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Doru Morar Gheorghe Dărăbuş Mirela Imre Marius Stelian Ilie Kálmán Imre 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(2):200-204
This case study describes the first genetically confirmed and clinically manifested autochthonous Ehrlichia canis infection in a 9‐year‐old female mixed‐breed dog from Romania. Health screening of the dog included clinical examination, evaluation of stained peripheral blood smear and hematologic variables, as well as serologic testing and molecular analysis. Clinical signs included fever, apathy, dehydration, pale mucous membranes, and weakness. The microscopic examination of the blood smear and immunologic assays for Borrelia burgdorferi, Anaplasma phagocytophilum, and E canis antibodies, and for Dirofilaria immitis antigen yielded negative results. Hematologic abnormalities included moderate nonregenerative anemia, leucopenia with neutropenia, and moderate thrombocytopenia. The biochemical abnormalities identified were hypoalbuminemia, and mildly increased serum enzyme activities of AST and ALT. In addition, increased urea and creatinine levels associated with low urine specific gravity and proteinuria were also present. Nested PCR amplification of the partial E canis 16S rRNA gene demonstrated the presence of this rickettsial pathogen in the dog's blood, which subsequently was confirmed through sequencing based on the 100% homology with GenBank deposited E canis isolates. After specific treatment with doxycycline (10 mg/kg, orally, SID) for one month, the proteinuria, and hematologic and serum biochemical abnormalities with the exception of mild azotemia resolved. This report supports the geographical expansion of canine ehrlichiosis caused by E canis in nonendemic regions of Europe. 相似文献
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Clinical signs, humoral and cellular immune responses, and microscopic and gross tissue alterations resulting from acute experimental Ehrlichia canis infection in dogs were studied. Four dogs were inoculated with E. canis and four were used as uninfected controls. After a 10-14-day incubation period, infected dogs developed pyrexia up to 41 degrees C for 6-8 days. Antibody titers to E. canis antigen were demonstrable in all inoculated dogs at 30 days post-infection. Necropsy of infected animals revealed pale mucous membranes, generalized lymphadenopathy, splenomegaly, edema and ascites. Microcopically, the main lesions were: lymphoreticular hyperplasia in cortical areas of lymph nodes and spleenic white pulp, periportal accumulation of mononuclear cells and centrolobular fatty degeneration of the liver. Kidneys presented with glomerulonephritis characterized by interstitial mononuclear infiltration. Immunophenotyping of lymphocytes from lymph nodes and spleen sections displayed alterations in IgG, IgM, CD3+ and CD8+ cells population in infected dogs. 相似文献
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Cekani M Papa A Kota M Velo E Berxholi K 《Veterinary journal (London, England : 1997)》2008,175(2):276-278
The prevalence of Coxiella burnetii antibodies in domestic ruminants in Albania has been investigated. A total of 1656 serum samples taken from sheep, goats, and cattle housed on farms located in 20 different districts were tested by ELISA for the presence of specific antibodies to C. burnetii phase I and II antigens. Specific IgG antibodies were detected in 9.1% of the animals from both lowland and mountainous areas. In total, a slightly higher percentage of antibodies was detected in sheep and goats (9.8%) than in cattle (7.9%). 相似文献
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Context
Despite calls for landscape connectivity research to account for spatiotemporal dynamics, studies have overwhelmingly evaluated the importance of habitats for connectivity at single or limited moments in time. Remote sensing time series represent a promising resource for studying connectivity within dynamic ecosystems. However, there is a critical need to assess how static and dynamic landscape connectivity modelling approaches compare for prioritising habitats for conservation within dynamic environments.Objectives
To assess whether static landscape connectivity analyses can identify similar important areas for connectivity as analyses based on dynamic remotely sensed time series data.Methods
We compared degree and betweenness centrality graph theory metric distributions from four static scenarios against equivalent results from a dynamic 25-year remotely sensed surface-water time series. Metrics were compared at multiple spatial aggregation scales across south-eastern Australia’s 1 million km2 semi-arid Murray–Darling Basin and three sub-regions with varying levels of hydroclimatic variability and development.Results
We revealed large differences between static and dynamic connectivity metric distributions that varied by static scenario, region, spatial scale and hydroclimatic conditions. Static and dynamic metrics showed particularly low overlap within unregulated and spatiotemporally variable regions, although similarities increased at coarse aggregation scales.Conclusions
In regions that exhibit high spatiotemporal variability, static connectivity modelling approaches are unlikely to serve as effective surrogates for more data intensive approaches based on dynamic, remotely sensed data. Although this limitation may be moderated by spatially aggregating static connectivity outputs, our results highlight the value of remotely sensed time series for assessing connectivity in dynamic landscapes.7.
Gabriela Noronha de Toledo Pamela Rodrigues Reina Moreira Daniele Santos Rolemberg Andréia Coutinho Facin Julio Edward Hough Monteiro Mareliza Possa de Menezes Maria Eduarda Bastos Andrade Moutinho da Conceição Felipe Augusto Ruiz Sueiro Rosemeri de Oliveira Vasconcelos Paola de Castro Moraes Mirela Tinucci Costa Andrigo Barboza de Nardi 《Topics in companion animal medicine》2018,33(2):35-39
Osteosarcoma is the most commonly reported bone tumor in dogs, typically affecting the axial and appendicular skeleton. Involvement of the paranasal sinuses and the nasal cavity is extremely rare. This report describes a 6-year-old, female, cross-breed dog presented with signs of 90 days duration and diagnosed with a mass located in the right nasal cavity. Radiography, rhinoscopy, and computed tomography findings suggested the presence of an intranasal tumor. Osteosarcoma in the nasal cavity was diagnosed based on histopathological examination and clinical and imaging findings. Survival time was 12 months from diagnosis. 相似文献
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Mirela Colleoni‐Sirghie Jean‐Luc Jannink Igor V. Kovalenko Jenni L. Briggs Pamela J. White 《Cereal Chemistry》2004,81(4):434-443
Rheological properties of raw oat flour slurries were determined in experimental high β‐glucan (≤7.8%) and traditional oat lines (4–5% β‐glucan) grown in two consecutive years. Three different media were used to disperse oat flours: deionized water, silver nitrate solution (to inactivate endogenous enzymes), and alkali solution (to solubilize both water‐soluble and water‐insoluble β‐glucans). Significant correlations (P < 0.05) between viscosity of slurries and β‐glucan concentration obtained in either deionized water (r = 0.833), silver nitrate (r = 0.940), or alkali (r = 0.896) solutions showed that β‐glucans were the main contributor to oat extract viscosity. The highest correlation was obtained in silver nitrate solution, suggesting that inactivating endogenous enzymes is important to obtain high correlations. Predictive models of oat β‐glucan concentration based on the viscosity profile were developed using partial least squares (PLS) regression. Prediction of β‐glucan concentration based on viscosity was most effective in the silver nitrate solution (r = 0.949, correlation coefficient of predicted vs. analyzed β‐glucans) and least effective in the alkali solution (r = 0.870). These findings demonstrate that the β‐glucan in oat could be predicted by measuring the viscosity of raw flours in silver nitrate solution, and this method could be used as a screening tool for selective breeding. 相似文献
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Experimental oat lines high in β‐glucan (6–7.8%) and traditional lines (3.9–5.7% β‐glucan) were used to evaluate the effect of β‐glucan on pasting (by rapid viscoanalysis) and thermal properties (by differential scanning calorimetry) of oat flours. Significant correlations established between β‐glucan concentration and the pasting parameters after amylolysis demonstrated the role of β‐glucans in oat pasting. The relative decrease of peak viscosity (PV) observed after enzymatic removal of β‐glucans was correlated with β‐glucan concentration (r = 0.880, P < 0.010) and reconfirmed their contribution to pasting. A significant increase of PV with β‐glucan concentration obtained under conditions of either autolysis (deionized water used for dispersion) (r = 0.89, P < 0.010) or inhibition (silver nitrate solution used for dispersion) (r = 0.91, P < 0.001) might be explained by an increase in water retention capacity caused by the β‐glucans. Predictive models of β‐glucan concentration based on the whole pasting profile or selected profile regions were developed using partial least squares (PLS) regression.Prediction of β‐glucan based on the whole profile obtained in the silver nitrate solution was the most effective (r = 0.93, correlation coefficient of predicted vs. analyzed β‐glucans, P < 0.050). No correlations were observed between the thermal properties of oat flours and the β‐glucan concentration. 相似文献
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