap value. The differentiation of H. arenaria, recognized for the first time based on molecular data, and H. mani with few restriction enzymes were the least significant. Intraspecific polymorphism allowed differentiation of isolates originating from Australia within H. avenae sensu stricto. The group H. hordecalis–H. latipons showed the greatest genetic variability between and within isolates. Two representatives of Heterodera sacchari, taxonomically included in the schachtiigrave group, were genetically as distant to this group as to the other graminaceous species belonging to either H. avenae sensu lato or H. hordecalis–H. latipons group. Results inferred from multivariate analysis applied on morphometrics of the cysts and juveniles showed agreement between genetic and phenotypic classifications. This study demonstrates the utility of combined molecular and classical methods to enhance our knowledge about the diversity within the complex of graminaceous cyst nematodes and to establish robust techniques to identify a wider set of nematode species. 相似文献
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10.
Abang MM Baum M Ceccarelli S Grando S Linde CC Yahyaoui A Zhan J McDonald BA 《Phytopathology》2006,96(11):1214-1222
ABSTRACT Competition among eight Rhynchosporium secalis isolates was assessed during parasitic and saprophytic phases of the disease cycle in field experiments conducted at two locations and over two growing seasons. The eight isolates were inoculated onto six barley populations exhibiting varying degrees of resistance. Microsatellite analysis of 2,866 isolates recovered from the field experiments showed significant, and sometimes opposite, changes in the frequencies of R. secalis genotypes during the growing season (parasitic phase) and between growing seasons (saprophytic phase). Isolates that showed the most complex virulence in greenhouse seedling assays had the lowest fitness in the field experiment. Significant differences in isolate fitness were found on different host populations and in different environments. Selection coefficients were large, indicating that evolution can occur rapidly in field populations. Although inoculated isolates had the lowest overall fitness on the moderately resistant landrace cv. Arabi Aswad, some isolates were more virulent and consistently increased in frequency on this landrace, suggesting a risk of directional selection and possible erosion of the resistance following its widespread deployment in monoculture. These results provide the first direct evidence that R. secalis pathogen genotypes differ in their saprophytic ability and parasitic fitness under field conditions. 相似文献