Reproductive performance in a diallel cross among lines of mice selected for litter size and body weight

Genetic factors affecting female reproductive performance in lines of mice with a known history of selection were estimated from a 5 X 5 diallel cross. Lines were selected as follows: large litter size at birth (L+); large 6-wk body weight (W+); an index for large litter size and small 6-wk body weight (L+W-); the complementary index (L-W+) and randomly (K). Partitioning of direct and correlated responses for litter size, 6-wk body weight and related traits into average direct genetic (li) and average maternal genetic (mi) effects indicated that the magnitude of differences in li exceeded those in mi. Lines having positive responses in li were W+ greater than L+ greater than L-W+ for dam body weight, L+ greater than L+W- greater than W+ for litter size and L+ greater than (W+, L+W-) for litter birth weight, whereas L-W+ responded negatively for litter size. A positive association was found between mi for litter size and dam body weight, W+ and L-W+ being high and L+ and L+W- low for both traits. Female infertility and time from male exposure to parturition had relatively small correlated responses. Line rankings in general combining ability (gi) and net line effects were similar for the respective traits. Depending upon the line and trait involved, the relative contribution of average direct genetic and line direct heterotic (hi) effects to general combining ability [gi = (1/2) li + hi] varied. Line heterosis refers to average heterosis in crosses involving that line. Direct heterosis ( hij ) for each trait differed considerably among crosses. The three crosses showing the highest hij for litter size at birth, W+ X L-W+ (1.78), L+ X W+ (1.28) and L-W+ X L+W- (1.22), possibly had loci contributing directional dominance to litter size with frequencies of parental lines deviating in opposite directions relative to mean gene frequency. The correlation between absolute difference in parental line means and hij for litter size was not significant, suggesting that the magnitudes of absolute differences in parental means were not reliable predictors of divergence in gene frequency. Crossbred performance increased linearly with midparent values for litter size at birth (b = .88 +/- .09, R2 = .92) and dam parturition body weight (b = 1.13 +/- .04, R2 = .99), the latter trait showing an increase (P less than .01) in heterosis as midparent values increased.     

Direct Detection of C4H2 Photochemical Products: Possible Routes to Complex Hydrocarbons in Planetary Atmospheres

The photochemistry of diacetylene (C4H2), the largest hydrocarbon to be unambiguously identified in planetary atmospheres, is of considerable importance to understanding the mechanisms by which complex molecules are formed in the solar system. In this work, the primary products of C4H2's ultraviolet photochemistry were determined in a two-laser pump-probe scheme in which the products of C4H2 photoexcitation are detected by vacuum ultraviolet photoionization in a time-of-flight mass spectrometer. Three larger hydrocarbon primary products were observed with good yield in the C4H2* + C4H2 reaction: C6H2, C812, and C8H3. Neither C6H2 nor C8H3 is anticipated by current photochemical models of the atmospheres of Titan, Uranus, Neptune, Pluto, and Triton. The free hydrogen atoms that are released during the formation of the C8H3 and C8H2 products also may partially offset the role of C4H2 in catalysing the recombination of free hydrogen atoms in the planetary atmospheres.     

Direct and maternal genetic differences between lines of mice selected for body weight and litter size: traits of dams

Genetic differences in performance of dams were estimated by linear contrasts using means of two selected lines of mice and reciprocal F1's, F2's and backcrosses. The lines were selected for increased 6-wk body weight (W) or increased litter size (L). Genetic differences estimated were direct average (gD), direct heterosis (hD), maternal average (gM), progeny average (gP), and progeny heterosis (hP). For dam weight and feed consumption from 12 to 21 d postpartum (pp), gD was the largest genetic difference and favored line W. For litter size, litter weight at birth, litter efficiency (litter weight gain/dam feed consumption) from birth to 12 d pp and within litter mortality from 1 to 21 d pp, gD favored L and, except for hD in litter efficiency, was the most important genetic difference for these traits. Direct heterosis was the only significant difference for litter weight at 21 d pp, litter efficiency from 12 to 21 d pp and within litter mortality at parturition. The gM were larger in W than in L for dam weight and feed consumption, and for litter size and weight at birth, but they were usually of smaller magnitude than gD. The gP were significant only in litter traits measured before 12 d pp and favored W. For no trait measured was hP of consequence. Line differences in dam and litter weight accounted for genetic differences in dam feed consumption. Genetic differences in litter size at birth were not due to line differences in dam weight. The lower mortality within litters nursed by crossbred dams was responsible for hD on litter weight and litter efficiency. Within but not among lines, higher mortality rates were associated with larger litters.     

The effect of cattle grazing on soil physical and chemical properties in a silvopastoral system in the Peruvian Amazon

In a six-year-old peach palm (Bactris gasipaes) plantation, centrosema (Centrosema macrocarpum), a leguminous forage plant, was established as a cover crop which was eventually grazed. This experiment was designed to monitor probable changes in soil physical and chemical properties and measure peach palm fruit production and live-weight gain of cattle grazing this silvopastoral system. The experiment was installed on land that was previously cleared by a D7 bulldozer having a straight blade that mixed the thin layer of topsoil with the acid subsoil (20–40 cm) and severely compacted the soil. The results demonstrated that the centrosema cover crop reduced soil bulk density, increased water infiltration rates and reduced mechanical resistance. In general soil physical properties were improved with the use of Centrosema as forage and cover crop. Soil acidity and aluminum saturation decreased considerably, while potassium concentrations increased. Calcium and magnesium concentrations decreased over time as these minerals were stored in the pasture biomass, translocated to fresh peach palm fruits and/or exported to animals. A strong competition for nutrients was observed between the peach palm plants and Centrosema. The low production of peach palm fruits was in response to mechanized land clearing during initial establishment of the plantation, and also probably due to deficits of N, P, K, Ca and Mg in the soil instead of being a consequence to the presence of cattle. The average increase in live-weight gains of the cattle was at a rate of 445 g/animal/day during the four-years of the study. Such an increase is substantially greater than those registered in the area under traditional grazing systems used in the region. This revised version was published online in August 2006 with corrections to the Cover Date.     

Amazon basin soils: management for continuous crop production

Technology has been developed which permits continuous production of annual crops in some of the acid, infertile soils of the Amazon Basin. Studies in Yurimaguas, Peru, show that three grain crops can be produced annually with appropriate fertilizer inputs. Twenty-one crops have been harvested during the past 8(1/2) years in the same field, with an average annual production of 7.8 tons of grain per hectare. Soil properties are improving with continuous cultivation. The technology has been validated by local farmers, who normally practice shifting cultivation. Economic interpretations indicate large increases in annual family farm income and a high return on the investment of chemical inputs. Other promising land use alternatives include low-input crop production systems, paddy rice production in fertile alluvial soils, and pastures or agroforestry in rolling areas. Stable, continuous food crop production is an attractive alternative to shifting cultivation in humid tropical regions experiencing severe demographic pressures. For each hectare of land managed in a highly productive manner, there may be less need for clearing additional tropical forests to meet food demands.     

Second-order selection for evolvability in a large Escherichia coli population

In theory, competition between asexual lineages can lead to second-order selection for greater evolutionary potential. To test this hypothesis, we revived a frozen population of Escherichia coli from a long-term evolution experiment and compared the fitness and ultimate fates of four genetically distinct clones. Surprisingly, two clones with beneficial mutations that would eventually take over the population had significantly lower competitive fitness than two clones with mutations that later went extinct. By replaying evolution many times from these clones, we showed that the eventual winners likely prevailed because they had greater potential for further adaptation. Genetic interactions that reduce the benefit of certain regulatory mutations in the eventual losers appear to explain, at least in part, why they were outcompeted.     

Post‐exposure rabies prophylaxis for mass bat exposures: Case series and systematic review

To assess and describe the use of rabies post‐exposure prophylaxis (PEP) related to mass bat exposures (MBEs) in the literature and in the state of Rhode Island. Data on MBE events occurring between 2010 and 2016 from the Rhode Island Department of Health (RIDOH) were reviewed to determine PEP usage. For comparison, a systematic review was also performed by searching PubMed, EMBASE, Cochrane Library and Web of Science using the keywords ‘rabies,’ ‘bat’ and ‘exposure.’ There were three MBE events in Rhode Island between 2010 and 2016 in which groups of individuals were asleep during the potential exposure. As a result, RIDOH provided PEP to those involved in the event. In a systematic review of 12 published reports, a variety of settings were involved in MBEs: camping sites, a domestic flight, a neonatal intensive care unit and dormitories. The number of potential rabies exposures ranged from five to 1,429 people. PEP recommendations for assessed persons ranged from 0% to 100% across events (median 21%). The variation in PEP recommendations following a MBE may be dependent on medical need, past precedent or preference. Federal guidance for MBE is needed to add clarity and to minimize the variability in PEP recommendations for such events in the future.     

Alaskan Upper Miocene Marine Glacial Deposits and the Turborotalia pachyderma Datum Plane

ln southeastern Alaska the first marine evidence of widespread glaciation occurs in Miocene sections near the base of the Yakataga Formation. An associated temperature decrease of about 10 degrees C is indicated by the influx of an upper Miocene cold-water planktontic foraminifer, Turborotalia pachyderma, an event that occurred about 13 million years ago.     

Miocene-Pliocene Boundary in the Philippines as Related to Late Tertiary Stratigraphy of Deep-Sea Sediments

Planktonic foraminiferal trends across the Miocene-Pliocene boundary in the Philippines suggest that sections of eight deep-sea cores reported to be Pliocene are actually latest Miocene, and that a marked extinction of discoasters in the deep-sea cores is due to an unconformity, separating Miocene and Pleisto-cene sediments, representing a time gap of some 10 million years of Pliocene time.