Mapping of quantitative trait loci for lactation persistency traits in German Holstein dairy cattle

A whole genome scan to map quantitative trait loci (QTL) for persistency of milk yield (PMY), persistency of fat yield (PFY), persistency of protein yield (PPY) and persistency of milk energy yield (PEY) was performed in a granddaughter design in the German Holstein dairy cattle population. The analysis included 16 paternal half‐sib families with a total of 872 bulls. The analysis was carried out for the first lactation and for the first three lactations combined using univariate weighted multimarker regression. Controlling the false discovery rate across traits and data sets at a level of 0.15 and treating the four persistency traits as different traits revealed 27 significant QTL. A total of 12 chromosomes showed significant QTL effects on a chromosomewise basis. The DGAT1 effect was highly significant for PPY and protein yield. A haplotype analysis using results of previous studies of the same design revealed a co‐segregation of various persistency QTL and QTL affecting health traits like dystocia and stillbirth and functional traits like non‐return rate 90 and somatic cell score.     

大型灯泡贯流式泵站振动研究概述

机组和泵房的振动是泵站运行中最为常见的问题，直接影响泵站的安全可靠运行。对大型灯泡贯流式泵站振动研究现状和抗振安全评价计算分析方法作了详细的介绍，对研究机组振动诱发泵房振动的共振校核法、拟静力法、谐响应求解法和瞬态动力时程法等进行了阐述，并对这几种方法进行了分析比较，就其存在的问题作了总结概述。     

几种甘蓝型油菜幼苗抗旱性特征的比较研究

以EV1、EV2、EV3、EV4、EV5、EV66个抗旱性不同的油菜品种为试验材料,通过不同浓度PEG-6000渗透溶液模拟干旱胁迫处理,研究油菜幼苗的抗旱指数、根长、苗高和根冠比等性状变化,探索在大田环境条件下的苗期生长特征。结果表明,水分胁迫下EV2的抗旱指数最大,EV6的抗旱指数最小;不同浓度PEG胁迫下,各品种差异显著, EV1、EV2、和EV3的根长、苗高和根冠比较大,而EV5和EV6的表现较差。在大田油菜五叶期时,EV2的冠层覆盖面积最大,与其它品种之间存在极显著性差异;总生物量以EV2、EV3和EV1较大,根系干重以EV2较大,而且三者之间存在显著相关性;与抗旱指数进行通径分析,冠层覆盖面积对抗旱性的直接通径系数最大,达到0.5975。冠层覆盖面积的大小反映了早期活力的强弱,早期活力强,相应的品种抗旱指数高,抗旱性强。     

Agglomeration and Competition among Chinese Cities: An Investigation of Taiwanese High‐Tech Foreign Direct Investment

The purpose of this study was to investigate how Taiwanese high‐tech firms' foreign direct investment (FDI) leads to agglomeration and competition among cities in China. By using social network analysis to analyze 1,111 subsidiaries affiliated with 270 multinational corporations (MNCs) during the years between 1988 and 2006 in 30 major Chinese cities, this study finds that: 1) agglomeration in Chinese cities falls into three primary categories: the hub cluster, the communication‐extended cluster, and the computer‐extended cluster. Among the aforementioned clusters, the hub cluster is the most critical in terms of firm location, and therefore is often a high‐priority investment site for Taiwanese high‐tech firms. 2) Chinese cities fall into four primary groups, or “blocks”: the core block, the coast block, the inland block, and the periphery block; these groups were determined by applying a structural equivalence analysis of which members of each type are in competition with one another. By linking the findings of the urban network characteristics to Chinese regional economic policies, this study provides guidance to aid executives in determining the optimal location for future FDI. Implications and suggestions for host country governments in terms of firms' investment strategies are also discussed.     

Generating solitons by phase engineering of a bose-einstein condensate

Quantum phase engineering is demonstrated with two techniques that allow the spatial phase distribution of a Bose-Einstein condensate (BEC) to be written and read out. A quantum state was designed and produced by optically imprinting a phase pattern onto a BEC of sodium atoms, and matter-wave interferometry with spatially resolved imaging was used to analyze the resultant phase distribution. An appropriate phase imprint created solitons, the first experimental realization of this nonlinear phenomenon in a BEC. The subsequent evolution of these excitations was investigated both experimentally and theoretically.     

黄土耕作坡面溅蚀过程中微地形响应特征

[目的]探究60mm/h雨强下不同耕作坡面在溅蚀阶段的微地形变化特征,为黄土坡地水土保持耕作方式布设提供科学依据。[方法]在室内人工模拟降雨试验的基础上,采用激光扫描量测方法分别获取溅蚀前后4种不同耕作方式(人工锄耕、人工掏挖、等高耕作、耙磨整平)黄土坡面微地形相对高程,并对能反映微地形变化的高精度M-ΔDEM进行了分析。[结果]不同耕作措施下坡面微地形均具有较弱的空间变异,其空间分异主要受耕作措施的影响;降雨对微地形具有一定的夷平效应,且不同耕作坡面在1~1.3m尺度范围内均表现出侵蚀平衡现象;不同耕作微地形对溅蚀均具有阻滞作用,其中人工掏挖和等高耕作具有较强的水土保持作用。[结论]微地形水系分维能较好地反映复杂的微地形特征,而坡面耕作方式是溅蚀过程中微地形时空变异分布状况的控制性因子。     

Effect of fertilizers with the new nitrification inhibitor DMPP (3,4-dimethylpyrazole phosphate) on yield and quality of agricultural and horticultural crops

In 1997-1999, 136 field trials were conducted under various soil-climatic conditions in western and southern Europe in order to assess the effects of N fertilizers with the new nitrification inhibitor (NI) 3,4-dimethylpyrazole phosphate (DMPP) on the yield and quality of various agricultural and horticultural crops. Results show that DMPP may increase the mean crop yield (grain yield, winter wheat +0.25 t ha^-1, wetland rice +0.29 t ha^-1, grain maize +0.24 t ha^-1; tuber yield, potatoes +1.9 t ha^-1; corrected sugar yield, sugar beets +0.24 t ha^-1; biomass, carrots +4.9 t ha^-1, lambs' lettuce +1.9 t ha^-1, onions +0.5 t ha^-1, radish +4.6 t ha^-1, lettuce +1.4 t ha^-1, cauliflower +5.2 t ha^-1, leek +1.7 t ha^-1, celeriac +2.2 t ha^-1) and/or improve crop quality (e.g. reduced NO₃^- concentration in leafy vegetables). In some crops, the same yield level as obtained with the control (fertilizer without DMPP) was achieved with one fewer applications of N, or with a reduced N application rate. The positive effect of DMPP on crop yield was especially pronounced at sites with a high precipitation rate or intensive irrigation, and/or light sandy soil. DMPP had a negative effect on the crude protein concentration of winter wheat and on the biomass yield of spring-grown spinach.     

Persistence of Mycoplasma gallisepticum in chickens after treatment with enrofloxacin without development of resistance

The ability of the avian pathogen Mycoplasma gallisepticum to persist despite fluoroquinolone treatment was investigated in chickens. Groups of specific pathogen free chickens were experimentally infected with M. gallisepticum and treated with enrofloxacin at increasing concentrations up to the therapeutic dose. When M. gallisepticum could no longer be re-isolated from chickens, birds were stressed by inoculation of infectious bronchitis virus or avian pneumovirus. Although M. gallisepticum could not be cultured from tracheal swabs collected on several consecutive sampling days after the end of the enrofloxacin treatments, the infection was not eradicated. Viral infections reactivated the mycoplasma infection. Mycoplasmas were isolated from tracheal rings cultured for several days, suggesting that M. gallisepticum persisted in the trachea despite the enrofloxacin treatment. The minimal inhibitory concentration (MIC) of enrofloxacin for most of the re-isolated mycoplasmas was the same as that of the strain with which the birds were inoculated. Furthermore, no mutation could be detected in the fluoroquinolone target genes. These results suggest that M. gallisepticum can persist in chickens without development of resistance despite several treatments with enrofloxacin.     

Proteomic survey of bovine neutrophils

Mastitis is a major economic concern for the dairy industry. Conditions such as parturition cause a transient immunosuppression that leads to increased incidence of mastitis. One facet of periparturient immunosuppression is a functional impairment of the blood and milk neutrophils in dairy cows. To better understand the biology of the bovine neutrophil we report the first proteomic analysis of the bovine neutrophil. We have identified over 250 proteins using one-dimensional electrophoresis followed by reverse-phase chromatography in line with electrospray tandem mass spectrometry. A large number of metabolic proteins were identified, including most of the enzymes required for generation of NADPH and ATP. In addition, many proteins were identified that participate in cell mobility and phagocytosis. All the bovine members of the cathelicidin family were identified, as well as other proteins with immunological functions. Proteins important for cell signaling, vesicular transport, control of apoptosis and other functions were identified giving an overview of the bovine neutrophil proteome.